Method for preparing succinic acid by coupling fermentation and film separation unit

A succinic acid and membrane separation technology, applied in the field of bioengineering, can solve the problems of substrate and other nutrient element loss, difficult separation of product and substrate, consumption of large acid-base regeneration resin, etc., to achieve easy automatic control and industrial The effects of modernization, significant social and economic benefits, and increased production intensity

Inactive Publication Date: 2008-07-09
NANJING UNIV OF TECH
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Problems solved by technology

[0007] However, in the current research on the preparation of organic acids by reaction-separation coupled fermentation based on membrane separation technology, microfiltration and ultrafiltration membranes based on sieving principles are mostly used as a single separation component. This type of design can only achieve the separation of cells and fermentation broth. , it is difficult to achieve the separation of products and substrates, which inevitably results in the loss of unconsumed substrates and other nutrients in the fermentation broth. In order to solve this problem, predecessors combined ion Exchange, chemical extraction, electrodialysis and other means recover unconsumed substrates, but these means may consume a large amount of acid-base...

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  • Method for preparing succinic acid by coupling fermentation and film separation unit

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Embodiment 1

[0034] (1) Seed medium: glucose 10g / L, yeast extract 5g / L, peptone 5g / L, NaCl 1g / L, NaHCO 3 10g / L, Na 2 HPO 4 9~15g / L,KH 2 PO 4 4~12g / L, (add agar 20g / L when cultivating on a slant), pass through N 2 :CO 2 = 4:1 mixed gas for 2 minutes, sterilized at 121°C for 20 minutes, and cooled;

[0035] (2) Fermentation medium: glucose 40g / L, yeast extract 10-50g / L, peptone 10-50g / L, MnCl 2 0.05g / L, FeCl 2 0.05g / L, CaCl 2 0.05g / L, K 2 HPO 4 2.0g / L, vitamins added folic acid 5mg / L, biotin 5mg / L, vitamin B 12 5mg / L, lipoic acid 5mg / L, niacin 5mg / L, pantothenic acid 5mg / L, thiamine 5mg / L, vitamin B 6 5mg / L, pH 7.0;

[0036] (3) After the Actinobacillus succinogenes strain whose preservation number is CGMCC No.1716 is streaked on the seed slant medium, it is cultivated in an anaerobic incubator, and the ratio of mixed gas in the control incubator is: N 2 :CO 2 :H 2 =8: 1: 1, the bacterial strain after cultivating 36h is connected in the 100ml serum bottle that contains...

Embodiment 2

[0040] (1) Seed culture medium and fermentation medium are all the same as in Example 1.

[0041] (2) Prepare fermented seeds from the Actinobacillus succinogenes strain with the preservation number CGMCC No.1716, and the method is the same as in Example 1. When using a 5L fermenter for cultivation, the liquid volume of the medium is 3L, the inoculum size is 4% (v / v), the stirring speed is at 300rpm, 37°C, the pH is adjusted to 6.8 with 280g / L sodium carbonate, and the ventilation rate of carbon dioxide in the fermenter It is 1.0L / min. After 11 hours of fermentation, the production intensity of succinic acid has reached 2.2g / L h. At the end of the logarithmic growth phase of bacterial growth, the total concentration of succinic acid, formic acid and acetic acid accumulated in the system has reached 29g / L. At this time, the fermentation The glucose concentration in the liquid is 11g / L, and at this time, it is ready to be coupled with an ultrafiltration membrane for semi-contin...

Embodiment 3

[0046] The preservation number is the bacterial strain of CGMCC No.1716 adopting the same method as Example 1 to prepare fermenter seeds. When cultivating in a 5L fermenter, the liquid content of the medium is 3L, and the concentration of glucose in the fermentation medium is 25g / L. With embodiment 1, adopt 10N sodium hydroxide to adjust pH 6.80, pass into CO 2 , the ventilation rate is 0.75L / min, the anaerobic environment of the fermentation system is maintained, the inoculum size is 5% (v / v), the rotation speed is 250r / min, and after culturing at 37°C for 10.5 hours, the production intensity of succinic acid has reached 1.7 g / L·h, the total concentration of succinic acid, formic acid, and acetic acid accumulated in the system has reached 23g / L, and the concentration of glucose is only 2g / L. It is ready to be coupled with a microfiltration membrane for continuous fermentation.

[0047] Adopt the tubular ceramic microfiltration membrane of 0.14 μm through 4% sodium hydroxide d...

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Abstract

The invention discloses a process for preparing succinic acid through coupling fermentation and membrane separation units, the process for preparation comprises following steps: firstly, inoculating fermentation seeds of bacteria strains which produce the succinic acid into a fermentation tank to culture, then, coupling microfiltration or ultrafiltration membrane separation units with the fermentation tank to realize semi-continuously or continuously preparing the succinic acid with fermentation, separating out part of fermentation liquor, releasing from the control of metabolites, returning somatic cells and components which are cut off by the membrane separation units back into the fermentation tank and continuingly fermenting, continuingly using substrate which is not consumed in the fermentation liquor which is nanofiltrated, reclaimed, and separated to ferment, and simultaneously adding the fermentation culture medium to keep the volume of the fermentation liquor in the fermentation tank constant. The invention not only can release from the control of the metabolites and increase the production strength of the succinic acid, but also uses reducing sugar which is not consumed in the liquor which is nanofiltrated, reclaimed, and separated, simultaneously concentration is available, the technical condition is moderate, environmental pollution is little, which is easy to control automatically and has wide social and economic benefits.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, in particular to a method for preparing succinic acid by coupling fermentation with a membrane separation unit. Background technique [0002] Succinic acid (Succinic Acid, Butanedioic Acid), also known as succinic acid, is an important dibasic organic carboxylic acid, which can be used as an intermediate in the synthesis of complex organic compounds, and is widely used in pharmaceuticals, food processing, synthetic materials, surfactants , protective coatings, dyes and other industries. However, the traditional chemical synthesis of succinic acid has limited the wide application of succinic acid as a basic chemical raw material due to reasons such as cost and environmental pollution. The production of succinic acid by fermentation method uses low-cost renewable biomass and greenhouse gas carbon dioxide as raw materials, with less pollution and low energy consumption. Therefore, the resear...

Claims

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Application Information

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IPC IPC(8): C12P7/46C12R1/01
Inventor 姜岷吴昊韦萍左鹏雷丹陈可泉姚忠
Owner NANJING UNIV OF TECH
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