Yarrowia lipolytica strain and method thereof for synthesizing erythritol

A technology of Yarrowia lipolytica and yeast strains, which is applied in the biological field, can solve the problems of increasing erythritol purification cost, erythritol energy consumption, and deepening the color of fermentation medium, and achieve high synthesis of erythritol capacity, energy saving, and improvement in conversion rate
CN103374534AActive Publication Date: 2013-10-30SHANGHAI JIAO TONG UNIV +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANGHAI JIAO TONG UNIV
Publication Date
2013-10-30

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Abstract

The invention discloses a yarrowia lipolytica strain and a method thereof for synthesizing erythritol. The yarrowia lipolytica strain is yarrowia lipolytica BLC13 CGMCC NO. 7326. The method for synthesizing the erythritol by the yarrowia lipolytica strain comprises the following steps of: taking a carbon source with an initial fermentation concentration of 100-400g / L, a nitrogen source with an initial fermentation concentration of 2-35g / L and inorganic salt as raw materials, treating for 30 minutes at 80-90 DEG C, cooling down and subsequently inoculating the yarrowia lipolytica strain, carrying out continuous fermentation or in-batch fed-batch fermentation under an aerobic condition, and after the fermentation is accomplished, purifying the erythritol from a fermentation liquid. The yarrowia lipolytica strain disclosed by the invention is used for synthesizing the erythritol from glucose, the conversion rate is high, and the Chinese erythritol standard is met.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a Yarrowia lipolytica strain and a method for synthesizing erythritol. Background technique

[0002] Erythritol (also known as butylene glycol) is a tetravalent polyol with a molecular weight of 122.1 Daltons. It exists in many fruits, vegetables and brewed foods (such as pears, glucose and other fruits and wines), but the content rare. Because erythritol has the advantages of zero calories, no dose limit, and pure biological fermentation preparation, erythritol is more widely used in food, and has been widely used in candies, baked goods, sugar-free drinks and other foods.

[0003] For example: the Chinese invention patent whose application number is CN200710014055.8 discloses a method for making sandwich chocolate containing erythritol; the Chinese invention patent whose application number is CN201110420379. Sugar drink and its preparation method; the Chinese inventio...

Claims

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