Bacterial strain for degrading polycyclic aromatic hydrocarbon and application of bacterial strain cooperated with surface active agent in soil remediation

A technology of surfactants and polycyclic aromatic hydrocarbons, which is applied in the fields of environmental engineering and microbial engineering, can solve the problems of low bioavailability of PAHs and difficult microbial utilization, and achieve a wide range of degradation substrates, broad application potential, and high degradation rate. Effect

Inactive Publication Date: 2013-03-27
杭州韦尔茂通环境技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, the bioavailability of PAHs in soil is generally low. It is generally believed that PAHs or other substrates dissolved in the liquid phase can be utilized by microorganisms. On the contrary, if they are adsorbed on the soil, they are difficult to be utilized by microorganisms.

Method used

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  • Bacterial strain for degrading polycyclic aromatic hydrocarbon and application of bacterial strain cooperated with surface active agent in soil remediation
  • Bacterial strain for degrading polycyclic aromatic hydrocarbon and application of bacterial strain cooperated with surface active agent in soil remediation
  • Bacterial strain for degrading polycyclic aromatic hydrocarbon and application of bacterial strain cooperated with surface active agent in soil remediation

Examples

Experimental program
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Effect test

Embodiment 1

[0049] The separation and screening method of tzyx3 provided by the invention.

[0050]Collect surface soil samples at a distance of 5-20 cm from the solid waste dismantling site, and use them as bacteria sources for natural enrichment culture. Add 10 g of glucose, 10 g of tryptone, and 5 g of yeast powder to a certain amount of distilled water to prepare a culture The solution was sterilized at 121°C for 20 minutes, cultured on this medium for three days, and then prepared an inorganic salt base medium, adding naphthalene, phenanthrene, fluoranthene, pyrene, benzanthracene, and benzopyrene. Polycyclic aromatic hydrocarbon compounds, the above-mentioned addition of naphthalene, phenanthrene, fluoranthene, pyrene, benzanthracene, and benzopyrene is 150mg / mL, cultured on this medium for three days, and a strain of bacteria was isolated by dilution coating and named as tzyx3; the bacterium was preserved in the "China Center for Type Culture Collection" on June 24, 2012, with the ...

Embodiment 2

[0053] Tzyx3 synergistically degrades polycyclic aromatic hydrocarbons in polluted soil with humic acid, a surfactant.

[0054] Select 100g of PAH-contaminated soil, mix tzyx3 with the soil at an amount of 2.0% (mass percentage), use humic acid as the surfactant, add 0.1% of the dry weight of the polluted soil, add a small amount of water to keep the soil moisture content At about 15%, put it into a constant temperature shaker, control the culture temperature at a constant temperature of 25°C, shake and cultivate and repair for 15 days, and then the composite polluted soil can be repaired and the repaired soil can be obtained.

[0055] figure 1 - Image 6 The kinetic degradation curves of PAHs naphthalene, phenanthrene, fluoranthene, pyrene, benzanthracene, and benzopyrene over time in different treatment soils were respectively shown. The four different treatments were control polluted soil sample (CK), no addition of humic acid, no inoculation; inoculation of PAHs degrading...

Embodiment 3

[0058] Different from Example 2, the surfactant of this scheme is rhamnolipid, and data monitoring shows that after 15 days, the removal rate of rhamnolipid+YA treated soil polycyclic aromatic hydrocarbons is:

[0059] Naphthalene 94.2%, phenanthrene 92.1%, fluoranthene 71.6%, pyrene 83.8%, benzanthracene 83.6%, benzopyrene 73.8%.

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Abstract

The invention belongs to the technical fields of environmental engineering and microbial engineering, and in particular relates to Yarrowia lipolytica tzyx3 for degrading polycyclic aromatic hydrocarbon and application of the Yarrowia lipolytica tzyx3 cooperated with a surface active agent in soil remediation. The preservation number of the Yarrowia lipolytica tzyx3 in China center for type culture collection is CCTCC NO: M 2012241, and the Yarrowia lipolytica tzyx3 is capable of degrading the polycyclic aromatic hydrocarbon. The Yarrowia lipolytica tzyx3 has synergistic effect with the surface active agent and can be used for remediation of soil polluted by the polycyclic aromatic hydrocarbon.

Description

technical field [0001] The invention belongs to the technical fields of environmental engineering and microbial engineering. In particular, a strain of Yarrowia lipolytica ( Yarrowia lipolytica ) tzyx3 and its synergy with surfactants in soil remediation. Background technique [0002] Polycyclic aromatic hydrocarbons (PAHs) are a class of persistent organic pollutants (POPs). PAHs pollution sources mainly come from waste machine oil, lubricating oil of electrical products, and hydrocarbon-containing substances such as wood, organic polymer compounds, paper, etc. from the dismantling of waste metal through incomplete combustion or thermal decomposition in a reducing atmosphere. And generated. Because PAHs and their derivatives have carcinogenicity, mutagenicity and teratogenicity, as well as acute toxicity and sublethal effects, they have attracted great attention. [0003] The remediation of polycyclic aromatic hydrocarbons (PAHs) contaminated soil is a research hotspot ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16B09C1/10C12R1/645
Inventor 张昕欣于红艳奚立民
Owner 杭州韦尔茂通环境技术有限公司
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