Process for producing erythritol by utilizing Yarrowia lipolytica strains

A technology for erythritol and yeast strains is applied in the field of fermentation engineering, which can solve the problems of long stirring and blasting time, low production efficiency, easy occurrence of bacterial contamination, etc., so as to shorten the fermentation time, improve the production efficiency, and satisfy the product quality. Effect

Inactive Publication Date: 2012-02-15
FUTASTE PHARM CO LTD
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Problems solved by technology

[0005] The object of the present invention is to provide a kind of technology that utilizes Yarrowia lipolytica strain to produce erythritol, to solve the prior art stirring and blowing time is long, power consumption is high, prone to infection, low production efficiency, high cost

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Embodiment Construction

[0059]The R&D center of our company screened and obtained Yarrowia lipolytica in soil or honeycomb, and obtained erythritol-producing strains after artificial propagation, which were used to produce erythritol. The Yarrowia lipolytica The process steps of cultivating yeast strains and producing erythritol using the strains are: strain preparation → primary and secondary seed cultivation → fermentation to obtain fermented liquid → ceramic membrane filtration → nanofiltration → evaporation and concentration → activated carbon decolorization → ionization Exchange→vacuum crystallization→assisted crystal→centrifugation→drying→sieving→packaging. The mother liquor obtained by centrifugation can reach the standard of the ceramic membrane filtrate after being purified by nanofiltration, so it can be mixed into the ceramic membrane filtrate to enter the next process, which greatly improves the product yield. Its specific production process steps are as follows:

[0060] 1. Preparation ...

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Abstract

The invention relates to a process for producing erythritol by utilizing Yarrowia lipolytica strains, belonging to the technical field of fermentation engineering. In the process, artificial breeding and reproduction is carried out on Yarrowia lipolytica screed in nature so as to produce erythritol, wherein the steps of strain culture of Yarrowia lipolytica and production of erythritol with the strains comprise: strain preparation, primary and secondary seed culture, fermentation to obtain fermentation broth, ceramic membrane filtration, nanofiltration, evaporation and concentration, activated carbon decolorization, ion exchange, vacuum crystallization, auxiliary crystallization, centrifugation, drying, screening and package. In the invention, a mother liquid obtained by centrifugation reaches the standard of ceramic membrane filtrate after being subjected to nano filtration and purification, thus the ceramic membrane filtrate can be mixed and enters into the next process, thereby greatly improving the yield of the product; and the purity of the finished product is more than 99.5%, and the moisture of the finished product is about 0.16%. The process has the advantages of low production cost, short fermentation period, high production efficiency and the like.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, and in particular relates to an erythritol-producing bacterium and a process for producing erythritol using the strain. Background technique [0002] With the increasing number of obese patients around the world, people's demand for non-caloric sweeteners is increasing. As a zero-calorie, pure natural healthy sweetener, erythritol has gradually become popular in Europe and the United States where there are many obese patients. [0003] At present, the enterprises that produce erythritol in my country are mainly two companies, China Food Gorui and Baolingbao. They both adopt Candida lipolytica for fermentation and production, and the strain comes from China Food Fermentation Industry Research Institute. According to the patent CN1932002 (application number: 200510102929.6) of the China Food and Fermentation Industry Research Institute, the fermentation time of this strain is abou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/02C12R1/645
Inventor 邱学良孙鲁杜瑞锋黄伟红李毅姜希生
Owner FUTASTE PHARM CO LTD
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