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Near-infrared second window emission down-conversion nanometer fluorescence probe and synthesis method thereof

A nano-fluorescence probe and near-infrared technology, which is applied in the direction of luminescent materials, fluorescence/phosphorescence, chemical instruments and methods, etc., can solve the problems of the difference in emission efficiency of rare earth ions, the difference between absorption cross section and energy level matching, and improve the sensitivity As well as the effect of accuracy and broad application prospects

Inactive Publication Date: 2018-03-23
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the difference between the absorption cross section and energy level matching of the rare earth ions doped by the down conversion material, the emission efficiency of different rare earth ions is very different.

Method used

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  • Near-infrared second window emission down-conversion nanometer fluorescence probe and synthesis method thereof
  • Near-infrared second window emission down-conversion nanometer fluorescence probe and synthesis method thereof
  • Near-infrared second window emission down-conversion nanometer fluorescence probe and synthesis method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0033] NaGdF 4 @NaYbF 4 :Ho(15%)@NaYF 4 :Yb(10%)@NaNdF 4 : Preparation of Yb(10%) down-conversion nanocrystals with 1155 nm emission in the second near-infrared window. Specific steps are as follows:

[0034] (1) Preparation of the shell precursor. Preparation of Gd-OA (0.1 M) precursor: Take a 100 mL three-necked round-bottomed flask as a reaction vessel, add 2.5 mmol GdCl 3 , 10 mL oleic acid (OA), 15 mL octadecene (ODE). Heating to 140 °C for 1 h under vacuum and magnetic stirring conditions finally yielded a clear and transparent Gd-OA (0.1 M) precursor.

[0035] Preparation of Yb, Ho-OA (0.1 M) precursor: Take a 50 mL three-neck round bottom flask as the reaction vessel, add 2.5 mmol ReCl 3 (Re: 85% Yb, 15% Ho), 10 mL oleic acid (OA), 15 mL octadecene (ODE). Heating to 140 °C under vacuum and magnetic stirring conditions and maintaining for 1 hour, the clear and transparent Gd, Yb, Er-OA (0.1M) precursors were finally obtained.

[0036] Preparation of Y, Yb-OA (...

Embodiment 2

[0049] NaGdF 4 @NaGdF 4 :Yb / Er(18 / 2 %)@NaYF 4 :Yb(10%)@NaNdF 4 : Preparation of Yb(10%) down-converting nanocrystals with 1525 nm emission in the second near-infrared window. Specific steps are as follows:

[0050] (1) Preparation of the shell precursor. Preparation of Gd-OA (0.1 M) precursor: Take a 100 mL three-necked round-bottomed flask as a reaction vessel, add 2.5 mmol GdCl 3 , 10 mL oleic acid (OA), 15 mL octadecene (ODE). Heating to 140 °C for 1 h under vacuum and magnetic stirring conditions finally yielded a clear and transparent Gd-OA (0.1 M) precursor.

[0051] Preparation of Gd, Yb, Er-OA (0.1 M) precursors: Take a 50 mL three-necked round-bottomed flask as a reaction vessel, add 2.5 mmol ReCl 3 (Re: 80% Gd, 18% Yb, 2% Er), 10 mL oleic acid (OA), 15 mL octadecene (ODE). Heating to 140 °C for 1 hour under vacuum and magnetic stirring conditions, finally obtained clear and transparent Gd, Yb, Er-OA (0.1 M) precursors.

[0052] Preparation of Y, Yb-OA (0.1...

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Abstract

The invention belongs to the technical field of biological nano-materials, and concretely relates to a near-infrared second window emission down-conversion nanometer fluorescence probe and a synthesismethod thereof. The fluorescence probe comprises an inert core layer, a down-conversion luminescence center shell layer, an energy transfer shell layer and an energy absorbing shell layer; the inertcore layer is used for adjusting the size of whole nano-crystals; the above down-conversion luminescence center is activated ion-doped rare earth nano-crystals; the energy transfer layer is used for transferring absorbed energy to the luminescence center, and the relaxation time of luminescence ions is extended or shortened by changing the thickness of the energy transfer layer in order to changethe fluorescence lifetime of the nano-crystal fluorescence probe, so the controllability and the adjustability of the fluorescence lifetime are achieved; and the energy absorbing layer is used for absorbing external excitation lights. The probe can be used for simultaneous in-situ detection of multiple signal molecules in deep tissues, further improves the detection sensitivity and the detection accuracy, and has broad application prospects in the fields of protein expression, high-throughput screening of biological samples and multi-channel biological detection.

Description

technical field [0001] The invention belongs to the technical field of nano-biological materials, and in particular relates to a near-infrared second window emission down-conversion nano-fluorescent probe with adjustable lifetime and a synthesis method thereof. Background technique [0002] Nowadays, imaging technology has become a standard test method for medical diagnosis. With the development of science and technology, while medical imaging technology is becoming more and more accurate, it is also necessary to provide more specific biological characteristics in order to help us intuitively understand diseases , as well as the screening of treatment methods, to provide a clearer basis for efficacy evaluation. Fluorescence imaging has been widely used in the fields of life science and biotechnology due to the advantages of real-time, non-invasive, high resolution, and the ability to use wavelengths to achieve multiple detection. In the past studies, researchers mainly stud...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02C09K11/85G01N21/64
Inventor 张凡凡勇
Owner FUDAN UNIV
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