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SERS biological probe and method for making same

A biological probe, silica technology, applied in biological testing, material testing, Raman scattering, etc., can solve the problems of difficult Raman signal molecules, detection sensitivity limitations, etc., to achieve a wide range of universal, good biological phase Capacitance and high detection sensitivity

Inactive Publication Date: 2008-07-09
CAPITAL NORMAL UNIVERSITY
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Problems solved by technology

In the current SERS research, most of the Raman signal molecules are directly combined with the substrate composed of spherical gold and silver nanoparticles to enhance the Raman signal [Science 2002, 297, 1536; Anal.Chem.1999, 71, 4903; Chem .Phys.Lett.1981,82,355.], the shortcoming of this method is: (1) must use gold, silver particle to prepare granular film as the detection substrate of SERS; (2) a Raman signal molecule can only be combined in For detection on one molecule, it is difficult to realize multiple Raman signal molecules combined on one molecule for detection at the same time, so the detection sensitivity is greatly limited

Method used

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  • SERS biological probe and method for making same

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preparation example Construction

[0029] 4) Preparation of SERS bioprobes: SiO coated with gold or silver nanoparticles prepared in step 3) 2 Core-shell particle SiO 2 @nano-Au or SiO 2 The surface of @nano-Ag adsorbs Raman signal molecules, and then forms a nano-silica layer with a controllable thickness to form composite nanoparticles (SiO2) with a "sandwich" structure and Raman signal molecules 2 @nano-Au@SiO 2 or SiO 2 @nano-Ag@SiO 2 ), so that multiple Raman signal molecules can be wrapped in the "sandwich layer" of gold and silver nanoparticles in composite particles; finally, biological probe molecules (DNA probes or antibody molecules) are modified on the surface of composite particles to prepare A SERS bioprobe that can be used for sensitive detection of biomolecules by SERS has been developed.

[0030] Commonly used, silicon dioxide (SiO 2 ) particles can be carried out with reference to literature [J.Colloid Interface Sci.1968,26,62.]; gold and silver nanoparticles of different shapes (spheric...

Embodiment 1

[0033] Example 1. Taking gold nanorods as an example to prepare a new type of SERS immunoprobe:

[0034] 1) Preparation of gold nanorods (Au rod ); reference (J. Colloid Interface Sci. 1968, 26, 62.) Preparation of silica nanoparticles.

[0035] 2) SiO 2 @Au rod Preparation: Disperse 105 mg of the prepared silica particles into 1000 mL of absolute ethanol, add 20 mL of 3-aminopropyl-trimethoxysilane under stirring, and react for 10 min to obtain aminated silica particles. Add 30 mL of 1.8 mol / L PVP solution to the prepared gold nanorod particle solution, centrifuge after 20 min, and add it to the aminated SiO 2 After 1 min, the reaction solution was centrifuged, washed with pure water and ethanol, ultrasonicated, and then centrifuged to remove excess PVP, so that the gold nanorods were immobilized on the silica nanospheres.

[0036] 3) Raman signaling molecules (such as p-mercaptoaniline, 4-ATP) in SiO 2 @Au rod Fixation of particle surface: add 1.5mol / L 4-ATP solution t...

Embodiment 2

[0049] Taking triangular silver nanoparticles as an example to prepare a new type of SERS immunoprobe:

[0050] Except for preparing triangular silver nanoparticles instead of gold nanorod particles with reference to the literature (Adv. Mater. 2005, 17, 412.), the rest of the preparation process is the same as that in Example 1.

[0051] SERS detection of human IgG (h-IgG) using SERS immunoprobes prepared from triangular silver nanoparticles:

[0052] Except that the SERS immunoprobe prepared with triangular silver nanoparticles was used instead of the SERS immunoprobe prepared with gold nanorod particles, the rest of the detection process was the same as that in Example 1. The results showed that the detection limit for human IgG reached 15 pg / g / g mL.

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Abstract

The invention discloses a SERS biological probe and a preparation method thereof. The SERS biological probe provided by the invention comprises a SiO2 core and a metal nanoparticle layer on the surface of the SiO2 core, wherein a plurality of Raman signal molecules connected with the metal nanoparticle layer; a SiO2 layer is also provided outside the metal nanoparticle layer; the Raman signal molecules are positioned between the metal nanoparticle layer and the SiO2 layer; the SiO2 layer surface is modified with biological probe molecules; and the metal nanoparticle layer is Au nanoparticle layer or Ag nanoparticle layer. The SERS biological probe provided by the invention has wide generality, and has wide application prospect in identification and detection of biological molecules (DNA molecules and protein molecules), rapid disease diagnosis, biomedical imaging technology as well as in the fields of serious disease treatment, food hygiene, environmental monitoring, etc.

Description

technical field [0001] The invention relates to a novel surface-enhanced Raman spectroscopy (SERS) biological probe and a preparation method thereof. Background technique [0002] In recent years, laser Raman spectroscopy has become a commonly used spectroscopy method to study the structure of biomolecules, especially in the study of the structure and conformation of proteins in aqueous solutions. Raman technology has the following advantages in the study of biological samples: one is that it can directly obtain the information of groups and chemical bonds at the molecular level and the influence of the microenvironment on the structure of biological samples; the other is that it does not destroy the structure of the sample, Can be used for the study of aqueous systems. Based on these characteristics, it occupies an important position in the study of biological systems. However, the low signal intensity of conventional Raman spectroscopy limits its application in various f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65G01N33/52
Inventor 马占芳
Owner CAPITAL NORMAL UNIVERSITY
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