Method for detecting bacillus-like marker by utilizing small polymer of nanoparticles

A nanoparticle, gold nanoparticle technology, applied in nanotechnology for sensing, nanotechnology for materials and surface science, nanotechnology, etc., can solve problems such as stability decline, and achieve high surface stability, Good stability and not easy to be oxidized

Inactive Publication Date: 2017-05-17
SHANGHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] It has been reported earlier that silver nanoparticles with strong SERS effect are used to detect biomarkers in Paenoid bacillus by SERS. However, in biological systems, the surface

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Operation and characterization of a method for detecting bacillus markers using nanoparticle small aggregates:

[0024] (1) Preparation of 40nm gold particles:

[0025] 2.2 mL of HAuCl 4 The aqueous solution (1%) was mixed with 50 mL of water and heated while stirring until boiling, then 6.5 mL of sodium citrate solution (1%) was added rapidly, and the reduction reaction continued for 15 minutes. At the end of the reaction, the solution was wine red, and it was cooled to room temperature, and the obtained gold nanoparticles were ~40 nm.

[0026] (2) Construction of small aggregates of nanoparticles:

[0027] The cyanine dye solution was added to the nano-sol so that the concentrations of the two were 0.042 micromole and 1.25 nanomole, respectively, so that the dye molecules on the surface of the nanoparticle reached 5-10% coverage. The pH was adjusted to ~3 with hydrochloric acid and stirred well at room temperature for 5 minutes.

[0028] (3) Assembly of 2,6-pyridi...

Embodiment 2

[0031] (1) Preparation of 65nm gold particles:

[0032] 2.2 mL of HAuCl 4 The aqueous solution (1%) was mixed with 50 mL of water and heated while stirring until boiling, then 6.5 mL of sodium citrate solution (1%) was added rapidly, and the reduction reaction continued for 15 minutes. At the end of the reaction, the solution was wine red. According to the particle size, 0.5 mL of Au seeds and 0.5 mL of chloroauric acid aqueous solution (1%) were mixed at room temperature and then 2.1 mL of NH 2 OH·HCl solution (40 mM) was used as a reducing agent to finally obtain ~65 nm gold sol.

[0033] (2) Construction of small aggregates of nanoparticles:

[0034] The cyanine dye solution was added to the nano-sol so that the concentrations of the two were 0.104 micromole and 0.024 nanomole, respectively, so that the dye molecules on the surface of the nanoparticle reached 5-10% coverage. The pH was adjusted to ~3 with hydrochloric acid and stirred well at room temperature for 5 minut...

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Abstract

The invention discloses a method for detecting a bacillus-like marker by utilizing a small polymer of nanoparticles. According to the invention, cyanine dye molecules serve as bridges to pairwise crosslink single nanoparticles to establish the small polymer of the nanoparticles, and then the small polymer of the nanoparticles serves as an enhanced substrate in a SERS (Surface-Enhanced Raman Spectrum) for detecting a specific component (biological marker) in a bacillus-like spore. The small polymer of the nanoparticles according to the invention has a strong hot spot effect, and can detect the specific component in the bacillus-like spore, so as to realize sensitive detection of trace amount of analyte.

Description

technical field [0001] The invention belongs to the technical field of surface-enhanced Raman spectroscopy, and in particular relates to a method for detecting bacillus markers by using nanoparticle small aggregates. Background technique [0002] There have been many reports on the synthesis, characterization and application of different types of nanoparticles, but the controllable synthesis and application of small nanoparticle aggregates is still a very meaningful topic, because small nanoparticle aggregates are important in the development of unique optical, It is of great importance in properties such as electricity and biomolecular recognition. [0003] There have been some reports on the use of dye molecules as Raman signal molecules on the surface of nanoparticles, but there are few reports on the use of dye molecules as bridge molecules to construct small aggregates of nanoparticles. Nanoparticle small aggregates constructed by such methods can be used as enhanced s...

Claims

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Application Information

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IPC IPC(8): G01N21/65B82Y15/00B82Y30/00
CPCG01N21/658B82Y15/00B82Y30/00
Inventor 成汉文陈浩天朱丽莹罗谨
Owner SHANGHAI INST OF TECH
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