SERS biological probe and method for making same

A technology of surface-enhanced Raman and biological probes, which is applied in Raman scattering, biological testing, material inspection products, etc., can solve the problems of detection sensitivity limitation, difficult Raman signal molecules, etc. Mann signal and high detection sensitivity

Inactive Publication Date: 2009-07-29
CAPITAL NORMAL UNIVERSITY
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  • Abstract
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AI Technical Summary

Problems solved by technology

In the current SERS research, most of the Raman signal molecules are directly combined with the substrate composed of spherical gold and silver nanoparticles to enhance the Raman signal [Science 2002, 297, 1536; Anal.Chem.1999, 71, 4903; Chem .Phys.Lett.1981,82,355.], the shortcoming of this method is: (1) must use gold, silver particle to prepare granular film as the detection substrate of SERS; (2) a Raman signal molecule can only be combined in For detection on one molecule, it is difficult to realize multiple Raman signal molecules combined on one molecule for detection at the same time, so the detection sensitivity is greatly limited

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preparation example Construction

[0029] 4) Preparation of SERS bioprobe: SiO coated with gold or silver nanoparticles prepared in step 3) 2 Core-shell particle SiO 2 @nano-Au or SiO 2 On the surface of @nano-Ag, Raman signal molecules are adsorbed, and then a layer of nano-silicon dioxide layer with controllable thickness is formed to form a composite nanoparticle (SiO 2 @nano-Au @SiO 2 or SiO 2 @nano-Ag @SiO 2 ), so that multiple Raman signal molecules can be wrapped in the "sandwich layer" of gold and silver nanoparticles in composite particles; finally, biological probe molecules (DNA probes or antibody molecules) are modified on the surface of composite particles to prepare SERS bioprobes can be used for SERS sensitive detection of biomolecules.

[0030] Commonly used, silicon dioxide (SiO 2 ) particles can be carried out with reference to the literature [J.Colloid Interface Sci.1968,26,62.]; gold and silver nanoparticles of different shapes (spherical, triangular, rod-shaped) can be carried out wit...

Embodiment 1

[0033] Example 1. Taking gold nanorods as an example to prepare novel SERS immunoprobes:

[0034] 1) Prepare gold nanorods (Au rod ); References (J.Colloid Interface Sci.1968, 26, 62.) Preparation of silica nanoparticles.

[0035] 2)SiO2 2 @Au rod Preparation: Disperse 105 mg of the prepared silica particles into 1000 mL of absolute ethanol, add 20 mL of 3-aminopropyl-trimethoxysilane under stirring, and react for 10 minutes to obtain aminated silica particles. Add 30 mL of 1.8 mol / L PVP solution to the prepared gold nanorod particle solution, centrifuge after 20 min, and add it to the aminated SiO 2 After 1 min, the reaction solution was centrifuged, washed with pure water and ethanol, ultrasonically and then centrifuged to remove excess PVP, so that the gold nanorods were fixed on the nanospheres of silicon dioxide.

[0036] 3) Raman signal molecules (such as p-mercaptoaniline, 4-ATP) on SiO 2 @Au rod Fixation on particle surface: add 1.5mol / L 4-ATP solution to SiO 2 ...

Embodiment 2

[0049] Taking triangular silver nanoparticles as an example to prepare a new type of SERS immunoprobe:

[0050] The rest of the preparation process is the same as in Example 1, except that triangular silver nanoparticles are used instead of gold nanorod particles according to literature (Adv. Mater. 2005, 17, 412.).

[0051] SERS detection of human IgG (h-IgG) using the SERS immunoprobe prepared by triangular silver nanoparticles:

[0052] Except utilizing the SERS immunoprobe prepared by triangular silver nanoparticles to replace the SERS immunoprobe prepared by gold nanorod particles, the rest of the detection process is the same as in Example 1, and the results show that the detection limit for human IgG has reached 15pg / mL.

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Abstract

The invention discloses a SERS biological probe and a preparation method thereof. The SERS biological probe provided by the present invention includes a silicon dioxide particle core and a metal nanoparticle layer on the surface of the silicon dioxide particle core, and several Raman signal molecules are connected on the metal nanoparticle layer. A silicon dioxide layer is also formed outside the nanoparticle layer, and the Raman signal molecules are located between the metal nanoparticle layer and the silicon dioxide layer; the surface of the silicon dioxide layer is modified with biological probe molecules; the The metal nanoparticle layer is a gold nanoparticle layer or a silver nanoparticle layer. The SERS biological probe provided by the present invention has wide universality, and will be used in the recognition and detection of biomolecules (DNA molecules, protein molecules), rapid diagnosis of diseases, biomedical imaging technology, and treatment of major diseases, food hygiene, and environmental protection. Monitoring and other fields have broad application prospects.

Description

technical field [0001] The invention relates to a novel surface-enhanced Raman spectroscopy (SERS) biological probe and a preparation method thereof. Background technique [0002] In recent years, laser Raman spectroscopy has become a common spectroscopic method for studying the structure of biomolecules, especially playing an important role in studying the structure and conformation of proteins in aqueous solution. Raman technology has the following main advantages in the study of biological samples: first, it can directly obtain the information of groups and chemical bonds at the molecular level and the influence of the microenvironment on the structure of biological samples; second, it does not destroy the structure of the sample, It can be used in the research of aqueous solution system. Based on these characteristics, it occupies an important position in the study of biological systems. However, the signal intensity of conventional Raman spectroscopy is very low, whic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65G01N33/52
Inventor 马占芳
Owner CAPITAL NORMAL UNIVERSITY
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