Bacillus pumillus microbial preparation with quorum sensing system inhibiting effect

A quorum sensing system, technology of Bacillus pumilus, applied in the field of microbiology

Inactive Publication Date: 2014-01-22
SHANGHAI OCEAN UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on a microbial preparation of Bacillus pumilus with quorum sensing system inhibition

Method used

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  • Bacillus pumillus microbial preparation with quorum sensing system inhibiting effect
  • Bacillus pumillus microbial preparation with quorum sensing system inhibiting effect
  • Bacillus pumillus microbial preparation with quorum sensing system inhibiting effect

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Experimental program
Comparison scheme
Effect test

Embodiment

[0026] 1. Materials and methods

[0027] 1.1 Materials

[0028] 1.1.1 Strains and activation

[0029] Violet Bacillus ( Chromobacteri μm violaceum ) was purchased from ATCC in the United States; Bacillus strains X77, X93, and X3914 were preserved in our laboratory.

[0030] Transfer the Violet Bacillus preserved in lyophilized powder to 5ml of LB liquid medium, and incubate at 26°C for 24 hours; then take 1mL of the bacterial liquid and transfer it to 5mL of LB liquid medium for 24h at 26°C; use an inoculation loop to take the bacterial liquid in Streak and separate from LB plates, pick a single purple colony and streak on LB slant for future use.

[0031] 1.1.2 Media and reagents

[0032] Nutrient agar medium (purchased from Shanghai Dingguo), LB medium (purchased from Shanghai Sangong); bacterial genome extraction kit (purchased from Shanghai Sangong); PCR reagents: Master Mix 2x, universal primers 1492r, 27f, (Shanghai Meiji Biotechnology Company), D2000 marker (purch...

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Abstract

The invention relates to bacillus pumillus with a quorum sensing system inhibiting effect. The preservation number of the bacillus pumillus is CCTCC M2013240. The invention also provides an application of the bacillus pumillus and a microbial preparation which takes the bacillus pumillus or bacillus pumillus fermentation liquid as an active component. The invention has the advantages that chromobacterium violaceum is used as a screening model, a T-shaped streaking method and a filter paper method are used for screening out a bacterial strain F3-1 which can suppress the generation of the purple pigment of the chromobacterium violaceum, test results show that the bacterial strain F3-1 can suppress the QS signal molecule of the chromobacterium violaceum, has the effect of suppressing bacterial colony sensing and can be used for preparing the microbial preparation capable of preventing and treating aquatic bacterial diseases, and the foundation is laid for further researching a new aquatic bacterial disease preventing and treating method from the point of an interference bacterial colony sensing system.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a bacillus pumilus microbial preparation with quorum sensing system inhibitory effect. Background technique [0002] Bacterial diseases have always been one of the important reasons that plague the development of aquaculture. In recent years, while the high-density and intensive production mode has promoted the increase of production in the aquaculture industry, it has also increased the chance, frequency and harm of bacterial diseases; in addition, drug resistance, drug residues and The destruction of the ecological environment has also become the focus of social attention. Therefore, finding alternative drugs to antibiotics and exploring new control methods have become one of the important research directions for aquatic disease control. Quorum sensing (QS for short) means that bacteria can spontaneously produce and release some specific signaling molecules, sense changes in the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/74A61P31/04C12R1/07A61K35/742
Inventor 宋增福范斌陈彪
Owner SHANGHAI OCEAN UNIV
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