Preparation of electrochemical luminescence paper chip and application of chip in hydrogen sulfide detection

A paper chip and electrochemical technology, which is applied in the direction of chemiluminescence/bioluminescence, and analysis through chemical reactions of materials, can solve the problems of wide linear range and unsuitability for clinical online detection, so as to improve the flow rate and reduce volatilization , the effect of improving the sensitivity

Inactive Publication Date: 2015-08-05
UNIV OF JINAN
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Problems solved by technology

These detection methods have high sensitivity and wide l...

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  • Preparation of electrochemical luminescence paper chip and application of chip in hydrogen sulfide detection
  • Preparation of electrochemical luminescence paper chip and application of chip in hydrogen sulfide detection
  • Preparation of electrochemical luminescence paper chip and application of chip in hydrogen sulfide detection

Examples

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Embodiment 1

[0041] Preparation of an electrochemiluminescent hollow channel paper chip and its application in the detection of hydrogen sulfide in cancer cells:

[0042] (1) Design the hydrophobic wax printing pattern of the hollow channel paper chip on the computer. The diameter of the hydrophilic area where the working electrode is located is 6 mm. In the water area, the spacing between four working electrodes is 15 mm. The opposite hydrophilic area corresponding to the elliptical area between the four working electrodes is the sample pretreatment area. The diameter of the major axis of the ellipse is 3.5 mm, and the diameter of the minor axis is 2 mm. mm, the diameter of the hollow channel is exactly the same as that of the left paper chip, the right paper chip is the area where the reference electrode and the counter electrode are located, the diameter is 8 mm, and the middle area is a hole of 2 mm;

[0043] (2) Print the filter paper chip designed in step (1) on a hydrophobic pattern...

Embodiment 2

[0056] The preparation steps are the same as Example 1, except that the paper chip used is chromatographic paper.

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Abstract

The invention discloses a method for preparing an electrochemical luminescence paper chip and a method for measuring content of signal molecule hydrogen sulfide in MCF-7 by using an electrochemical luminescence sensor. The method is characterized by preparing a hydrophobic region, a semi-hydrophobic region, a hydrophilic region and a hollow channel on paper by utilizing a wax printing technology, cutting the hollow channel by virtue of a laser cutting machine, preparing proper printing ink, printing a corresponding reference electrode and a working electrode on the paper, functionalizing the working area, dripping stock solution in a pretreatment region, and fixing cancer cells; and folding the prepared paper chip, forming a three-electrode system, dropwise adding electrolyte solution in a reaction region, connecting an electrochemical workstation, thus realizing high-sensitivity detection of the low-content signal molecules in the cancer cells.

Description

technical field [0001] The invention relates to the technical field of low-cost, simple and portable hydrogen sulfide analysis and detection, and more specifically relates to the construction of an electrochemiluminescence hollow channel paper chip analysis device. Background technique [0002] With the rapid development of economy and culture, people's living standards have been significantly improved, and people's investment in their own health problems is also increasing. Cancer, as one of the five terminal diseases, directly threatens human health. Therefore, the early diagnosis and treatment of cancer has become one of the important research topics for scientific researchers today. At present, there are mainly two ways of clinical diagnosis of cancer. One is to conduct preliminary diagnosis through large-scale instruments and equipment in the hospital. This method is highly accurate, but time-consuming, laborious and expensive; equipment to realize on-site rapid analys...

Claims

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Application Information

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IPC IPC(8): G01N21/76
Inventor 于京华李丽颜梅葛慎光刘海云张彦苏敏刘芳梁琳琳
Owner UNIV OF JINAN
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