Preparation of electrochemical luminescence paper chip and application of chip in hydrogen sulfide detection

A paper chip and electrochemical technology, which is applied in the direction of chemiluminescence/bioluminescence, and analysis through chemical reactions of materials, can solve the problems of wide linear range and unsuitability for clinical online detection, so as to improve the flow rate and reduce volatilization , the effect of improving the sensitivity
CN104819976AInactive Publication Date: 2015-08-05UNIV OF JINAN
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
UNIV OF JINAN
Publication Date
2015-08-05
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for preparing an electrochemical luminescence paper chip and a method for measuring content of signal molecule hydrogen sulfide in MCF-7 by using an electrochemical luminescence sensor. The method is characterized by preparing a hydrophobic region, a semi-hydrophobic region, a hydrophilic region and a hollow channel on paper by utilizing a wax printing technology, cutting the hollow channel by virtue of a laser cutting machine, preparing proper printing ink, printing a corresponding reference electrode and a working electrode on the paper, functionalizing the working area, dripping stock solution in a pretreatment region, and fixing cancer cells; and folding the prepared paper chip, forming a three-electrode system, dropwise adding electrolyte solution in a reaction region, connecting an electrochemical workstation, thus realizing high-sensitivity detection of the low-content signal molecules in the cancer cells.
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Description

technical field

[0001] The invention relates to the technical field of low-cost, simple and portable hydrogen sulfide analysis and detection, and more specifically relates to the construction of an electrochemiluminescence hollow channel paper chip analysis device. Background technique

[0002] With the rapid development of economy and culture, people's living standards have been significantly improved, and people's investment in their own health problems is also increasing. Cancer, as one of the five terminal diseases, directly threatens human health. Therefore, the early diagnosis and treatment of cancer has become one of the important research topics for scientific researchers today. At present, there are mainly two ways of clinical diagnosis of cancer. One is to conduct preliminary diagnosis through large-scale instruments and equipment in the hospital. This method is highly accurate, but time-consuming, laborious and expensive; equipment to realize on-site rapid analys...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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