Preparation of addressable electrochemical transducer array, and application of addressable electrochemical transducer array to detection of multiple tumor markers and cancer screening

A tumor marker and sensor array technology, which is applied in the field of preparation of a variety of tumor markers and addressable electrochemical sensor arrays, can solve the problem of low specificity, difficulty in finding a variety of markers to label signal molecules, and low positive rate. problems, to achieve the effect of broad application prospects

Inactive Publication Date: 2013-03-20
UNIV OF JINAN
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Problems solved by technology

There are deficiencies in the existing tumor marker detection or screening methods: In order to avoid the problems of low positive rate and low specificity of a single tumor marker in the detection, a combination of multiple tumor markers is often used for detection. A method of multivariate analysis to improve the positive rate a

Method used

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  • Preparation of addressable electrochemical transducer array, and application of addressable electrochemical transducer array to detection of multiple tumor markers and cancer screening
  • Preparation of addressable electrochemical transducer array, and application of addressable electrochemical transducer array to detection of multiple tumor markers and cancer screening
  • Preparation of addressable electrochemical transducer array, and application of addressable electrochemical transducer array to detection of multiple tumor markers and cancer screening

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Embodiment Construction

[0031] Preparation of addressable electrochemical sensor array and its application in detection of various tumor markers and screening of cancer

[0032] (1) Using screen printing technology and graphite as raw material, firstly take an equal volume of acetone and cyclohexanone solution, mix and weigh, then weigh the corresponding amount of cellulose acetate and add it to the above solution, and use ultrasonic ultrasound to make the cellulose acetate fully Dissolve in a mixed solvent of acetone and cyclohexanone, finally add a certain amount of graphite powder, mix well with a glass rod, and print row electrodes and column electrodes with a diameter of 5 mm on a polyethylene terephthalate substrate. After being orthogonal, the row electrodes overlap the column electrodes to form a 4×6 electrode array;

[0033] (2) Use Adobe illustrator CS4 to design the pattern, and use the wax printing technology to print the pattern on the first-grade chromatographic paper through the printe...

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Abstract

The invention discloses a method for preparation of an addressable electrochemical transducer array, and determination multiple tumor markers in a sample by adopting the addressable electrochemical transducer array. The method comprises the following steps: printing a row electrode and a line electrode on a proper substrate material by preparing proper ink; selecting a proper paper material, preparing a reaction hydrophobic region and a hydrophilic region on the paper by utilizing a wax printing technology, and constructing a reference electrode and a counter electrode shared by an array electrode on the other paper-based material through a screen printing technology; functionalizing the prepared hydrophilic region, identifying an antigen, and marking a signal molecule; assembling the prepared working electrode, the reference electrode and the counter electrode with the prepared reaction region, and constructing the addressable electrochemical transducer array; and dripping an enzyme substrate in the reaction region, and adding phosphoric acid buffer liquid on the paper layer printed with the reference electrode and the counter electrode, and detecting the target.

Description

technical field [0001] The present invention relates to the technical field of tumor marker detection, more specifically to the preparation of an addressable electrochemical sensor array, and the present invention also relates to a method for using the addressable electrochemical sensor to measure multiple tumor markers in a sample . Background technique [0002] Cancer is one of the diseases with high morbidity and mortality that seriously threatens human health. However, if cancer can be diagnosed at an early stage of development, the survival rate of patients will be greatly improved. It can be said that early diagnosis and early treatment are one of the most effective ways to prevent cancer development and reduce mortality. Tumor markers are a class of substances that are produced, secreted, and released into blood, cells, and body fluids by tumor cells during tumorigenesis and proliferation, reflecting the existence and growth of tumors. It is a specific representatio...

Claims

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Application Information

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IPC IPC(8): G01N27/26
Inventor 葛慎光于京华颜梅葛磊黄加栋张彦李伟平李蒙王衍虎李龙苏敏
Owner UNIV OF JINAN
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