Preparation of addressable electrochemical transducer array, and application of addressable electrochemical transducer array to detection of multiple tumor markers and cancer screening

Abstract

The invention discloses a method for preparation of an addressable electrochemical transducer array, and determination multiple tumor markers in a sample by adopting the addressable electrochemical transducer array. The method comprises the following steps: printing a row electrode and a line electrode on a proper substrate material by preparing proper ink; selecting a proper paper material, preparing a reaction hydrophobic region and a hydrophilic region on the paper by utilizing a wax printing technology, and constructing a reference electrode and a counter electrode shared by an array electrode on the other paper-based material through a screen printing technology; functionalizing the prepared hydrophilic region, identifying an antigen, and marking a signal molecule; assembling the prepared working electrode, the reference electrode and the counter electrode with the prepared reaction region, and constructing the addressable electrochemical transducer array; and dripping an enzyme substrate in the reaction region, and adding phosphoric acid buffer liquid on the paper layer printed with the reference electrode and the counter electrode, and detecting the target.

Description

technical field [0001] The present invention relates to the technical field of tumor marker detection, more specifically to the preparation of an addressable electrochemical sensor array, and the present invention also relates to a method for using the addressable electrochemical sensor to measure multiple tumor markers in a sample . Background technique [0002] Cancer is one of the diseases with high morbidity and mortality that seriously threatens human health. However, if cancer can be diagnosed at an early stage of development, the survival rate of patients will be greatly improved. It can be said that early diagnosis and early treatment are one of the most effective ways to prevent cancer development and reduce mortality. Tumor markers are a class of substances that are produced, secreted, and released into blood, cells, and body fluids by tumor cells during tumorigenesis and proliferation, reflecting the existence and growth of tumors. It is a specific representatio...

AI Technical Summary

Problems solved by technology

There are deficiencies in the existing tumor marker detection or screening methods: In order to avoid the problems of low positive rate and low specificity of a single tumor marker in the detection, a combination of multiple tumor markers is often used for detection. A method of multivariate analysis to improve the positive rate and specificity of tumor diagnosis

For the detection of multiple tumor markers, the multi-signal labeling mode is generally used. This mode needs to detect the signals of different markers at the same time, so there are usually certain difficulties in accurate quantification. Available labeled signaling molecules for various markers

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