Method for preparing yolk immune globulin nanometer liposome for preventing and controlling infectious bursal disease

A yolk immunoglobulin and nanoliposome technology, which is applied in the directions of liposome delivery, antibodies, antiviral agents, etc., can solve the problems of short action time and short half-life, and achieve stable product performance, improved curative effect, economical and practical Effect

Inactive Publication Date: 2011-10-12
张文生
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AI Technical Summary

Problems solved by technology

This immunoglobulin is destroyed quickly in the body and has a short half-life and short duration of action

Method used

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  • Method for preparing yolk immune globulin nanometer liposome for preventing and controlling infectious bursal disease
  • Method for preparing yolk immune globulin nanometer liposome for preventing and controlling infectious bursal disease
  • Method for preparing yolk immune globulin nanometer liposome for preventing and controlling infectious bursal disease

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Embodiment 1

[0026] See attached figure 1 .

[0027] The preparation method of the present invention for preventing and treating chicken infectious bursal disease yolk immunoglobulin nano-liposome comprises the following process steps:

[0028] 1. Preparation of egg yolk antibody: The experimental chickens were vaccinated by multiple and alternating immunization schemes. When the detection titer of the egg yolk antibody AGPT of the experimental chicken is above 1:64, the egg yolk is collected.

[0029] 2. Separation of immunoglobulins: Dilute the egg yolk suspension 9 times with acetic acid-sodium acetate buffer solution with pH 5.0, stir, stand still, centrifuge, and retain the supernatant. The supernatant was concentrated.

[0030] 3. Preparation of liposomes: a thin film dispersion method is used to make a liposome film; yolk immunoglobulin is added through rotation and stirring to form yolk immunoglobulin liposome capsules. The formed liposomes are then restructured with a high-spe...

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Abstract

The invention relates to a method for preparing a yolk immune globulin nanometer liposome for preventing and controlling infectious bursal disease, and belongs to the technical field of medicine preparations of animals. The method for preparing the yolk immune globulin nanometer liposome for preventing and controlling chicken infectious bursa of Fabricius comprises the following steps of: 1, preparing a yolk antibody, namely performing vaccine inoculation on chickens by a multi-time and alternate immune method, and when the agar gel precipitate test (AGPT) detection valence of the yolk antibody is over 1:64, collecting yolk; 2, separating immune globulin, namely diluting yolk suspension by using acetic acid-sodium acetate buffer solution, stirring, standing, centrifuging and reserving supernate; and 3, preparing a liposome, namely preparing a liposome membrane by a membrane dispersion method, adding the yolk immune globulin, rotating and stirring to form a yolk immune globulin liposome capsule, and reforming the formed liposome capsule by using a homogenizer to obtain the nanometer liposome. In the method, the yolk immune globulin is enveloped, and the method has the advantages of simple process, economy, practicability, stable economic performance, good treatment effect and the like.

Description

technical field [0001] The invention belongs to the technical field of oral products, in particular to a method for preparing egg yolk immunoglobulin nano-liposomes for preventing and treating infectious bursal disease of chickens. Background technique [0002] At present, chicken infectious bursal disease (Infectious bursal disease, IBD) is a kind of viral infectious disease caused by a double-stranded RNA virus that destroys the bursa of the immune center organ of chickens. On the one hand, due to the increase in chicken mortality and elimination rate, the disease affects the weight gain of chickens and causes direct economic losses; bronchitis live vaccine, chicken Marek's disease live vaccine, etc.), the immune response ability is reduced or there is no immune response. At the same time, due to the decline of the body's immune function, the sick chickens are more susceptible to pathogens, and are prone to concurrent or secondary multiple other diseases, such as colibaci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/42A61K9/127A61P31/14
Inventor 张文生李富桂赵云英常蕊张燕欣
Owner 张文生
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