Micro-ecological preparation and application thereof in improvement of production performance, immune performance as well as chicken quality of broiler

Abstract

The invention discloses a micro-ecological preparation and an application thereof in improvement of production performance, immune performance as well as chicken quality of broiler. The micro-ecological preparation is characterized in that a culture obtained by culturing a complex microbial inoculant through a fermentation medium is used as an active ingredient; the active ingredient of the complex microbial inoculant comprises bacillus subtilis, saccharomyces cerevisiae and lactobacillus plantarum. The micro-ecological preparation can obviously increase the content of valine in chicken, the content of methionine, the content of lysine, the content of phenylalanine, the content of cystine, the content of tyrosine, the content of proline and the content of crude protein, improve the tenderness and elasticity of pectoral muscles and the chewiness of chicken thigh, decrease the content of cholesterol of chicken, the content of fat, and drip loss, and can obviously increase the crop rate of broiler, meat-to-feed rate, protein, apparent digestibility of calcium and phosphorus, body weight, thymus index, spleen index, bursa of Fabricius index, erythrocyte C3b receptor rosette rate, red blood cell-immune complex receptor rosette rate, the content of protein in serum, the content of alpha-amylase, and the content of cholinesterase.

Description

technical field

[0001] The invention relates to a microecological preparation and its application in improving broiler production performance, immune performance and chicken quality. Background technique

[0002] During the long-term evolution of broilers, the microorganisms in the digestive tract and the host have formed a stable symbiotic relationship, which can maintain the micro-ecological balance in broilers, and has the functions of promoting the development of digestive organs, resisting the invasion of pathogenic microorganisms, and buffering the sudden impact of unfavorable factors. It is beneficial to maintain good health level and production performance of broilers. In recent years, the scale of broiler farming has gradually expanded. Although the scale of industrialized farming has brought greater economic benefits to farmers, the adverse factors such as the deterioration of the breeding environment, the abuse of antibiotics and other preventive drugs, and the pr...

Examples

Embodiment 1

[0075] Embodiment 1, the preparation of composite bacterial agent for broilers

[0076] This example relates to two kinds of composite bacterial agents for broilers, which are composite bacterial agents A for broilers and composite bacterial agents B for broilers.

[0077] Composite microbial agent A for broilers is to dilute the liquid strains of Bacillus subtilis RJGP16, Saccharomyces cerevisiae CICC1562 and Lactobacillus plantarum CICC22696 to 10 8 After cfu / ml, the resulting mixture was mixed according to the colony-forming unit number ratio of 1:3:1.

[0078] The compound bacterial agent B for broilers is to dilute the liquid strains of Bacillus subtilis RJGP16, Saccharomyces cerevisiae CICC1562 and Lactobacillus plantarum CICC22696 to 10% respectively with sterile water. 8 After cfu / ml, the resulting mixture was mixed according to the colony-forming unit number ratio of 1:1:1.

[0079] Wherein, the liquid strain of Bacillus subtilis RJGP16 is a culture obtained by cult...

Embodiment 2

[0083] Embodiment 2, the preparation of broiler probiotics

[0084] This example provides two kinds of probiotics for broiler chickens, respectively called probiotics A for broilers and probiotics B for broilers.

[0085] The probiotics A for broilers is a culture obtained by using the fermentation medium A of Example 1 to cultivate the composite microbial agent A for broilers of Example 1. The specific preparation method is as follows: the broiler composite bacterial agent A of embodiment 1 is inserted into the 200L embodiment according to the inoculum amount (the volume of the composite bacterial agent inserted and the volume ratio of the fermentation medium) of 6% (volume ratio). In the 500L fermenter of the fermentation medium A of 1, cultivate for 42 hours at 34°C, with an aeration rate of 600L / h and a stirring speed of 150rpm, collect all the cultured substances (fermentation liquid) in the fermenter, and add The rice husk powder was dried at 50° C. to obtain the probio...

Embodiment 3

[0087] Embodiment 3, improve broiler body weight with the broiler chicken probiotic A of embodiment 2

[0088] 1.1 Test animals

[0089] Select 12,000 1-day-old healthy chicks of Arboriga—Quick large white-feathered broiler chicken with a body weight of 50±5 grams, and divide them into 4 treatment groups at random, namely, the control group, the 0.015% probiotics group, and the 0.025% probiotics group. Ecological preparation group and 0.05% probiotic preparation group. There were 3 replicates per treatment group with 1000 chickens per replicate.

[0090] 1.2 Diet

[0091] Broiler brooding period diets were divided into broiler brooding period common rations, 0.015% broiler probiotics A brooding rations, 0.025% broiler probiotics A brooding rations, 0.05% broiler probiotics A brooding rations. Among them, the common diet for broiler brooding period is shown in Table 1; 0.015% broiler chicken probiotics A brooding period diet is added to the common diet for broiler chicken br...

technical field

[0001] The invention relates to a microecological preparation and its application in improving broiler production performance, immune performance and chicken quality. Background technique

[0002] During the long-term evolution of broilers, the microorganisms in the digestive tract and the host have formed a stable symbiotic relationship, which can maintain the micro-ecological balance in broilers, and has the functions of promoting the development of digestive organs, resisting the invasion of pathogenic microorganisms, and buffering the sudden impact of unfavorable factors. It is beneficial to maintain good health level and production performance of broilers. In recent years, the scale of broiler farming has gradually expanded. Although the scale of industrialized farming has brought greater economic benefits to farmers, the adverse factors such as the deterioration of the breeding environment, the abuse of antibiotics and other preventive drugs, and the pr...

Examples

Embodiment 1

[0075] Embodiment 1, the preparation of composite bacterial agent for broilers

[0076] This example relates to two kinds of composite bacterial agents for broilers, which are composite bacterial agents A for broilers and composite bacterial agents B for broilers.

[0077] Composite microbial agent A for broilers is to dilute the liquid strains of Bacillus subtilis RJGP16, Saccharomyces cerevisiae CICC1562 and Lactobacillus plantarum CICC22696 to 10 8 After cfu / ml, the resulting mixture was mixed according to the colony-forming unit number ratio of 1:3:1.

[0078] The compound bacterial agent B for broilers is to dilute the liquid strains of Bacillus subtilis RJGP16, Saccharomyces cerevisiae CICC1562 and Lactobacillus plantarum CICC22696 to 10% respectively with sterile water. 8 After cfu / ml, the resulting mixture was mixed according to the colony-forming unit number ratio of 1:1:1.

[0079] Wherein, the liquid strain of Bacillus subtilis RJGP16 is a culture obtained by cult...

Embodiment 2

[0083] Embodiment 2, the preparation of broiler probiotics

[0084] This example provides two kinds of probiotics for broiler chickens, respectively called probiotics A for broilers and probiotics B for broilers.

[0085] The probiotics A for broilers is a culture obtained by using the fermentation medium A of Example 1 to cultivate the composite microbial agent A for broilers of Example 1. The specific preparation method is as follows: the broiler composite bacterial agent A of embodiment 1 is inserted into the 200L embodiment according to the inoculum amount (the volume of the composite bacterial agent inserted and the volume ratio of the fermentation medium) of 6% (volume ratio). In the 500L fermenter of the fermentation medium A of 1, cultivate for 42 hours at 34°C, with an aeration rate of 600L / h and a stirring speed of 150rpm, collect all the cultured substances (fermentation liquid) in the fermenter, and add The rice husk powder was dried at 50° C. to obtain the probio...

Embodiment 3

[0087] Embodiment 3, improve broiler body weight with the broiler chicken probiotic A of embodiment 2

[0088] 1.1 Test animals

[0089] Select 12,000 1-day-old healthy chicks of Arboriga—Quick large white-feathered broiler chicken with a body weight of 50±5 grams, and divide them into 4 treatment groups at random, namely, the control group, the 0.015% probiotics group, and the 0.025% probiotics group. Ecological preparation group and 0.05% probiotic preparation group. There were 3 replicates per treatment group with 1000 chickens per replicate.

[0090] 1.2 Diet

[0091] Broiler brooding period diets were divided into broiler brooding period common rations, 0.015% broiler probiotics A brooding rations, 0.025% broiler probiotics A brooding rations, 0.05% broiler probiotics A brooding rations. Among them, the common diet for broiler brooding period is shown in Table 1; 0.015% broiler chicken probiotics A brooding period diet is added to the common diet for broiler chicken br...

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