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Method for synthesizing bursa of Fabricius bursin

The technology of a bursalin and a synthetic method, which is applied in the field of synthesis of biological preparations, can solve the problems of low production cost, small amount of production, and difficulty in producing bursalin, and achieve reduction in production cost, increase in yield, and significant The effect of applying value

Inactive Publication Date: 2005-11-16
四川斯特佳生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the problem that traditional methods are difficult to produce bursalin and the amount of production is small, the purpose of the present invention is to provide a method for synthesizing bursalin tripeptide with large production capacity and low production cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1), amino acid protection

[0030] a. Dissolve 13.1 g of lysine and 13.9 g of histidine in 50 ml of 2mol·1 -1 In NaOH, ice-salt bath, under stirring, add 34.1 g of Z-Cl pre-dissolved in THF dropwise, and at the same time add 2mol·1 -1 NaOH keeps the solution at pH=9. After the addition is completed, the temperature is naturally raised to room temperature, and the stirring is continued for 1 hour, washed twice with 50 ml of diethyl ether, and the ether layer is separated, and the water phase is added dropwise with 6 mol·1 -1 Hydrochloric acid to pH = 1, add 75 ml of ethyl acetate, continue to stir for 5 minutes, separate the layers, wash the ethyl acetate layer with 5% NaCl until neutral, anhydrous NaCl 2 SO 4 After drying, the solvent was distilled off under reduced pressure to obtain Z 2 -Lys and Z 2 -His;

[0031] b. Dissolve 5.9 g of glycine in 50 ml of methanol, put it in an ice-salt bath and cool to -10°C, slowly add 20 ml of thionyl chloride dropwise, then add...

Embodiment 2

[0046] (1), amino acid protection

[0047] a. Dissolve 13.1 grams of lysine and 13.9 grams of histidine in 50 milliliters of 2mol·l -1 In NaOH, ice-salt bath, add 22 g (Boc) dissolved in THF dropwise under stirring 2 O, while adding 2mol·l dropwise -1 NaOH kept the solution at pH=9. After the addition, it was naturally raised to room temperature, continued to stir for 1 hour, washed twice with 50 ml of ether, and the ether layer was separated, and the aqueous phase was added dropwise with 6 mol l -1 Hydrochloric acid to pH = 1, add 75 ml of ethyl acetate, continue to stir for 8 minutes, separate the layers, wash the ethyl acetate layer with 5% NaCl until neutral, anhydrous NaCl 2 SO 4 After drying, the solvent was distilled off under reduced pressure to obtain Z 2 -Lys and Z 2 -His;

[0048] b. Dissolve 5.9 g of glycine in 50 ml of methanol, put it in an ice-salt bath and cool to -10°C, slowly add 20 ml of thionyl chloride dropwise, then add 10 ml of 98% concentrated su...

Embodiment 3

[0063] (1), amino acid protection

[0064] a. Dissolve 13.1 grams of lysine and 13.9 grams of histidine in 50 milliliters of 2mol·l -1 In NaOH, ice-salt bath, add 22 g (Boc) dissolved in THF dropwise under stirring 2 O, while adding 2mol·l dropwise -1 NaOH keeps the solution at pH = 9. After the addition is complete, let it rise to room temperature naturally, continue to stir for 1 hour, wash twice with 50 ml of ether, separate the ether layer, and add 6 mol l of the aqueous phase dropwise under stirring. -1 Hydrochloric acid to pH = 1, add 75 ml of ethyl acetate, continue to stir for 6 minutes, separate the layers, wash the ethyl acetate layer with 5% NaCl until neutral, anhydrous NaCl 2 SO 4 Drying, removal of solvent by distillation under reduced pressure, to obtain Boc 2 -Lys and Boc 2 -His;

[0065] b. Dissolve 5.9 g of glycine in 50 ml of methanol, put it in an ice-salt bath and cool to -10°C, slowly add 20 ml of thionyl chloride dropwise, then add 10 ml of 98% con...

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Abstract

A process for synthesizing the high-purity bursin tripeptide inclues such steps as amino acid protection, synthesizing the protected His-Gly methyl bipeptide, removing amino acid protection of the protected His-Gly bipeptide, synthesizing the protected lys-His-Gly, removing amino acid protection of the protected His-Gly bipeptide, synthesizing the protected lys-His-Gly, removing amino acid protection, synthesizing bursin tripeptide, and purifying.

Description

technical field [0001] The invention relates to a synthesis method of biological preparations, in particular to a synthesis method of bursalin tripeptide. Background technique [0002] The occurrence of viral infectious diseases is an important factor affecting the development of animal husbandry. After most pathogenic microorganisms invade the body, they can cause damage to the immune organs, including bursal factor, which will weaken the body's immune function, weaken the disease resistance, and make the epidemic further Development, at the same time, lead to secondary infection and immune failure, causing huge losses to the aquaculture industry. Practice has proved that it is difficult to rely solely on vaccine immunity due to various reasons for immune failure on the one hand, and on the other hand due to the limitation of the protection rate of the vaccine itself. Completely prevent the occurrence of infectious diseases, therefore, to seek immun...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K5/08
Inventor 曹冶李键靳亚平李清寒张红赵素君李红周洪群
Owner 四川斯特佳生物科技有限公司
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