Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

New method for evaluating Dorking resistance

A resistance, broiler technology with applications in the fields of molecular genetics, immunology and cell biology that addresses the lack of comprehensiveness

Inactive Publication Date: 2010-10-13
YANGZHOU UNIV
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing methods are only one-sided, analyzing a single disease resistance index, and lack comprehensiveness. By consulting a large amount of data and verifying some related routine experiments, we have selected 6 main disease resistance indexes to complement each other, in order to To achieve a comprehensive and accurate purpose

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • New method for evaluating Dorking resistance
  • New method for evaluating Dorking resistance
  • New method for evaluating Dorking resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The concrete measuring method of 6 resistance indexes that embodiment 1 the present invention chooses:

[0019] 1. Determination of lymphocyte transformation rate

[0020] Take 0.1ml of sterile heparin anticoagulant blood, add 1.8ml of cell culture medium (pre-adjusted to contain 10% calf serum, penicillin 100u / ml, streptomycin μg / ml, adjust pH to 7.2-7.4 with NaHCO3) At the same time, add PHA (1000 μg / ml with RPMI1 640 basal culture medium) 0.1ml, the control tube does not add PHA, the cells are placed at 37 ° C, 5% CO2 Culture for 3 days, shaking once a day. After centrifugation at 2500r / min for 10min, discard the supernatant, leave 0.2ml of pelleted cells to make slices, and dry them quickly. Giemsa dyeing for 10-20min, washed with water, and dried. Since there are more large cells in the upper layer after centrifugation, only the upper layer cells are sucked and counted, and the result is likely to be high. Therefore, the accurate counting method is to pour out t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for evaluating Dorking resistance, which comprises the following steps of: measuring the following indexes: lymphocyte conversion rate; the number of monoclonal antibody-labeled CD4+, CD8+ and T cells and a ratio of the CD4+ to the CD8+; IgG and IgM; an immunocyte index, namely absolute weight of thymus, spleen, and bursa of Fabricius, and the chicken body weight; a blood index, namely red blood cell, and total number of white blood cells, T cells and B cells in per milliliter of blood; and a cell factor index, namely interferon at 7 growth stages every two weeks from 0 to 12 weeks; and performing comprehensive evaluation on the six indexes, and using a comprehensive score as a basis for judging the degree of sample resistance. Compared with the prior art, the method for evaluating the Dorking resistance has the advantages of comprehensiveness and accuracy of Dorking resistance evaluation and wide application prospects.

Description

technical field [0001] The invention relates to the fields of molecular genetics, immunology and cell biology, in particular to a method for evaluating broiler chicken resistance. Background technique [0002] The occurrence of various poultry diseases is unavoidable, and it is on the rise year by year. There are already many methods for broiler resistance evaluation, but they are not standardized and standardized, and it is difficult to reach a consensus. The most important thing is that a single indicator cannot fully explain the problem. Moreover, some resistance indicators measured by different people at different times and in different places with different methods are also difficult to compare and refer to, making it difficult to draw conclusions, which further affects the The development of genetic breeding work and the development of poultry industry. Domestic research on standardizing the operation process of broiler resistance index determination has not been rep...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/06G01N15/10G01N33/50G01N33/53
Inventor 陈国宏刘艳胡国顺栾德琴龚琳琳陈蓉李碧春王伟侯庆文
Owner YANGZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com