Method for preparing avian infectiousness bursa of fabricius virus transfer resistant factor

A chicken infectivity and transfer factor technology, which is applied in the preparation methods of peptides, antiviral agents, chemical instruments and methods, etc., can solve the problems of large loss of product activity, incomplete cell fragmentation, and difficulty in improving yield, etc. Reduced morbidity, no adverse reactions, and easy-to-source effects

Inactive Publication Date: 2008-10-29
TIANJIN SHENGJI GRP CO LTD
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The content of the above patent application is to directly filter and extract the broken tissue after freezing and centrifuging, which also has the defect of incomplete cell crushing, and the process is cumbersome, the time is long, and the activity loss of the product is also large. Therefore, the yi

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Preparation of Anti-Chicken Infectious Bursal Virus Transfer Factor

[0029] 1. Preparation of virus antigen: inoculate chicken infectious bursal virus into the allantoic fluid of 10-day-old chicken embryos, 48-72 hours at 33°C, take the allantoic fluid of chicken embryos, put it in a small thick-walled bottle, and crush it by ultrasonic , centrifuge at 5000rpm for 20 minutes, discard the sediment and leave the supernatant;

[0030] 2. Take the centrifuged supernatant, then discontinuous gradient density centrifugation and purification to extract the viral antigen; after the viral antigen is centrifuged, take the supernatant and fill it with discontinuous gradient density prepared with 15%, 30%, 45% and 60% sucrose Centrifuge tube, ultracentrifuge, 165,000 rpm for 3 hours, determine the position of the centrifuge tube where the virus is located according to the molecular weight of different viruses, and suck it out for later use;

[0031] 3. Immune pigs with the above-...

Embodiment 2

[0042] Preparation of Anti-Chicken Infectious Bursal Virus Transfer Factor

[0043] (1) Preparation of raw materials: 10 healthy 6-month-old pigs with no medical history were selected and immunized with chicken infectious bursal virus vaccine subcutaneously. After 15 days, they were immunized again. The method was the same as above. Slaughter 15-20 days after the second immunization, take the spleen and thymus on ice, transfer and store at -20°C for later use;

[0044] (2) Pretreatment: After weighing the pig spleen, wash the pig spleen with cold three-distilled water, cut off its fascia and adipose tissue with scissors, and then wash it with cold three-distilled water;

[0045] (3) Crushing: Cut the pig spleen washed above into pieces, add 2 times the volume of cold normal saline, and mash it with a high-speed tissue masher (1000r / min) for 3 times under freezing conditions, 3 minutes each time, to prepare get homogenate;

[0046] (4) Freezing and thawing: place the homogena...

Embodiment 3

[0053] Detection of the anti-chicken infectious bursal virus transfer factor prepared by the process of the present invention

[0054] The anti-chicken infectious bursal virus transfer factor (hereinafter referred to as "this product") prepared by the process described in Example 1 is detected as follows:

[0055] (1) Ultraviolet spectrophotometric measurement: This product has a high absorption peak at 250.0-252.0nm, and ABS260 / ABS280>2.0.

[0056] (2) The standard solution is light yellow and the pH value is between 6.0-6.5.

[0057] (3) 20% sulfosalicylic acid test: This product has no turbidity and precipitation, indicating that the protein reaction is negative, and it does not contain macromolecular proteins.

[0058] (4) Determination of peptide content: The peptide content of this product is 1.460mg / ml as determined by the biuret method.

[0059] (5) Determination of nucleic acid content: The nucleic acid content of this product was determined by the orcinol method to...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a preparation method of a natural high-effective biological active substance anti-avian infectious bursal disease virus (IBDV) transfer factor. The preparation method comprises the following steps of: inoculating IBDV into an allantoic fluid obtained from a 10-day-aged chick embryo, and preparing IBDV antigen; extracting the IBDV antigen; immunizing a pig with the extracted IBDV antigen; and extracting the anti-IBDV transfer factor from the immunized pig. The inventive anti-IBDV transfer factor can prevent IBDV infection and protect normal body cells from being infected by the IBDV, so as to reduce incidence rate.

Description

technical field [0001] The invention relates to the field of medicines and preparations thereof, in particular to a preparation method of anti-chicken infectious bursal virus transfer factor. Background technique [0002] Chicken infectious bursal disease, also known as chicken infectious supracavitary bursal disease, is an acute, contagious disease caused by infectious bursal virus. It is characterized by inflammation, necrosis, atrophy, and severe damage of lymphocytes in the bursa of Fabricius. Thereby causing immune dysfunction in chickens and interfering with the immune effects of various vaccines. The morbidity rate is high, almost 100%, and the mortality rate is low, generally 5% to 15%. It is one of the most important diseases in the poultry industry. Under natural conditions, the disease only infects chickens, and all breeds of chickens can be infected. However, among different breeds of chickens, white Leghorns are more sensitive than heavy breeds, and broiler he...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07K1/14C07K1/34A61P31/14
Inventor 田杏芳王连民
Owner TIANJIN SHENGJI GRP CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap