Process for producing small RNA recombinant birds adeno-associated virus for expression of infectivity resistant bursa of fabricius disease virus

A technology of RNA recombination and bursal disease, applied in the field of genetic engineering and biological product research
CN101402945AInactive Publication Date: 2009-04-08YANGZHOU UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
YANGZHOU UNIV
Publication Date
2009-04-08
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a preparation method of establishing a reconstructed aviadenovirus for expressing infectious bursal disease virus (IBDV) miRNA, pertaining to the research field of gene engineering and biological product, comprising the selection of siRNA, the synthesis and cloning of double-stranded short-barrette oligonucleotide, the construction of virus transfer vector containing miRNA expression cassette aviadenovirus, the generation and identification of reconstructed virus expressed, the detection of the duplication function by miRNA to IBDV expressed by the reconstructed virus and the inhibition function to genetic expression. Compared with the prior art, the method can not only effectively and persistently express miRNA in bird cells, has specific and persistent inhibition to the duplication of virus and expression of gene of IBDV, but also has not infective function to poultry, which can be applied to in vitro experiments as well as be developed as a novel anti-avain-virus biological product.
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Description

Technical field:

[0001] The invention belongs to the field of genetic engineering and biological product research. In particular, it relates to a preparation method for expressing anti-infectious bursal disease virus microRNA recombined avian adeno-associated virus. Background technique:

[0002] RNA interference (RNA interference, RNAi) is a sequence-specific post-transcriptional gene silencing technique that has developed rapidly in recent years, and has a wide range of applications in functional genomics research, new drug development, cancer treatment, and antiviral infection. In terms of antiviral infection, existing research data show that RNAi can almost inhibit the replication of all viruses (including DNA and RNA viruses) to varying degrees.

[0003] Interfering RNAs with RNAi effects include short interfering RNAs (siRNAs) and microRNAs (miRNAs). Currently, the methods for obtaining siRNA or miRNA mainly include chemical synthesis, in vitro transcription and vect...

Claims

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