Novel muscovy duck reovirus disease vaccine and application thereof

A technology of reovirus and duck reo, applied in the direction of antiviral agent, virus antigen component, etc., to achieve the effect of strong practicability

Inactive Publication Date: 2012-08-15
INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
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Problems solved by technology

The Institute of Animal Husbandry and Veterinary Medicine of Fujian Academy of Agricultural Sciences confirmed for the first time that the pathogen of duck hemorrhagic necrotizing hepatitis is different from Muscovy duck reovirus and fowl reovirus from the aspect

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Embodiment Construction

[0006] The present invention will be further described below in conjunction with specific examples. (but not a limitation of the invention).

[0007] Firstly, the duck reovirus NDRV-JM85 strain was used to inoculate 11-day-old muscovy duck embryo multiplication virus through the allantoic cavity, and the toxic allantoic fluid was harvested; or a single layer of muscovy duck embryo fibroblasts was inoculated, when the cytopathy reached 75% Harvest the cytotoxicity; After the above-mentioned virus is properly concentrated and inactivated by formaldehyde, it is mixed with white oil adjuvant in an appropriate proportion to make a new type of duck reovirus disease inactivated vaccine, and then the above-mentioned inactivated vaccine is used to prevent new Duck reovirus disease; breeding duck immunity and neutralizing antibody: take the inactivated vaccine to immunize breeding ducks once, 10ml / feather; booster immunization once 4 weeks later, 10ml / feather. The duck reovirus NDRV-JM...

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Abstract

The invention discloses a novel muscovy duck reovirus disease vaccine and an application of the novel muscovy duck reovirus disease vaccine. Firstly, a novel muscovy duck reovirus disease (NDRV)-JM85 plant is inoculated with a 11-day muscovy duck embryo proliferation virus by an allantoic cavity to obtain poison-containing allantoic fluid, or the NDRV-JM85 plant is inoculated with a muscovy duck embryo fibroblast cell monolayer; when cytopathy is above 75%, the cellular poison is harvested; after the virus is properly condensed and is inactivated by formaldehyde, the virus and white oil adjuvant are evenly mixed at a proper ratio to obtain the novel muscovy duck reovirus disease vaccine; and then the novel muscovy duck reovirus disease vaccine is used for preventing the novel muscovy duck reovirus disease. The inactivated vaccine is used for immunizing the breeding duck once, and each feather is applied with 1.0ml, immunization is boosted once in four weeks, and each feather is applied with 1.0ml.

Description

technical field [0001] The invention relates to a novel duck reovirus disease vaccine and its application in preventing the new duck reovirus disease, belonging to the field of preventive veterinary medicine. Background technique [0002] Since 2005, an epidemic disease characterized by irregular liver necrosis and mixed hemorrhage has occurred in duck populations such as Muscovy ducks, semi-muscle ducks, and shelducks in Fujian, Guangdong, and Zhejiang provinces in China, because the main lesion is hepatic hemorrhagic clots / spots and necrotic spots / focus, commonly known as "duck hemorrhagic necrotizing hepatitis". The disease has no obvious seasonality, and all kinds of ducks (such as Muscovy duck, semi-muscovy duck, Shelduck duck, Peking duck, etc.) can occur, and there is a trend of increasing year by year; the age of onset is about 3 to 25 days old, of which Most of them are 5 to 10 days old, the course of disease is 5 to 7 days, the incidence rate is 5% to 20%, and...

Claims

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Application Information

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IPC IPC(8): A61K39/15A61P31/14
Inventor 陈仕龙陈少莺林锋强程晓霞朱小丽王劭李兆龙黄梅清
Owner INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
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