Novel muscovy duck reovirus disease vaccine and application thereof

Abstract

The invention discloses a novel muscovy duck reovirus disease vaccine and an application of the novel muscovy duck reovirus disease vaccine. Firstly, a novel muscovy duck reovirus disease (NDRV)-JM85 plant is inoculated with a 11-day muscovy duck embryo proliferation virus by an allantoic cavity to obtain poison-containing allantoic fluid, or the NDRV-JM85 plant is inoculated with a muscovy duck embryo fibroblast cell monolayer; when cytopathy is above 75%, the cellular poison is harvested; after the virus is properly condensed and is inactivated by formaldehyde, the virus and white oil adjuvant are evenly mixed at a proper ratio to obtain the novel muscovy duck reovirus disease vaccine; and then the novel muscovy duck reovirus disease vaccine is used for preventing the novel muscovy duck reovirus disease. The inactivated vaccine is used for immunizing the breeding duck once, and each feather is applied with 1.0ml, immunization is boosted once in four weeks, and each feather is applied with 1.0ml.

Description

technical field [0001] The invention relates to a novel duck reovirus disease vaccine and its application in preventing the new duck reovirus disease, belonging to the field of preventive veterinary medicine. Background technique [0002] Since 2005, an epidemic disease characterized by irregular liver necrosis and mixed hemorrhage has occurred in duck populations such as Muscovy ducks, semi-muscle ducks, and shelducks in Fujian, Guangdong, and Zhejiang provinces in China, because the main lesion is hepatic hemorrhagic clots / spots and necrotic spots / focus, commonly known as "duck hemorrhagic necrotizing hepatitis". The disease has no obvious seasonality, and all kinds of ducks (such as Muscovy duck, semi-muscovy duck, Shelduck duck, Peking duck, etc.) can occur, and there is a trend of increasing year by year; the age of onset is about 3 to 25 days old, of which Most of them are 5 to 10 days old, the course of disease is 5 to 7 days, the incidence rate is 5% to 20%, and...

AI Technical Summary

Problems solved by technology

The Institute of Animal Husbandry and Veterinary Medicine of Fujian Academy of Agricultural Sciences confirmed for the first time that the pathogen of duck hemorrhagic necrotizing hepatitis is different from Muscovy duck reovirus and fowl reovirus from the aspects of virus physicochemical biology, animal regression test, genome characteristics and molecular level. The virus of the virus-named the novel duck reovirus (Novel Duck Reovirus, NDRV), so far, there is no effective prevention and treatment method for the disease