Preparation method for goose-origin reovirus inactivated vaccine

A technology of reovirus and inactivated vaccines, which is applied in the field of preparation of goose reovirus inactivated vaccines, can solve the problem that there is no better vaccine for goose reovirus, and achieve high protective effect

Inactive Publication Date: 2017-03-29
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

Currently, there is no good vaccine against goose reovirus

Method used

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  • Preparation method for goose-origin reovirus inactivated vaccine

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Embodiment

[0039] Reagent used in the present invention and component thereof are as follows:

[0040] Coating buffer: 1× carbonate buffer (100mL, pH=9.6): Na 2 CO 3 0.2756g, NaHCO 3 0.6216g, dissolve with distilled water and dilute to 100mL, pH value 9.5-9.7, store at 4°C;

[0041] PBS solution: NaCl 4.25g, NaH 2 PO 4 2H 2 O 0.178g, Na 2 HPO 4 12H 2 O 1.386g, dissolved in distilled water and adjusted to 500mL, pH value 7.1-7.3;

[0042] PBST diluent: add 0.5mL of Tween-20 per 1L of PBS, mix well;

[0043] PBST blocking solution: Dissolve 5g drymilk in 100mL PBST diluent, store at 4°C for short term and -20°C for long term.

[0044] TMB chromogenic solution:

[0045] Buffer A: Weigh 66.5063g of potassium citrate, dissolve it in 800mL of four-distilled water and adjust the pH value to 4.0 with concentrated hydrochloric acid, add 314μL of H 2 o 2 , and dilute to 1L with water. Store at 4°C;

[0046] Buffer B: Weigh 0.2956g of tetramethylbenzidine (TMB), dissolve 0.0633g of ...

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Abstract

The invention provides a preparation method for a goose-origin reovirus inactivated vaccine. The preparation method comprises the following steps that a goose embryo is inoculated against reoviruses through an allantoic cavity, dead embryo allantoic fluids or live embryo allantoic fluids are collected within 24-144 h after incubation, and reovirus fluids are obtained; the reovirus fluids are inactivated through formaldehyde, Tween-80 is added to be mixed to serve as a water phase, and white oil, Span-80 and aluminium stearate are mixed to serve as an oil phase; and the water phase is added into the oil phase to be mixed uniformly, and the inactivated vaccine is obtained. According to the preparation method, the prepared goose-origin reovirus inactivated vaccine has high protective effects on a gosling, and is suitable for virus immunity of the gosling.

Description

technical field [0001] The invention belongs to the technical field of veterinary vaccines, and in particular relates to a preparation method of an inactivated vaccine of goose-derived reovirus. Background technique [0002] Goose reovirus infection is a goose viral infectious disease caused by goose reovirus. [0003] The Reovirdae family includes Orthoreovirus, Rotavirus, Orbivirus, Cypovirus, Plant Reovirus Genus (Phytoreovirus) and Fijivirus (Fijivirus). Orthoreoviruses are a large group of double-stranded RNA viruses with envelopes. Current research shows that goose reoviruses that cause goose infection are Reoviridae and Orthoreoviruses subgroup II. [0004] The prevalence of goose reovirus infection has no obvious seasonality. It occurs in goslings and goslings aged 1 to 10 weeks, and mostly occurs in goslings of 2 to 4 weeks. Morbidity and mortality are closely related to age, with large differences. The incidence rate is 10% to 70%. The younger the age, the high...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/15A61P31/14
CPCA61K39/12A61K2039/5252A61K2039/552C12N2720/12234
Inventor 刁有祥张秉乾唐熠陈浩窦砚国
Owner SHANDONG AGRICULTURAL UNIVERSITY
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