Anti-tumor biological polysaccharide

A biological polysaccharide, anti-tumor technology, applied in the direction of anti-tumor drugs, drug combinations, etc., to improve the quality of life, high efficiency, and environmental protection.

Inactive Publication Date: 2013-06-12
乔民 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The technical problem to be solved in the present invention is to overcome the deficiencies of the existing extraction technology, aiming at the cumbersome steps of the original extraction technology - freeze-drying and organic solvent precipitation, the means of separation and purification of chicken embryo allantoic fluid polysaccharide and chicken embryo polysaccharide An innovation that uses a combination of salting out and membrane technology to extract biological polysaccharides

Method used

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Effect test

Embodiment 1

[0038] The preparation of embodiment 1 antitumor biological polysaccharide

[0039] The steps of the technique for extracting the polysaccharide in the present invention are as follows:

[0040] 1. Cultivate SPF chicken embryos at a constant temperature of 36-38°C for 9-13 days.

[0041] 2. Light inspection of the harvested chicken embryos, mark the position of the allantoic cavity, place it for 8 hours at a temperature of 4°C, and then extract live chicken embryos (the color of dead chicken embryos turns black, and the color of live chicken embryos turns red) ) allantoic fluid or chicken embryo culture.

[0042] 3. Extract the allantoic fluid or chicken embryo culture obtained in the previous step using the following purification process:

[0043] 1) Clarification of allantoic fluid or chicken embryo culture: use 10mM phosphate buffer to dilute allantoic fluid or chicken embryo culture and mix evenly, wherein the volume ratio of phosphate buffer to allantoic fluid or chicke...

Embodiment 2

[0049] The preparation of embodiment 2 antitumor biological polysaccharides

[0050] The steps of the technique for extracting the polysaccharide in the present invention are as follows:

[0051] 1. Cultivate SPF chicken embryos at a constant temperature of 36-38°C for 9-13 days.

[0052] 2. Light inspection of the harvested chicken embryos, marking the position of the allantoic cavity, placing them in a refrigerator at 4°C for 12-16 hours, and then extracting live chicken embryos (the color of dead chicken embryos turns black, and the color of live chicken embryos turns red ) allantoic fluid or chicken embryo culture.

[0053] 3. Extract the allantoic fluid or chicken embryo culture obtained in the previous step using the following purification process:

[0054] 1) Clarification of allantoic fluid or chicken embryo culture: Dilute allantoic fluid or chicken embryo culture 5 times with 20mM phosphate buffer, mix well, centrifuge at 12000g, 4°C for 20 minutes, collect superna...

Embodiment 3

[0060] The preparation of embodiment 3 antitumor biological polysaccharides

[0061] The steps of the technique for extracting the polysaccharide in the present invention are as follows:

[0062] 1. Cultivate SPF chicken embryos at a constant temperature of 36-38°C for 9-13 days.

[0063] 2. Light inspection of the harvested chicken embryos, mark the position of the allantoic cavity, place it for 48 hours at a temperature of 4°C, and then extract live chicken embryos (dead chicken embryos turn black, live chicken embryos turn red) ) allantoic fluid or chicken embryo culture.

[0064] 3. Extract the allantoic fluid or chicken embryo culture obtained in the previous step using the following purification process:

[0065] 1) Clarification of allantoic fluid or chicken embryo culture: use 100mM phosphate buffer to dilute allantoic fluid or chicken embryo culture and mix evenly, wherein the volume ratio of phosphate buffer to allantoic fluid or chicken embryo culture is 10:1, 12...

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Abstract

The invention discloses an anti-tumor biological polysaccharide; the polysaccharide is obtained from chick embryo allantoic fluid and chick embryo through separating and purifying; the invention further discloses a specific purification technology of the polysaccharide, wherein the technology is to extract the biological polysaccharide by a method combined with salting-out and membrane technology. By adopting the technology disclosed by the invention, the purifying purity and the recovering rate of chick embryo allantoic fluid and chick embryo are greatly enhanced; through preclinical pharmacodynamic experiments, the biological polysaccharide has an exact killing ability to transplant human body tumors such as human primary liver cancer and ovarian cancer in nude mice, and can completely remove the transplant human body tumor cells from the nude mice after being fed with enough dosage and enough time. The polysaccharide disclosed by the invention is a biological active material with no toxicity and low side effect and is very safe; in addition, the polysaccharide is wide in drug administration route, simple in production process and high in recovering rate, and thus being a novel anti-tumor drug capable of providing great social benefit and economic benefit.

Description

technical field [0001] The invention relates to the field of biological extraction. The extracted substance is a polysaccharide component derived from SPF chicken embryos. The specific method includes the whole process of SPF chicken embryo culture, harvest, separation and extraction of polysaccharide substances, and the analysis and detection of the polysaccharide substances. and uses. Background of the invention [0002] Tumor is a new organism formed by the body under the action of various carcinogenic factors, a certain cell in the local tissue loses its normal regulation of its growth at the gene level, resulting in abnormal clonal proliferation. In 2010, more than 8 million people will die of cancer in the world, replacing cardiovascular disease as the disease with the largest number of deaths in the world. Therefore, the research on cancer treatment drugs is related to the vital interests of liver cancer patients and the whole society, and has always been a research ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P35/00A61P35/02
Inventor 乔民金莲英
Owner 乔民
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