Multiple RT-PCR quick detection kit for five porcine enteroviruses and application thereof

A RT-PCR, detection kit technology, applied in recombinant DNA technology, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problems of long time, difficulty, low accuracy of serological methods, etc.
CN108715906AInactive Publication Date: 2018-10-30GUANGXI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
GUANGXI UNIV
Publication Date
2018-10-30
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a multiple RT-PCR quick detection primer group for five porcine enteroviruses. The primer group comprises five pairs of PCR primers which respectively aim at the five porcine enteroviruses PEDV, TGEV, PDCoV, PRoV and PKV and respectively have base sequences shown in a sequence table from SEQ. ID. NO.1 to SEQ. ID. NO.10. According to this, the inventor further develops a corresponding quick detection kit. The primer group has the advantages of high specificity, high sensitivity, short time consumption and low cost when being applied in a same reaction tube to detect single or mixed infection of the five porcine enteroviruses at the same time. A quick, simple and convenient tool is provided for detection of swinery mixed infection, a solid foundation is laid for clinical quick identification and detection and lab epidemiological investigation, timely formulating of treatment plans in porcine industry is facilitated, and swinery death rate and economic loss are reduced.
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Description

technical field

[0001] The invention belongs to the technical field of porcine enterovirus nucleic acid detection, in particular to a multiple RT-PCR rapid detection kit for five porcine enteroviruses and an application thereof. Background technique

[0002] In recent years, with the intensive and large-scale development of pig farming, the mixed infection of multiple pathogens in pig herds has become more and more common. Porcine epidemic diarrhea virus (PEDV), porcine transmissible gastroenteritis virus (TGEV), porcine Deltacoronavirus (PDCoV) and porcine rotavirus (PRoV) are the main pathogens of viral enteric disease in suckling piglets due to their pathological The similarities in clinical symptoms and epidemiology of the changes make it difficult to distinguish these diseases. At the same time, according to the inventor's monitoring of porcine ridge virus (PKV), it is found that its detection rate in PEDV-positive pigs is extremely high, and it is speculated that it i...

Claims

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