Heat stability improvement and efficient expression of phytase with high specific activity

A high-efficiency expression and thermal stability technology, applied in the field of improved phytase and its encoding gene, can solve the problems of poor thermal stability and large loss
CN1706941AInactive Publication Date: 2005-12-14安徽固源生物工程有限责任公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
安徽固源生物工程有限责任公司
Publication Date
2005-12-14
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention provides one kind of improved high specific activity phytase and its coding gene, and the improved phytase has even higher heat stability. The present invention also provides recombinant yeast cell containing the phytase gene of the present invention. The recombinant yeast of the present invention has phytase expressing amount up to 6.5E6 U in each ml of fermented liquid. The phytase of the present invention may be used widely in feed and food industry.
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Description

technical field

[0001] The invention relates to an improved phytase and its coding gene. The invention also relates to a recombinant yeast cell containing the phytase gene and capable of highly expressing the phytase. Background technique

[0002] Phytase (EC.3.1.3.8) is an enzyme that can hydrolyze phytic acid. It degrades phytate phosphorus into inositol and phosphoric acid. Phytase widely exists in microorganisms, such as Aspergillus in fungi (Yamada K. et al. Agric. Biol. Chem. 32: 1275-1282, 1986; Van Gorcom R.F.M. et al., US Patent No. 5436156, 1995) , Yeast (Nayini N.R.et al.Lebensm Wiss.Technol.17:24-26,1984); Bacillus subtilis (Paver, V.K.J., Bacteriol.151:1102-1108,1982) in bacteria, Pseudomonas (Cosgrove D.J., Austral.J.Biol.Sci.23:1207-1220, 1970), Lactobacillus (Shirai K., Letters Appl.Biol.Sci.19:366-369, 1994), Escherichia coli (Greiner R., Arch Biochem. Biophys. 303:107-113, 1993) et al.

[0003] Phytase can be widely used in monogastric animal feed (War...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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