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Lactobacillus separation strains with anti-inflammatory activity and application thereof

An isolate, the technology of the genus Lactobacillus, applied in the field of isolates of Lactobacillus with anti-inflammatory activity and its application, can solve the problem of reduced resistance to infection

Active Publication Date: 2009-11-11
GENMONT BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] 4. Corticosteroids: These drugs are very effective in treating rheumatoid arthritis, but they can have many side effects
Although the drug has the effect of reducing pain, stiffness and inflammation, long-term use may cause insomnia, tinnitus, edema, gastric ulcer, gastric bleeding, gastric perforation (gastrobrosia), skin sensitivity, proteinuria, diabetes, high blood pressure, bone Side effects such as osteoporosis, cataract, and decreased resistance to infection. At the same time, regular blood biochemical and serum examinations are required during the treatment to ensure the effectiveness of the treatment and avoid the occurrence of the above-mentioned side effects
[0017] To date, there is no drug that can effectively treat or relieve rheumatoid arthritis without producing unwanted side effects

Method used

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  • Lactobacillus separation strains with anti-inflammatory activity and application thereof
  • Lactobacillus separation strains with anti-inflammatory activity and application thereof
  • Lactobacillus separation strains with anti-inflammatory activity and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Example 1. Preliminary screening of Lactobacillus isolates with anti-inflammatory activity

[0072] Experimental materials and methods:

[0073] A. Source and preparation of test strains:

[0074] The applicant obtained the gastrointestinal tract specimens of many healthy adults through the Affiliated Hospital of China Medical University (Taichung City) in February 1991, and isolated more than 400 strains of Lactobacillus isolates from these specimens. In order to find probiotics with the potential to be used in the treatment of rheumatoid arthritis, applicants analyzed the anti-inflammatory activity of these Lactobacillus isolates and used IL-10 as a screening marker.

[0075] Each test strain was inoculated into Lactobacillus Bacto MRS broth medium (DIFCO, Cat. No. 0881), and then anaerobically cultured at 37° C. for 12 to 18 hours. The resulting bacterial culture was centrifuged at 4,000 rpm for 15 minutes, the supernatant was discarded, and the precipitate was was...

Embodiment 2

[0092] Example 2. Evaluation of Lactobacillus isolates stimulating the ability of human PBMC to secrete IL-10 and IFN-γ

[0093] Experimental materials and methods:

[0094] A. Preparation of human peripheral blood mononuclear cells:

[0095] Take human white blood cell concentrate (Tainan Blood Donation Center) and Ficoll-Paque that have passed the inspection and are used for experiments TM PLUS (17-1441-03, Amersham Biosciences) was used for density gradient centrifugation (720 g x 20 min at 4° C.) at a volume ratio of 1:1. Afterwards, the erythrocytes were removed, and the resulting human PBMCs were adjusted to a cell concentration of 4×10 in RPMI1640 medium containing 10% FBS. 6 cells / mL for later use.

[0096] B. Evaluation of Lactobacillus isolates stimulating human PBMC to secrete IL-10:

[0097] The ability of Lactobacillus isolates GMNL-19, GMNL-76, GMNL-78, GMNL-89, GMNL-94, GMNL-101 and GMNL-161 to stimulate human PBMC to secrete IL-10 is basically the "experim...

Embodiment 3

[0119] Example 3. Evaluation of the therapeutic effect of Lactobacillus isolates GMNL-76 and GMNL-89 on rats with collagen-induced arthritis

[0120] Experimental materials and methods:

[0121] A. Experimental animals:

[0122] Male LEW / SsNNarl rats (6 weeks old, weighing approximately 200 to 250 g) obtained from the National Applied Research Laboratories National Laboratory Animal Center were used in the following experiments. All experimental animals were kept in an individually air-conditioned animal room with 12 hours of light and 12 hours of darkness, room temperature maintained at 25±1°C and humidity maintained at 60±5%, and water and feed were adequately supplied. Give the animals a week to acclimate to the environment, and start the experiment after the animals are stable. The handling of experimental animals and all experimental procedures are carried out in accordance with the standard regulations of the Experimental Animal Committee.

[0123] B. Preparation of t...

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PUM

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Abstract

The invention discloses two lactobacillus separation strains with anti-inflammatory activity and beneficial properties, which are Lactobacillus sakei GMNL-76 and Lactobacillus reuteri GMNL-89. The two lactobacillus separation strains are respectively preserved in the Bioresouce Collection and Research Center (BCRC) of the Food Industry Research and Development Institute (FIRDI) by preservation numbers of BCRC 910355 and BCRC 910340 and preserved in the China Center for Type Culture Collection (CCTCC) by the preservation numbers of CCTCC M 207153 and CCTCC M 207154. The two lactobacillus separation strains and subculture offspring thereof can be used for preparing various food products and pharmaceutical composition for curing and / or mitigating diseases (such as rheumatoid arthritis) relevant to inflammation.

Description

technical field [0001] The present invention relates to two Lactobacillus isolates having anti-inflammatory activity and beneficial probiotic properties, namely Lactobacillus sakei GMNL-76 and Lactobacillus reuteri GMNL-89, which were deposited in the Biological Resource Conservation and Research Center (BCRC) of the Food Industry Development Institute (FIRDI) under the deposit numbers BCRC910355 and BCRC 910340, and were deposited in the Chinese Typical Culture Collection Center (CCTCC). These two strains of Lactobacillus isolates and their subcultured progeny can be used to prepare various food products, as well as for the preparation of medicines for the treatment and / or alleviation of diseases associated with inflammation, such as rheumatoid arthritis. pharmaceutical composition. Background technique [0002] Rheumatoid arthritis (RA) is a chronic autoimmune disease that causes swelling, deformation, pain, atrophy, and stiffness in joints (such as hands, feet, wrists, ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/74A61K9/00A61P19/02A61P29/00A23L1/30C12R1/225A23L33/135A61K35/747
Inventor 俞笃文李浚笙谢丰钦陈靓珮黄璻尹吕英震
Owner GENMONT BIOTECH
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