Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Protein-polyphenol covalent compound and preparation method and application thereof

A protein and complex technology, applied in the field of food science and food additives, can solve the problems of poor physical and chemical stability, and achieve the effects of good safety, simple preparation method, high physical and chemical stability and bioavailability

Inactive Publication Date: 2016-05-25
CHINA AGRI UNIV
View PDF2 Cites 43 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the β-carotene emulsion prepared with a single protein as an emulsifier has good safety, it is easily affected by temperature, pH value, ionic strength, etc., and its physical and chemical stability is poor.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Protein-polyphenol covalent compound and preparation method and application thereof
  • Protein-polyphenol covalent compound and preparation method and application thereof
  • Protein-polyphenol covalent compound and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] First use 0.5mol / L sodium hydroxide solution to adjust the pH value of deionized water to 9; then weigh 0.5g α-lactalbumin, β-lactoglobulin, lactoferrin and sodium caseinate, and dissolve them in 50mL pH. In deionized water with a value of 9, four milk protein solutions are formed; 0.1g of epigallocatechin gallate (EGCG) is dissolved in 50 mL of deionized water with a pH of 9 to form an EGCG solution;

[0034] Under the condition of constant stirring at 120 rpm, the EGCG solution was added to the four milk protein solutions and air was introduced to form a reaction system; then the reaction system was stirred at a constant speed of 120 rpm at room temperature for 24 hours for reaction; The sample was dialyzed at 4°C and 60KHz ultrasonic frequency for 48h, and the water was changed every 3h to remove unreacted free EGCG, and then the remaining liquid in the dialysis bag was freeze-dried to obtain four milk protein-EGCG covalent complexes Things.

[0035] Differential scannin...

Embodiment 2

[0040] Take the four milk proteins and the four milk protein-EGCG covalent complexes in Example 1, and use 10mM sodium phosphate buffer (pH=7.0) to prepare 0.02-0.3mg / mL protein solution or protein-poly Phenol covalent complex solution, while preparing 8mM 8-anilino-1-naphthalenesulfonic acid (ANS) solution. Add 20 μL of ANS solution to 4 mL of the above protein solution and mix well. Excite the fluorescence spectrum at a wavelength of 390nm, measure the fluorescence intensity at a wavelength of 470nm, and plot the relative fluorescence intensity of the sample against the protein concentration. The linear slope of the initial segment is the surface hydrophobicity index of the sample. The measurement results are shown in Table 2. Show.

[0041] Table 2: Comparison of the surface hydrophobicity index of milk protein and milk protein-EGCG covalent complex

[0042]

[0043] It can be seen from Table 2 that the surface hydrophobicity index of milk protein is significantly reduced afte...

Embodiment 3

[0045] Take the four milk proteins and the four milk protein-EGCG covalent complexes in Example 1, and use 10mM sodium phosphate buffer (pH=7.0) to prepare 0.02-0.3mg / mL protein solutions; The solution was added to 3 mL of ABTS solution, and after reacting for 60 minutes at room temperature in the dark, the absorbance was measured at a wavelength of 734 nm. The ability of the sample to scavenge ABTS free radicals is compared with that of water-soluble vitamin E (Trolox). The ability of the sample to scavenge ABTS free radicals is expressed by Trolox equivalent (nmolTE / g dry basis). The measurement results are shown in Table 3.

[0046] Table 3: Comparison of ABTS free radical scavenging ability of milk protein and milk protein-EGCG covalent complex

[0047]

[0048] Note: The unit of ABTS free radical scavenging ability in the above table is nmolTE / g dry basis.

[0049] It can be seen from Table 3 that the ABTS free radical scavenging ability of the milk protein-EGCG covalent comple...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of food science and food additives and discloses a protein-polyphenol covalent compound and a preparation method and application thereof. The preparation method includes that the protein-polyphenol covalent compound is prepared by free radical grafting copolymerization or base-induced reaction of protein and polyphenol. The preparation method is simple and avoids toxic chemical reagents and high-temperature high-pressure conditions, all processes are carried out in aqueous phase, and the requirement on environment friendliness is met. Beta-carotene emulsion prepared by taking the protein-polyphenol covalent compound as an emulsifying agent is high in safety, physical and chemical stability and bioavailability and is widely applicable to the technical fields of food industry, biomedicine, hygiene and the like.

Description

Technical field [0001] The invention belongs to the technical field of food science and food additives, and specifically relates to a protein-polyphenol covalent complex and a preparation method and application thereof. Background technique [0002] As a kind of carotenoid, β-carotene has strong antioxidant activity, which can effectively reduce the incidence of cardiovascular disease and cancer and improve human immunity. However, β-carotene is a fat-soluble compound, which is difficult to apply to aqueous systems; it is sensitive to light, heat, and oxygen, and is prone to degradation; it is less absorbed in the body and has low bioavailability, which obviously limits the use of β-carotene in food. Widely used in fields such as medicine and medicine. The preparation of β-carotene into an emulsion can not only solve the solubility problem of β-carotene, but also improve its stability, and at the same time has the advantages of targeting and slow release. [0003] Small molecule ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/107A61K31/015A23D9/04
CPCC07K1/1077A23D9/04A61K31/015C07K19/00
Inventor 高彦祥刘夫国魏子淏
Owner CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com