Aeromonas salmonicida vaccine and application thereof
A technology of Aeromonas salmonicida and vaccine, which is applied in the field of microbiology and immunology of aquaculture animals, can solve the problems of no specific vaccine and serious disease of half-smooth tongue sole, achieve good immune protection, increase the drug resistance of pathogenic bacteria, reduce loss effect
Inactive Publication Date: 2018-12-07
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Problems solved by technology
[0005] In order to solve the problem that tongue sole is seriously diseased and there is no specific vaccine, we proposed the Aeromonas salmonicida vaccine and its application
Method used
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Embodiment 1
[0023] Isolation of CS-1 strain from sick tongue sole
[0024] The diseased half-smooth tongue sole was collected from a farm in Weifang, Shandong Province, with a body length of about 20 cm. It was cultured in an industrialized manner with a water temperature of about 20 °C. Sampling was taken from the liver, spleen, and kidney of the diseased tongue sole, streaked on the TSA plate medium, cultured in a 28°C incubator for 48 hours, and the obtained dominant colonies were purified and cultured, and the purified cultured bacteria were stored in a -80°C refrigerator with glycerol spare.
[0025] Drug Sensitivity Analysis of CS-1 Strain
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An Aeromonas salmonicida subspecies (Aeromonas almonicidasubsp. masoucida) is isolated from Cynoglossus semilaevis Gunther cultured in Shandong Province, China, the preservation number of the Aeromonas almonicidasubsp. masoucida is CCTCCM2018085. A pathogenic bacteria strain of ascites disease is isolated from the Cynoglossus semilaevis Gunther for the first time. Antigens of the strain contain protective antigens against Aeromonas salmonicida, the strain is suitable for preparing various vaccines against the Aeromonas salmonicida, and is capable of reducinge losses caused by diseases in aquaculture, reducing or eliminating abuse of chemical drugs such as disinfectants and antibiotics caused by the Aeromonas salmonicida, and significantly improving the safety of aquaculture animals and products, and reducing bio-safety risks caused by increase of drug resistance of pathogenic bacteria.
Description
technical field [0001] The invention relates to the field of microbiology and immunology of aquaculture animals, in particular to an Aeromonas salmonicida vaccine and its application. Background technique [0002] Cynoglossus semilaevls (Cynoglossus semilaevls) belongs to Pleuronectiforme, Cynoglossidae, and Cynoglossus. There are many in the Yellow Sea, which is a traditional rare fish in our country. In recent years, with the continuous increase of artificial breeding scale and breeding density, tongue sole diseases occur frequently, which seriously affects the development of tongue sole industry. According to the existing literature reports, Listonella anguillarum (Zhang Xiaojun et al., 2009), Vibrio parahaemolyticus (Liang Liguo et al., 2010), Vibrio aestuarianu (Zheng Faxin et al., 2009) , Photobacterium damselae subsp.piscicida (Wang et al, 2007), Edwardsiella tarda (Bai Fangfang et al, 2009) and Aeromonas salmonicida (Zhou Hongxia et al, 2017) and other pathogenic ...
Claims
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Login to View More IPC IPC(8): A61K39/104A61P31/04
CPCA61K39/104A61K2039/552A61P31/04
Inventor 李杰莫照兰刘长琳李贵阳陈四清
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI