Pathogenic aeromonas hydrophila assay kit

Abstract

The invention relates to a pathogenic aeromonas hydrophila assay kit and belongs to the field of the detection of pathogenic microorganisms. A primer which is designed on the basis of a highly conserved interval of a standard strain J-1 strain 16S rDNA genome of the pathogenic aeromonas hydrophila is used for distinguishing and identifying strain characteristics of the pathogenic aeromonas hydrophila, and the amplified fragment is 685bp; and a second pair of primers which is designed according to specific proteinase aerolysin secreted by the strain is used for judging the pathogenicity of the aeromonas, and the fragment size is 301bp. The assay kit can rapidly and accurately judge the strain characteristics and pathogenicity of the strains to be tested. Comparison tests of the conventional biochemical identification method and pathogenicity identification method for experiment strains and clinical stains show that the coincidence rate of the kit is 100 percent. Evaluation tests for specificity, repeatability, sensitivity and retention period of the kit acquire ideal results.

Description

technical field [0001] The invention relates to a pathogenic Aeromonas hydrophila detection kit, which belongs to the field of pathogenic microorganism detection and relates to the detection of pathogenic Aeromonas hydrophila for humans and susceptible animals. Background technique [0002] Aeromonas has a wide range of pathogenicity. It infects a variety of animals including cold-blooded animals such as fish, poultry and mammals, causing local infections such as sepsis or skin ulcers. It is the most common pathogenic bacteria in aquatic animals, especially fish. Germs, Aeromonas mobilis can cause outbreaks of fish bacterial sepsis in summer when the water temperature is high. Human infection with Aeromonas mobilis causes acute gastroenteritis, etc. At present, this bacteria has been included in the routine detection range of diarrhea pathogens in foreign countries, and it is the object of food hygiene inspection. Aeromonas hydrophila has also been found in human cases of s...

AI Technical Summary

Problems solved by technology

Although 5S rRNA is easy to analyze, due to the lack of nucleotides, there is not enough genetic information for taxonomic research

The number of nucleotides contained in 23S rRNA is almost twice that of 16S rRNA, so it is more difficult to analyze

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