Analysis method and system for identifying heat treatment degree and doping of liquid milk

An analysis method and technology for liquid milk, applied in the direction of analyzing materials, material analysis by electromagnetic means, character and pattern recognition, etc., can solve the problems of sterilization conditions cannot be verified, and cold chain technology cannot be tracked in the whole process, reaching Qualcomm The effect of high volume, simple operation and accurate identification

Pending Publication Date: 2022-02-01
SHANDONG ANALYSIS & TEST CENT
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, taking imported pasteurized milk as an example, the shelf life of the product is much higher than the longest shelf life of pasteurized fresh milk, and because the place of production is not in China, the pasteurization method and sterilization conditions used cannot be verified, and cold chain technology cannot be carried out. Tracking the whole process, consumers often fall into the "consumption trap" when buying imported pasteurized milk

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Analysis method and system for identifying heat treatment degree and doping of liquid milk
  • Analysis method and system for identifying heat treatment degree and doping of liquid milk
  • Analysis method and system for identifying heat treatment degree and doping of liquid milk

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] 1. Select enough commercially available pasteurized milk, extended shelf-life pasteurized milk, and ultra-high temperature sterilized milk, and measure the corresponding volume of milk respectively, and take 30ml for each type of milk;

[0058] 2. Centrifuge each milk sample at 4°C and 1100×g (2×1h), remove the upper fat layer, and perform the degreasing process;

[0059] 3. Take 1ml of skim milk samples with different heating degrees, each sample is paralleled 20 times, add 3ml of acetonitrile solution respectively, carry out organic solvent precipitation protein, let it stand for half an hour, and take the supernatant;

[0060] 4. Mix 5 μl supernatant sample with 5 μl saturated CHCA matrix solution for each parallel, and place it for testing;

[0061] 5. Take 1 μl of the mixed sample to be tested, spot the sample on the target plate, and let it dry naturally. After drying and crystallization, put it into the MALDI-TOF-MS instrument for scanning and detection, and obta...

Embodiment 2

[0071] 1. Select enough commercially available pasteurized milk and ultra-high temperature sterilized milk, according to the volume ratio (pasteurized milk: ultra-high temperature sterilized milk) 1:1, 2:1, 3:1, 4:1, 5:1 , respectively measure the corresponding volume of milk for mixing, the total volume is 30ml, vortex for 1 minute, and mix well;

[0072] 2. Centrifuge the mixed milk sample at 4°C and 1100×g (2×1h), remove the upper fat layer, and perform the degreasing process;

[0073] 3. Take 1ml of skim milk samples with different volume ratios, each sample is paralleled 20 times, add 3ml of acetonitrile solution respectively, carry out organic solvent precipitation protein, let it stand for half an hour, and take the supernatant;

[0074] 4. Mix 5 μl supernatant sample with 5 μl saturated CHCA matrix solution for each parallel, and place it for testing;

[0075] 5. Take 1 μl of the mixed sample to be tested, spot the sample on the target plate, and let it dry naturally....

Embodiment 3

[0085] 1. Select enough commercially available extended-shelf-life pasteurized milk and UHT milk, according to the volume ratio (extended shelf-life pasteurized milk: UHT milk) 1:1, 2:1, 3:1, 4 :1, 5:1, measure the corresponding volume of milk for mixing, the total volume is 30ml, vortex for 1 minute, and mix well;

[0086] 2. Centrifuge the mixed milk sample at 4°C and 1100×g (2×1h), remove the upper fat layer, and perform the degreasing process;

[0087] 3. Take 1ml of skim milk samples with different volume ratios, each sample is paralleled 20 times, add 3ml of pure acetonitrile solution respectively, carry out organic solvent precipitation protein, let it stand for half an hour, and take the supernatant;

[0088] 4. Take 5ul supernatant samples in parallel and mix them with 5μl saturated CHCA matrix solution, and place them for testing. After drying the crystals, put them into a MALDI-TOF-MS instrument for scanning detection to obtain the corresponding mass spectrum. The...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the technical field of food safety detection, in particular to an analysis method and system for identifying the heat treatment degree and doping of liquid milk, and the method comprises three steps of sample pretreatment, mass spectrometry and data analysis. According to the invention, a machine learning technology and mass spectrometric detection are combined to establish a milk identification method and system with different heating degrees. In this way, a model is established by utilizing a machine learning algorithm according to the difference of polypeptide composition and content change of milk of different heated types in mass spectrum information, and information of a heat-sensitive peptide fragment is obtained. Then the model is continuously trained and optimized, a powerful prediction model is screened out, and therefore efficient, stable and accurate identification is achieved. The identification method and system are scientific and effective. The method established by the invention has the advantages of simplicity and convenience in operation, low organic solvent consumption, high throughput, high prediction accuracy and the like.

Description

technical field [0001] The invention relates to the technical field of food safety detection, in particular to an analysis method and system for identifying the heat treatment degree and doping of liquid milk by combining machine learning and mass spectrometry technology. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Liquid milk is a natural food rich in nutrients, suitable in proportion, easy to digest and absorb, and high in nutritional value. In particular, milk is known as white blood and is called "the closest to perfect food" for human beings. Milk contains a variety of nutrients, mainly including protein, vitamins, minerals, growth factors and other nutrients, wh...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/626G06K9/62
CPCG01N27/628G06F18/241
Inventor 陈相峰张世山赵燕芳李慧娟丁宁
Owner SHANDONG ANALYSIS & TEST CENT
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap