Method for constructing liquid chromatography fingerprint spectrum of abrus cantoniensis hance amide components

Abstract

The invention belongs to the technical field of medicine quality control, and particularly relates to a construction method of a liquid chromatography fingerprint spectrum of an abrus cantoniensis hance amide component. The fingerprint spectrum of the abrus cantoniensis hance amide component is obtained through a high performance liquid chromatograph. The method has the characteristics of being stable in identification, good in precision and reproducibility, high in separation degree, easy to operate and the like. In the fingerprint spectrum of the abrus cantoniensis hance amide component, thepeaks are uniformly distributed, and the common peak characteristic advantage is more obvious. The quality condition of the abrus cantoniensis hance amide component can be monitored from the overallappearance characteristic of chromatography. The method can be applied to identification, quality analysis and evaluation of the abrus cantoniensis hance amide component and can also be applied to identification, quality evaluation and control of abrus cantoniensis hance medicinal materials and related preparations and products containing amide extracts.

Description

technical field [0001] The invention belongs to the technical field of drug quality control, and in particular relates to a method for constructing a liquid chromatography fingerprint of chicken bone oxamide components. Background technique [0002] With the realization of the modernization of traditional Chinese medicine, it has gradually become a consensus that a complete quality control method for traditional Chinese medicine should be established, and many countries have accepted the quality control method of chromatographic fingerprints. Fingerprint of traditional Chinese medicine is a spectrum that characterizes and describes the chemical information of a certain chemical substance or each chemical substance in the chemical composition of traditional Chinese medicine in the form of graphics (images) from both qualitative and quantitative perspectives by means of modern instrumental analysis methods. It can more comprehensively reflect the relative relationship of the c...

AI Technical Summary

Problems solved by technology

Previous studies have found that Abrusamide is rich in amide components, such as Abrusamide A, Abrusamide B, Abrusamide B, and N-trans-p-coumaroyltyramide acid, etc., the amide component has good anti-oxidation, anti-inflammatory, liver protection and other activities, and has obtained patent protection (ZL201210421480.X), and established an extraction and preparation method for this part (invention patent application 201610839080.9), but its quality evaluation The method has not obtained good separation conditions, especially the components of this type are isomers and conformational isomerism, which has always been a difficulty in separation and analysis

[0003] Moreover, the quality control of Jigucao under the 2015 edition of the "Chinese Pharmacopoeia" is based on trait identification and thin-layer chromatography of reference medicinal materials. The methods of quality evaluation and quality identification are relatively monotonous, and only rely on trait identification and TLC It is difficult to better evaluate Jigucao by chromatographic analysis, especially for other types of components and effective parts except abrin. There is no identification and determination method for active parts, and it is difficult to determine the quality of Jigucao medicinal materials and their preparations. Provide technical support and basis for control and evaluation

[0004] Therefore, for amides components, in order to better carry out problems such as its quality evaluation and application, the present invention establishes a method for constructing liquid chromatography fingerprints of chicken bone oxalamides components. The method has high resolution, high It has the characteristics of high precision, strong operability, good stability and reproducibility, etc., and overcomes the defect of poor separation under common analysis conditions. Identification, quality evaluation and control of related preparation products

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