A method for constructing liquid chromatography fingerprints of chicken bone oxalamide components

A chicken bone oxamide and liquid chromatography technology, applied in the field of drug quality control, can solve the problems of providing technical support and basis, difficult separation and analysis, poor resolution, etc., and achieves obvious advantages of common peak characteristics, precision and weight Good reproducibility and uniform distribution of peaks

Active Publication Date: 2022-06-17
GUANGDONG PHARMA UNIV
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous studies have found that Abrusamide is rich in amide components, such as Abrusamide A, Abrusamide B, Abrusamide B, and N-trans-p-coumaroyltyramide acid, etc., the amide component has good anti-oxidation, anti-inflammatory, liver protection and other activities, and has obtained patent protection (ZL201210421480.X), and established an extraction and preparation method for this part (invention patent application 201610839080.9), but its quality evaluation The method has not obtained good separation conditions, especially the components of this type are isomers and conformational isomerism, which has always been a difficulty in separation and analysis
[0003] Moreover, the quality control of Jigucao under the 2015 edition of the "Chinese Pharmacopoeia" is based on trait identification and thin-layer chromatography of reference medicinal materials. The methods of quality evaluation and quality identification are relatively monotonous, and only rely on trait identification and TLC It is difficult to better evaluate Jigucao by chromatographic analysis, especially for other types of components and effective parts except abrin. There is no identification and determination method for active parts, and it is difficult to determine the quality of Jigucao medicinal materials and their preparations. Provide technical support and basis for control and evaluation
[0004] Therefore, for amides components, in order to better carry out problems such as its quality evaluation and application, the present invention establishes a method for constructing liquid chromatography fingerprints of chicken bone oxalamides components. The method has high resolution, high It has the characteristics of high precision, strong operability, good stability and reproducibility, etc., and overcomes the defect of poor separation under common analysis conditions. Identification, quality evaluation and control of related preparation products

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for constructing liquid chromatography fingerprints of chicken bone oxalamide components
  • A method for constructing liquid chromatography fingerprints of chicken bone oxalamide components
  • A method for constructing liquid chromatography fingerprints of chicken bone oxalamide components

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0030] (1) Instruments and drugs: use an Agilent 1290 ultra-high performance liquid chromatograph. Twenty-four batches of chicken bone herbs from different origins and harvest periods were identified as the dried leaves of the leguminous plant, Abrus cantoniensis Hance or Abrus mollis Hance. N-trans-p-coumaroyl tyrosine, chitosan A, chitosan B and chitosan C reference substances (self-made by the New Drug Research and Development Center of Guangdong Pharmaceutical University). Methanol and acetonitrile are of chromatographic grade, other reagents are of analytical grade, and water is pure water. The concentration percentages of the reagents in this test are all volume percentages.

[0031](2) Preparation of the test solution: Weigh 2 g of chicken bone herbal medicine, accurately weigh it, place it in a conical flask, add 60 mL of 50% ethanol solution, heat under reflux for 3 times, 1 h each time, filter, and combine the filtrates , concentrated under reduced pressure until t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the technical field of drug quality control, and in particular relates to a method for constructing a liquid chromatography fingerprint of chicken bone oxamide components. In the present invention, the fingerprints of the chicken bone oxamide components are obtained by means of a high-performance liquid chromatograph. The method has the characteristics of stable identification, good precision and reproducibility, high resolution, and easy operation. The obtained fingerprints of chicken bone oxamide components have even distribution of peaks, and the advantages of common peak characteristics are more obvious. It can be obtained from the chromatogram It can be used for the identification, quality analysis and evaluation of chicken bone oxamide components, as well as related preparations of chicken bone herbal medicines and amide-containing extracts. , Product identification, quality evaluation and control.

Description

technical field [0001] The invention belongs to the technical field of drug quality control, and in particular relates to a method for constructing a liquid chromatography fingerprint of chicken bone oxamide components. Background technique [0002] With the realization of the modernization of traditional Chinese medicine, it has gradually become a consensus that a complete quality control method of traditional Chinese medicine should be established, and many countries have accepted the quality control method of chromatographic fingerprint. The fingerprint of traditional Chinese medicine is a spectrum that characterizes and describes the chemical information of a certain type of chemical substance or each chemical substance in the chemical composition of traditional Chinese medicine in a graphical (image) manner from both qualitative and quantitative perspectives with the help of modern instrumental analysis methods. It can more comprehensively reflect the relative relations...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/86
CPCG01N30/88G01N30/8686G01N30/8679G01N2030/8809G01N2030/027
Inventor 袁旭江门丽娇刘亚迪邱雨李坤平
Owner GUANGDONG PHARMA UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products