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Beluga artificial propagation method and application of carp pituitary gland

A carp pituitary gland and artificial breeding technology, which is applied in the field of beluga breeding, can solve the problems such as the degradation of the artificial breeding germplasm of beluga, and achieve the effects of good fertilization and hatching effect, easy acquisition and preparation, and good survival status

Inactive Publication Date: 2016-11-09
BEIJING FISHERMAN AQUATIC PROD TECH DEV CENT +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the defects of the prior art such as the severe degradation of the germplasm of the artificial breeding of the Chinese beluga, and to provide a method for rapid artificial breeding of the beluga and the application of the carp pituitary gland

Method used

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  • Beluga artificial propagation method and application of carp pituitary gland
  • Beluga artificial propagation method and application of carp pituitary gland

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0053] This preparation example is used to illustrate the preparation of the carp pituitary gland solution of the drug used to induce the production of beluga beluga of the present invention.

[0054] Carp pituitary gland dry matter 200mg, weighed and then crushed and dissolved in 10ml 15% by weight glucose solution, 2.2ml 50% by weight glucose solution and 7.8ml 5% by weight glucose solution for 10ml 15% by weight glucose solution Prepared with glucose solution.

preparation example 2

[0056] This preparation example is used to illustrate the preparation of the carp pituitary gland solution of the drug used to induce the production of beluga beluga of the present invention.

[0057] Carp pituitary gland dry matter 228mg, after weighing, crush and dissolve in 12ml of 10% by weight glucose solution. 10ml of 15% by weight glucose solution needs to be prepared with 1.3ml of 50% by weight glucose solution and 13.7ml of 5% by weight glucose solution.

Embodiment 1

[0059] This example is used to illustrate the method of artificial reproduction of A. hulus and the application of oxytocic drugs in the artificial reproduction of A. hulus.

[0060] (1) 8 months before the need to induce labor, select the female parent of A. hulus (which has been cultivated for 13 years, and its egg diameter has reached 3.3mm, and its weight is 50kg) and the male parent of . semen, body weight 50kg), and then carry out nutrient-intensive cultivation on the female and male parents of the beluga beluga for 150 days. 3 , the water temperature is 20°C, so that the dissolved oxygen is 6mg / L, the ammonia nitrogen content is 0.4mg / L, and the nitrite is 0.05mg / L; the control feeding method is: feed once a day, and the feeding rate is 0.3%. The protein content in the compound feed is 83% by weight, the fat content is 9.9% by weight, the crude fiber content is 5% by weight, the lecithin is 2% by weight, and the vitamin E content is 3% by weight;

[0061] (2) Check the...

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Abstract

The invention relates to the field of beluga culture, particularly discloses a beluga artificial propagation method and application of a carp pituitary gland, and relates to a beluga artificial propagation method. The method comprises the steps that 1, nutrient enrichment culture is performed on a beluga milter parent and a beluga raun parent; 2, the beluga milter parent and the beluga raun parent obtained after nutrient enrichment culture are subjected to low temperature stimulation; 3, the water temperature is increased to the pre-spawning induction temperature, and spawning induction medicine administration is performed on the beluga milter parent and the beluga raun parent so as to be subjected to spawning induction; 4, free eggs and seminal fluid are collected to be subjected to artificial fertilization and incubation, wherein the spawning induction medicine is the carp pituitary gland. The invention further relates to application of the carp pituitary gland for beluga artificial propagation, and application of the carp pituitary gland for preparing medicine for artificial propagation of beluga. The beluga spawning induction medicine is easy to obtain and prepare, the spawning induction rate of spawning induction propagation performed by adopting the beluga spawning induction medicine is high, the fertilization incubation effect is good, and the postpartum parent fish survival condition is good.

Description

technical field [0001] The invention relates to the field of beluga breeding, in particular to a method for artificial breeding of beluga and the application of carp pituitary gland. Background technique [0002] Huso huso is a large fish, a ferocious carnivorous fish, with the advantages of good meat quality and fast growth. It is one of the largest freshwater fishes in existence, with a weight of more than 1 ton. Its sexual maturity takes a long time, and it takes more than 12 years under artificial breeding conditions. The caviar made from its eggs is the best in caviar, and the price is several times or more than ten times higher than other sturgeons. It was introduced to China for the first time in 2002, and its induced reproduction is different from ordinary sturgeons. In China, only Ren Hua et al. (article number: 1006-3188(2013)-01-0020-03) researched on the artificial reproduction technology of the sturgeon in 2013. , in this document, artificial injection of dioco...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00A61K35/60A61P15/08
CPCA01K61/00A61K35/60Y02A40/81
Inventor 齐茜刘晓勇依布拉英·买丽坎白喜军
Owner BEIJING FISHERMAN AQUATIC PROD TECH DEV CENT
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