The invention discloses a gynecological disease resisting gel for treating gynecological disease and a preparation method. The gynecological disease resisting gel comprises the following components in parts by weight: 225-275 parts of radix sophorae flavescentis, 225-275 parts of polygonum perfoliatum, 135-165 parts of amur corktree bark, 45-55 parts of fructus forsythiae, 27-33 parts of motherwort herb, 27-33 parts of red bean, 27-33 parts of folium artemisiae argyi, 27-33 parts of Chinese angelica, and 27-33 parts of combined spicebush root. The preparation method of the gynecological disease resisting gel is as follows: crushing 160 parts of the total amount of the radix sophorae flavescentis, 90 parts of the total amount of the amur corktree bark, 15 parts of the total amount of the fructus forsythiae and 15 parts of the total amount of the red bean into fine powder for later use; adding water into nine medicine materials such as the rest of radix sophorae flavescentis, polygonum perfoliatum and the like, decocting for two times with the first time for 2 hours and the second time for 1 hour; combining decocted liquid, filtering to obtain filtered liquid, concentrating the filtered liquid to thick paste with the relative density of 1.31-1.35 at the temperature of 60-80 DEG C, adding the fine powder, and mixing uniformly; taking a medium, adding water for swelling, and preparing into 0.1-0.5% glue solution; and adding 2.5-5 parts of medicinal thick paste into 2.5-5 parts of glue solution, adding water under stirring, preparing into 1-4 parts of 30% wetting agent solution, and stirring uniformly, thus obtaining the gynecological disease resisting gel. By utilizing the invention, the dosing frequency can be greatly reduced, the treatment cycle is short, and the efficiency is high.