Method for establishing animal model for senile dementia, special liquid medicine and dosing device

A technology of Alzheimer's disease and animal models, applied in the field of neuroscience, can solve problems such as injury, difficulty in widespread promotion, and inability to obtain data, and achieve the effects of easy operation, strong practicability, and injury avoidance

A technology of Alzheimer's disease and animal models, applied in the field of neuroscience, can solve problems such as injury, difficulty in widespread promotion, and inability to obtain data, and achieve the effects of easy operation, strong practicability, and injury avoidance

CN103284980AInactive Publication Date: 2013-09-11KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Method for establishing animal model for senile dementia, special liquid medicine and dosing device
  • Method for establishing animal model for senile dementia, special liquid medicine and dosing device
  • Method for establishing animal model for senile dementia, special liquid medicine and dosing device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Materials: Take 6 healthy young male rhesus monkeys aged 4-8, weighing 5kg-9kg. Four of them were used to inject formaldehyde into the lateral ventricle; two were used as a blank control (only given saline). Formaldehyde is produced by Tianjin Fuyu Fine Chemical Co., Ltd.

[0037] For the structure of the drug delivery device, see figure 1 . It includes a catheter 1, a drug delivery tube 2 that can move freely in the catheter 1, and a plugging column 3 that can be inserted into the upper port of the catheter 1 to block the entire lumen of the catheter 1. The inner diameter of the catheter 1 is 0.75 mm. The outer diameter is 1.0 mm, the length is 30 mm, the inner diameter of the drug delivery tube is 0.5 mm, and the length of the drug delivery tube exceeds the catheter length by 0.5 mm. The drug delivery device is made of stainless steel. figure 1 Middle 4 is the limiting head.

[0038] Steps: 1. Embedding tubes in the left and right ventricles of the experimental a...

Embodiment 2

[0041] The difference between this example and Example 1 is only that: the volume percentage concentration of formaldehyde saline solution is 5‰, each animal is injected with 200 microliters per day, and the AD non-human primate model can be obtained by continuous administration for 80 days, but Compared with Example 1, the modeling effect is slightly worse.

Embodiment 3

[0043] The only difference between this example and Example 1 is that the volume percentage concentration of methanol physiological saline solution is 3%, and each animal is injected with 100 microliters per day, and the AD non-human primate model can be obtained by continuous administration for 150 days.

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Abstract

The invention relates to a method for establishing an animal model for senile dementia, a special liquid medicine and a dosing device, and belongs to the technical field of neurosciences. The method comprises the following steps: obtaining positioning coordinates of paraceles according to brain atlases or/and magnetic resonance image data of an experimental animal; respectively burying a conduit of the dosing device in left and right paraceles of the animal, and injecting the special liquid medicine to the paraceles at a constant speed in 14-16 minutes under a sterile environment within 2-3 weeks, wherein the liquid medicine amount is 100-250 ml methanol per day; alternately dosing the left and right paraceles, wherein the dosing frequency is 18-30 hours at an interval; and continuously dosing for 2-10 months to obtain a non-human primate animal model for senile dementia. With the adoption of a novel dosing mode: dosing to the paraceles at the constant speed, medicine can circulate to whole brain through the cerebrospinal fluid to exert the toxic effect, so that peripheral damage by the medicine is avoided. The AD model is established by injecting methanol or formaldehyde to the paraceles, so that behaviors and pathological symptoms are typical, the basic physiological status is good and the individual difference is relatively smaller.

Description

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Claims

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Application Information

Patent Timeline
11 Sep 2013
Publication
CN103284980A