Method for artificial fertilization of sturgeon

Abstract

The invention discloses a method for artificial fertilization of sturgeon. The method comprises the following steps: checking the maturity of parent fish, collecting spermatic fluid, collecting fish roes, and carrying out artificial fertilization. Through the artificial fertilization method disclosed by the invention, the rate of fertilization is up to over 90%, and the fertilization of sperms and fish roes can be judged by naked eyes; and the unfertilized fish roes are removed, so as to greatly improve hatching rate. The artificial fertilization method effectively combines roe collection by squeezing and pressing and roe collection by in vivo manual incision, and is better than any one individual method. The disinfected parent fish of sturgeon can be re-placed into an incubation pond, and can regain health and recover ovulation capacity quickly.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, and more specifically, the invention relates to an artificial insemination method for sturgeon. Background technique [0002] At present, domestic sturgeon artificial insemination technology is not very mature, especially many aquaculture enterprises in the south do not have artificial insemination technology, and directly import fertilized eggs from Russia, Heilongjiang and other places. The Chinese invention patent with the application number 200710070446.1 discloses a Siberian sturgeon artificial propagation technology that is not affected by seasonal and geographical restrictions. After the eggs, carry out artificial insemination, then pour the fertilized eggs into a basin filled with clear water, stir evenly for 3-5 minutes, pour out the water containing semen, and wash it several times with clear water. In this invention, the sexual maturity level of the parent fish depends entirely on...

AI Technical Summary

Problems solved by technology

In this invention, the sexual maturity level of the parent fish depends entirely on the judgment of the technical personnel, and it is difficult for relatively unskilled technical personnel to grasp the degree of sexual maturity; moreover, in the process of artificial insemination, the length of the stirring time It is directly related to the level of fertilization rate. If the stirring time is too short, the fertilization is insufficient, resulting in a low fertilization rate. In this invention, stirring for 3-5 minutes, the time is too short, the fertilization is insufficient, and the fertilization rate is not high; in addition, the invention also does not work well for debonding

[0003] There are also many literatures reporting the artificial insemination technology of sturgeon, but there are some problems in the artificial insemination technology of sturgeon reported in these literatures: 1. There is no effective combination of the extrusion method and the live artificial laparotomy

2. Penicillin is generally used to disinfect the sturgeon after laparotomy, which will gradually reduce the immunity of the fish. As time goes by, the number of laparotomy increases and the survival rate of broodstock will decrease.

3. There is no simple and feasible method to judge whether fish eggs are fertilized