The invention relates to a grain refining method of high-strength long-service-life gear steel. The grain refining method of the high-strength long-service-life gear steel comprises the following steps of controlling the steel 0. 020%-0.030% of Al, 0.010%-0.060% of Nb, 0.0060%-0.0100% of N, Ti is less than or equal to 0.035%, O is less than or equal to 0.012%, the content of Al and Ti is reduced,Nb is increased, a part of N is matched, and the crystal grains are refined by the co-action of Al+Nb+N. The rolling process has a key influence on grain refinement, in the aspect of rolling, according to the melting of AlN, NbC, NbN, Nb (C, N), the research on the precipitation temperature and the precipitation phase is improved in a targeted manner, during heating, Nb, C, Al and N are fused intogamma-Fe, a large compression ratio is adopted for the first several times of rolling, so that large deformation is formed; the tissue is refined, tissue preparation is provided for separation of theposterior particle AlN and NbC; the final rolling temperature is controlled, so that a large amount of particles such as NbC, AlN and the like after rolling are separated out; after rolling, slow cooling is carried out, the cooling speed is less than or equal to 8 DEG c./ min, the precipitation time of Al, Nb carbide, nitride or carbonitride is prolonged, through the above improvement, the grainsize of the gear steel is ensured to be fully refined.