Animal feed additive and animal feed
A feed additive, feed technology, applied in animal feed, animal feed, medical raw materials derived from bacteria, etc., can solve problems such as antibacterial research
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[0082] [I] Evaluation of antibacterial properties
[0083] Production of MEL
[0084] (1) Culture of Pseudoaspergillus yeast
[0085] (pre-culture)
[0086] Put 10ml of potato dextrose medium into the test tube and stop it with a silicon stopper. After being sterilized by an autoclave, Pseudozyma aphidis NBRC 10182 was inoculated, and cultured with shaking at 30° C. for 24 hours.
[0087] (Main training)
[0088] Will contain ion-exchanged water, soybean oil 8%, NaNO 3 0.2%, KH 2 PO 4 0.02%, MgSO 4 ·7H 2 50 ml of a medium containing 0.02% O and 0.1% yeast extract was put into a 500 ml Erlenmeyer flask, blocked with a silicon stopper, and sterilized with an autoclave. The above-mentioned pre-culture solution of NBRC10182 was added thereto, and shaken at 30° C. / 220 rpm for 7 days.
[0089] (2) Extraction and purification of MEL
[0090] (extraction)
[0091] Use a separatory funnel to take 50 ml of the culture solution obtained in the main culture, extract twice wi...
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