Application of rhamnolipid as additive

A rhamnolipid and adjuvant technology, applied in the application, biocide, animal repellent and other directions, can solve the problems of large processing workload, inability to effectively deal with oily sludge, difficult to settle, etc., to reduce human body and environment. The harm of the plant, the effect of inhibiting plant fungal diseases, and reducing the amount of sludge treated

Active Publication Date: 2013-05-01
浙江圣达紫金生物科技有限公司
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  • Description
  • Claims
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Problems solved by technology

[0009] In addition, the organic matter in the oily sludge of petrochemical enterprises is complex and has different components, which has an unpleasant smell and is easy to pollute the environment. It is easy to form a suspended emulsion with various impurities such as sediment, which has a high viscosity and is difficult to settle, resulting in a huge processing workload.
Existing wastewater and sludge treatment methods cannot effectively treat oily sludge, causing serious pollution to the environment

Method used

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  • Application of rhamnolipid as additive
  • Application of rhamnolipid as additive
  • Application of rhamnolipid as additive

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Experimental program
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Effect test

preparation example Construction

[0038] The preparation method of this rhamnolipid, concrete steps are as follows:

[0039] 1. Strain cultivation: inoculate the Pseudomonas aeruginosa ZJU-211 strain into the shake flask seed medium for cultivation, the temperature is 32-38 oC , the rotating speed of the shaker is 120-200rpm, and the strain is obtained by culturing for 24-48 hours.

[0040] The composition of the shake flask seed medium (g / L): waste oil, 30-40: NaNO 3 , 2~4; NaCl, 0.5~1.5; KCl, 0.5~1.5; CaCl 2 2H 2 O, 0.05~0.2; KH 2 PO 4 , 2~4; Na 2 HPO 4 12H 2 O, 2~4; MgSO 4 , 0.1~0.3; trace elements, 1.5~2.5 mL / L, the balance is water.

[0041] Trace element composition (g / L): FeCl 3 ·6H 2 O 0.01~0.08, ZnSO 4 ·7H 2 O 0.1~0.75, CuSO 4 ·5H 2 O 0.01~0.075, MnSO 4 ·H 2 O 0.1~0.75, H 3 BO 3 , 0.01~0.15, the balance is water.

[0042] 2. Fermentation: inoculate the shake flask strain into a fermenter of 10L or its integral multiple, and cultivate it with the fermentation medium. oC , the ven...

Embodiment 1

[0056] Embodiment 1: the preparation of debacterial rhamnolipid

[0057] 1. Strain: the fermentation strain is Pseudomonas aeruginosa ZJU-211 ( Pseudomonas aeruginosa ZJU-211)

[0058] 2. Inoculate the strain on the seed medium at a temperature of 35 o C, the rotating speed of the shaker is 180rpm, and cultured for 48h to obtain the shake flask strain.

[0059] The composition of the seed medium is as follows (g / L):

[0060] McDonald's Waste Grease, 30: NaNO 3 , 2; NaCl, 0.5; KCl, 0.5; CaCl 2 2H 2 O, 0.05; KH 2 PO 4 , 2; Na 2 HPO 4 12H 2 O,2; MgSO 4 , 0.1; trace elements, 1.5 mL / L, the balance is water. Described trace element composition is as follows: FeCl 3 ·6H 2 O 0.01, ZnSO 4 ·7H2 O 0.1, CuSO 4 ·5H 2 O 0.01, MnSO 4 ·H 2 O 0.1 g / L, H 3 BO 3 , 0.01, and the balance is water.

[0061] 3. Production of rhamnolipid by fermentation in 10L fermenter

[0062] Inoculate the shake flask strain into a 10L fermenter with an inoculation amount of 5% (v / v), and...

Embodiment 2

[0067] Embodiment 2: the preparation of rhamnolipid concentrate

[0068] 1. Strain: the fermentation strain is Pseudomonas aeruginosa ZJU-211 ( Pseudomonas aeruginosa ZJU-211)

[0069] 2. Inoculate the strain on the seed medium at a temperature of 38 o C, the rotation speed of the shaker is 200rpm, and cultured for 48h to obtain the shake flask strain.

[0070] The composition of the seed medium is as follows:

[0071] Rapeseed oil processing waste, 40 g / L: NaNO 3 , 4 g / L; NaCl, 1.5 g / L; KCl, 1.5 g / L; CaCl 2 2H 2 O, 0.2 g / L; KH 2 PO 4 , 4 g / L; Na 2 HPO 4 12H 2 O, 4 g / L; MgSO 4 , 0.3 g / L; trace elements, 2.5 mL / L, the balance is water. Described trace element composition is as follows: FeCl 3 ·6H 2 O 0.08 g / L, ZnSO 4 ·7H 2 O 0.75 g / L, CuSO 4 ·5H 2 O 0.075 g / L, MnSO 4 ·H 2 O 0.75 g / L, H 3 BO 3 , 0.15 g / L, and the balance is water.

[0072] 3. Production of rhamnolipid by fermentation in 50L fermenter

[0073] Inoculate the shake flask strain into a 50L ...

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Abstract

The present invention discloses an application of rhamnolipid as an additive. According to the present invention, the rhamnolipid is adopted as a pesticide additive so as to significantly improve hydrophilicity and provide temperature stability and chemical environment stability; the rhamnolipid is adopted as a fertilizer additive, such that the foliar fertilizer has effects of significant nutrient uptake promotion and plant growth promotion; and the rhamnolipid is adopted as a feed additive, such that emulsification of various nutrients in food and small intestine nutrient absorption can be promoted. With the present invention, the rhamnolipid is adopted to replace a chemical surfactant to be adopted as the additive of the pesticide, the fertilizer and the feed additive so as to provide significant effects.

Description

[0001] This application is a divisional application of a patent application with an application date of March 30, 2010, an application number of 201010136140.3, and an invention title of "A Preparation Method and Application of Rhamnolipid". technical field [0002] The invention relates to the technical field of biosurfactants, in particular to the application of a rhamnolipid as an auxiliary agent. Background technique [0003] Biosurfactants are a class of surfactants produced by microorganisms, animals or plants, which are very similar in performance to chemically synthesized surfactants, that is, the molecules are composed of polar hydrophilic groups and non-polar lipophilic groups. A molecular layer is formed on the phase interface to reduce the energy of the interface, and it has good surface activity, such as emulsification, dispersion, wetting and penetration. Compared with chemically synthesized surfactants, biosurfactants have stronger surface activity and stronge...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/30C05G3/00A23K1/16
Inventor 孟琴张国亮
Owner 浙江圣达紫金生物科技有限公司
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