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Method for enhancing yield of rhamnolipid produced by copper green pseudomonas

A technology of Pseudomonas aeruginosa and rhamnolipid, which is applied in the field of increasing the yield of rhamnolipid, can solve the problems of high production cost of biosurfactants and the like, and achieves the improvement of composting microenvironment, easy price, and increase of rhamnose The effect of lipid production

Inactive Publication Date: 2008-05-21
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the expensive production cost of biosurfactants has become a bottleneck for their widespread adoption in industry

Method used

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  • Method for enhancing yield of rhamnolipid produced by copper green pseudomonas
  • Method for enhancing yield of rhamnolipid produced by copper green pseudomonas

Examples

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Embodiment 1

[0017] The bacterial classification used in the present invention is Pseudomonas aeruginosa (numbering Pseudomonas aeruginosa CCTCCAB93066), and this bacterial classification is purchased from China Typical Culture Collection Center, is inoculated once on the slant medium every 1 to 2 months, and bacterial classification is in 4 Store under ℃ conditions.

[0018] 1. Take the refrigerated strains and activate them at 37°C for 24 hours, and then use an inoculation loop to pick a ring of strains from the slant culture medium in the aseptic operating table and inoculate them in the enriched culture medium. Sterilize in a pot at 115°C for 30 minutes. The inoculated enriched culture solution was cultured in a constant temperature shaking incubator at 37° C. and 200 rpm. The composition of the enriched culture medium is: NaCl, 5.0g; peptone, 5.0g; beef powder, 3.0g; distilled water, 1000mL; and adjust the pH to 7.0 with 1M NaOH.

[0019] 2. Inoculate the enriched culture solution o...

Embodiment 2

[0023] 1. As in Example 1, take the strains refrigerated at 4°C and activate them at 30°C for 24 hours, then use an inoculation loop to pick a ring of strains from the slant culture medium in the aseptic operating table and inoculate them in the enriched culture solution The enriched culture solution was sterilized in a high-pressure steam boiler at 115°C for 30 minutes in advance, and the inoculated enriched culture solution was placed in a constant temperature shaking incubator at 30°C and 250rpm. The composition of the enriched culture solution is: NaCl, 4.5g; peptone, 4.5g; beef powder, 2.5g; distilled water, 1000mL; and adjust the pH to 7.2 with 1M NaOH.

[0024] 2. Inoculate the enriched culture solution cultured for 24 hours into the fermentation culture solution at an inoculum size of 5% by volume. The composition of the fermentation medium is: glucose, 25g; NaNO 3 , 2.3g; KH 2 PO 4 , 1.8g; Na 2 HPO 4 12H 2 O, 1.8g; MgSO 4 ·7H 2O, 0.8g; FeSO 4 ·7H 2 O, 0.015g...

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Abstract

The invention relates to a method for increasing the rhamnolipid with pseudomonas aeruginosa; the method comprises steps of the activation and enrichment of the pseudomonas aeruginosa, aerobic fermentation culture, high-speed centrifugal sterilization, extraction and air drying of the fermentation liquid. The invention, with added vector, enlarges the accretion of the pseudomonas aeruginosa to increase the output of the rhamnolipid, which is beneficial to the management of environmental pollution.

Description

technical field [0001] The invention relates to a method for increasing the production of rhamnolipids. Background technique [0002] Biosurfactants have gradually been paid attention to because of their structural diversity, non-toxic or low-toxicity, and biodegradability, and are gradually replacing chemically synthesized surfactants in some aspects. In the field of municipal solid waste composting, biosurfactants can improve the microenvironment of composting, thereby promoting the decomposition of organic waste by microorganisms and improving the treatment efficiency of waste in composting. However, the expensive production cost of biosurfactants has become a bottleneck for their widespread adoption in industry. If the production cost of biosurfactant can be reduced, or its output can be increased under the same production cost, its application in industrial and environmental pollution control will be more extensive. [0003] Previous literature mentioned that when Bac...

Claims

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Application Information

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IPC IPC(8): C12P19/02C12R1/385
Inventor 刘小兰曾光明钟华傅海燕刘智峰陈耀宁黄海翼王静
Owner HUNAN UNIV
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