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Immobilized microorganism particle for degrading petroleum in water

A technology of immobilized microorganisms and PetroChina, which is applied in the direction of water pollutants, biological water/sewage treatment, immobilized on/in organic carriers, etc., and can solve the problems of restricting microbial degradation speed and processing time, and poor degradation effect. Achieve the effects of increasing availability, reducing interfacial tension, and improving degreasing effect

Inactive Publication Date: 2013-04-10
SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The dispersibility, droplet size, and surface tension of oil pollutants in water are the main factors affecting their diffusion in the embedding carrier, which seriously restricts the microbial degradation rate and treatment time of such pollutants, even in In the case of high concentration of dominant bacteria, the degradation effect is still poor

Method used

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  • Immobilized microorganism particle for degrading petroleum in water

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] 1. Preparation of immobilized microbial particles

[0014] The components and proportions of the immobilized microbial particles are shown in Table 1.

[0015] Table 1 The proportion of each component of immobilized microbial particles

[0016] Polyvinyl alcohol (W / V)

[0017] Preparation method, concrete steps are as follows:

[0018] 1) Treatment of activated sludge: The activated sludge is taken from the return sludge of a domestic sewage treatment plant in Shenyang, and domesticated through the laboratory activated sludge method simulation device. The domestication conditions are: sludge concentration 3g / L, DO3~4mg / L, HRT 3h. After acclimatization for 30 days, the activated sludge in the logarithmic growth phase was centrifuged at 3500r / min for 15min, and the supernatant was discarded to take the precipitate for later use.

[0019] 2) Prepare the embedding material mixture according to the ratio and composition in Table 1, and set aside;

[0020] 3) M...

Embodiment 2

[0029] 1. Preparation of immobilized microbial particles

[0030] For the immobilized microbial particles, the components and proportions of the embedding mixture are shown in Table 3.

[0031] Table 3 Ratio of each component of immobilized microbial particles

[0032]

[0033] Preparation method, concrete steps are as follows:

[0034] 1) Treatment of activated sludge: The activated sludge is taken from the return sludge of a domestic sewage treatment plant in Shenyang, and domesticated through the laboratory activated sludge method simulation device. The domestication conditions are: sludge concentration 3g / L, DO 3~4mg / L, HRT 3h. After acclimatization for 30 days, the activated sludge in the logarithmic growth phase was centrifuged at 3500r / min for 15min, and the supernatant was discarded to take the precipitate for later use.

[0035] 2) Prepare the embedding material mixture according to the ratio and composition in Table 3, and set aside;

[0036] 3) Mix the preci...

Embodiment 3

[0045] The immobilized microbial particles are composed of embedding material and activated sludge, wherein the embedding material and activated sludge are mixed at a mass ratio of 2:1; the embedding material is composed of carrier, SiO 2 , surfactant and crosslinking agent, the carrier is polyvinyl alcohol and sodium alginate; the surfactant is Tween 80, and the carrier concentration is 10% (W / V), SiO 2 The concentration is 2% (W / V), and the final concentration of Tween80 in the surfactant is 150 mg / L.

[0046] The carrier is polyvinyl alcohol and sodium alginate in a mass ratio of 15:1.

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Abstract

The invention belongs to the technical field of biological immobilization and environmental protection and particularly relates to an immobilized microorganism particle for degrading petroleum in low-concentration oily water. The immobilized microorganism particle consists of embedding materials and activated sludge, wherein the embedding materials and the activated sludge are mixed according to a mass ratio being 0.5-2:1, the embedding materials consist of carriers, SiO2, surface active agents and cross-linking agents, the carriers are polyvinyl alcohol and sodium alginate, and the surface active agents are Tween 80 and / or rhamnolipid, wherein the carrier concentration is 8 percent to 12 percent (W / V), the SiO2 concentration is 1.5 percent to 3 percent (W / V), the final concentration of the Tween 80 in the surface active agents is 100 to 200mg / L, and the final concentration of the rhamnolipid is 100 to 180mg / L. The immobilized microorganism particle prepared by the method has the advantages that the interface tension can be effectively reduced, the oil / water interface area is increased, the biological degradation processes of oil pollutants are obviously promoted, and the deoiling effect of microorganisms is greatly improved.

Description

technical field [0001] The invention belongs to the technical field of biological immobilization and environmental protection, in particular to an immobilized microbial particle for degrading oil in water. Background technique [0002] A large amount of petroleum pollutants will be produced in the process of oil exploitation and production. These pollutants can enter the surface water body through various channels, causing a certain degree of pollution to the local surface water system, and the pollution presents a large area of ​​pollution, low concentration of pollutants, and is difficult to remove. Features. In addition, the surface water body has high fluidity, strong seasonality, and complex pollution characteristics that increase the difficulty of implementing traditional remediation technologies. [0003] Immobilized microorganism technology is a basic technology that uses chemical or physical means to locate free cells or enzymes in a limited space area to keep them...

Claims

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Application Information

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IPC IPC(8): C12N11/10C12N11/08C12N11/04C02F3/34C02F101/32
Inventor 郭书海魏小娜李刚吴波
Owner SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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