The invention discloses an ultrasonic positioning sampling puncture system which comprises an ultrasonic probe, the right side of the ultrasonic probe is fixedly connected with an externally-hung fixed end, a handle is arranged at the upper end of the ultrasonic probe, a control switch is arranged on one side of the handle, a connecting body is arranged outside the externally-hung fixed end and fixedly connected with a rotating frame, and a rotating shaft is arranged in the center of the rotating frame. The right side of the sliding rail is fixedly connected with a moving seat, the right side of the moving seat is fixedly connected with a sampling puncture needle, the sampling puncture needle comprises a needle tube, the front end of the needle tube is movably connected with a puncture needle body, and the puncture needle body is pushed to move on the rotating frame in the length direction of the rotating frame; a grabbing mechanism is arranged in the puncture needle body and comprises a grabbing hand and a grabbing hand connecting pipe, the advantages of being high in target accuracy, good in safety and easy to operate can be achieved, and under the assistance of a computer, the target can be rapidly determined, the positioning process is simplified, the operation difficulty is lowered, dependence on the experience of an operator is reduced, and the working efficiency is improved.