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Formula for culture medium of cordyceps militaris liquid strains and method for culturing same

A medium formula and liquid strain technology, which is applied in botanical equipment and methods, fertilizer mixtures, horticulture, etc., can solve the problems of difficult to observe the growth of mycelium, low transparency, turbid medium, etc., so as to facilitate the observation of bacteria. The effect of silk growth condition, lower production cost and convenient production

Inactive Publication Date: 2009-08-26
镇江市食用菌研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to overcome the problems of turbidity, low transparency, difficulty in observing the growth of mycelium and the use of equipment such as fermentation tanks and shakers in the existing liquid culture medium, the investment is large, the operation is cumbersome, and it is not suitable for ordinary people's family production. The present invention discloses a The formula and cultivation method of Cordyceps militaris liquid culture medium, the medium is not only suitable for the growth of Cordyceps militaris mycelia, but also clear and transparent, easy to observe the growth of mycelium, can detect and deal with polluted bacteria in time, and reduce the pollution caused by bacteria Unnecessary losses caused by inoculation can improve the success rate of Cordyceps militaris cultivation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1. Prepare 14 washed 500ml cans, with a side length of 12cm, a square polypropylene film with a thickness of 5 wires, and an appropriate amount of rubber bands.

[0017] 2. Prepare medium: first add vitamin B 1 6 tablets (60 mg), crushed and dissolved in water, weighed 60 grams of glucose, 3 grams of ammonium citrate, 3 grams of magnesium sulfate, 3 grams of potassium dihydrogen phosphate, dissolved in water, and then dissolved 24 grams of peptone in hot water Then mix it with the aqueous solution of other raw materials, filter through 6-8 layers of gauze, add water to make up to 3000 ml, and finally adjust the pH value to 7.5 with aqueous sodium hydroxide solution.

[0018] 3. Sterilization by bottling: put the prepared culture solution into canned bottles with an amount of 200-250 ml per bottle, seal it with the prepared polypropylene film and rubber band, and put it into a medical portable pressure cooker for sterilization (Can only hold 14 canned bottles), accordin...

Embodiment 2

[0024] 1. Wash the 500ml triangular flask or saline bottle. Determine the number of production bottles according to the required amount of the strain, and then determine the quantity of culture solution according to the number of production bottles, 200-250ml per bottle.

[0025] 2. Preparation of culture medium: Add 8 grams of peptone, 20 grams of glucose, 1 gram of ammonium citrate, 1 gram of magnesium sulfate, 1 gram of potassium dihydrogen phosphate, and vitamin B to 1000 ml of culture solution. 1 Weigh out the required raw materials in proportion to 20 mg. Vitamin B 1 Dissolve in water after crushing, then dissolve glucose, ammonium citrate, magnesium sulfate, and potassium dihydrogen phosphate in water, then dissolve peptone in hot water and mix with the aqueous solution of other raw materials, filter through 6-8 layers of gauze and add water to make up to the amount of culture solution that needs to be produced, and finally adjust the pH value to 7.5 with aqueous sodi...

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Abstract

The invention discloses a formula for the culture medium of cordyceps militaris liquid strains and a method for culturing the same. The invention is characterized in that the method comprises the following steps: preparing a culture solution with the pH value being 7.5 from the following components by percentage: 0.8% of peptone, 2% of glucose, 0.1% of ammonium citrate, 0.1% of magnesium sulfate, 0.1% of monopotassium phosphate, 0.002% of vitamin B1 and 92.9% of water; filling containers, such as erlenmeyer flasks, saline bottles or preserving jars, with the culture solution; carrying out regular autoclaving on the culture solution; then, grafting the slant mother culture of cordyceps militaris onto the culture solution; and carrying out standing and culturing for 7 to 10 days in a light-blocking and airtight culture room with the temperature being 18 to 22 DEG C to obtain the cordyceps militaris liquid strains. The invention dispenses with fermentation tanks, shaking tables and other devices, therefore, the investment is small; no sterile air is introduced, therefore, the operation is convenient; and no energy is consumed, as a result, the invention is energy-saving and environment-friendly.

Description

technical field [0001] The invention relates to a formula and a cultivation method of a liquid culture medium of Cordyceps militaris, belonging to the technical field of edible fungus production. Background technique [0002] Cordyceps militaris, also known as Cordyceps militaris, is known as the "Holy Grass of the East". It is a precious Chinese medicinal material with high medical care and nourishing value. Many medicinal ingredients contained in Cordyceps militaris are close to or exceed those of Cordyceps sinensis, and have similar miraculous efficacy to Cordyceps sinensis. After the success of artificial cultivation of Cordyceps militaris, it has developed rapidly in recent years. The output of Cordyceps militaris has increased rapidly, the price has dropped significantly, and the market consumption has also increased accordingly. There is a trend of developing from medicine to food. In 2008, the Ministry of Health has approved Cordyceps militaris as a new resource foo...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05G3/00
Inventor 朱忠贵
Owner 镇江市食用菌研究所
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