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Biocontrol strain KMXU1 capable of preventing and treating blueberry blight and antibiological inoculant thereof

A technology for bio-control strains and bio-control agents, applied in the directions of biocides, fungicides, biocides, etc., can solve the problems of high cost, difficulty, and high chemical pesticide residues, and achieve growth inhibition, increase microbial activity, and protection. Effects of farmland ecosystems

Inactive Publication Date: 2013-04-03
KUNMING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0020] The object of the present invention is to provide a kind of biocontrol bacterial strain KMXY1 and its biological control strain KMXY1 for preventing and treating blueberry wilt in order to solve the problems that the prevention and treatment of blueberry wilt is difficult and costly in organic cultivation, and chemical pesticides and pesticide residues are not suitable for use in current agricultural production. antibacterial agent

Method used

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  • Biocontrol strain KMXU1 capable of preventing and treating blueberry blight and antibiological inoculant thereof
  • Biocontrol strain KMXU1 capable of preventing and treating blueberry blight and antibiological inoculant thereof
  • Biocontrol strain KMXU1 capable of preventing and treating blueberry blight and antibiological inoculant thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1: the preparation of biocontrol agent

[0037] a. Transplant the strain KMXU1 into a test tube for one-ring activation, and incubate at 28°C for 48 hours; the PSA liquid medium in the test tube is: Weigh 200g of potatoes, wash, peel and chop them, add 1000ml of water to boil for half an hour, filter with gauze , add 20g of ordinary sucrose and 17g of agar, fully dissolve, filter with gauze while hot, and sterilize with 15 pounds of steam (121°C) for 20 minutes;

[0038] b. Take one ring of activated bacterial lawn and transfer it into 600ml 1000ml Erlenmeyer flask with formula: tryptone 10g / L, yeast extract 5g / L, sodium chloride 5g / L, pH7.0 LB culture medium, Cultivate at 120 rpm and 28°C for 72 hours to obtain fermentation broth;

[0039] c. Inoculate the fermentation broth into the same LB broth as in step b in the seed tank at a ratio of 1:200 for inoculation, and ferment for 16 or 17 hours at the temperature of the seed tank at 29°C or 30°C or 31°C Or ...

Embodiment 2

[0042] Embodiment 2: plate antagonism test

[0043] According to Fang Zhongda's "Plant Disease Research Methods" plate-supported culture test method, the inhibitory effect of KMXY1 bacteria on blueberry Fusarium wilt was evaluated. At the same time, the sterilized distilled water control treatment group had the following statistical results when the mycelia covered the plate. The growth inhibition rate of KMXY1 against Fusarium wilt was 90.00% (Table 1). It shows that the KMXY1 strain has a significant inhibitory effect on blueberry wilt.

[0044] Table 1 Inhibitory effect of KMXY1 on blueberry Fusarium wilt and related pathogens

[0045]

Embodiment 3

[0046] Embodiment 3: Greenhouse Control Efficiency Test of Blueberry Fusarium Wilt

[0047] Three treatments were set up, treatment 1 was the sterile water control group, treatment 2 was the KMXY1 bacterial agent dilution, and treatment 3 was the fungicide carbendazim (Nantong Runhong Biochemical Co., Ltd.). Blueberry annual tissue-cultured seedlings are transplanted into blueberry Fusarium wilt diseased soil. When transplanting, dip the roots with KMXY1 bacterial agent 1:50 times, and transplant while dipping the roots; 7-10 days after the crops are transplanted, use KMXY1 bacterial agent 1: Irrigate the roots with 50 times of bacterial solution, and irrigate the roots of 5-6 crops per kilogram of liquid; irrigate the roots with KMXY1 bacterial agent 1: 50 times of bacterial solution, and irrigate the roots of 5-6 crops per kilogram of liquid. Other clear water controls and carbendazim pesticide treatment were the same as the above method and dosage. The amount of the prepar...

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Abstract

The invention relates to a biocontrol strain KMXU1 capable of preventing and treating blueberry blight and antibiological inoculant thereof and belongs to the technical field of biopesticide. Preservation number of the strain KMXU1 is CGMCC NO. 6597. The antibiological inoculant is obtained by the following steps of: transplanting the strain KMXU1 into a test tube, carrying out one-cycle activation, and culturing for 48 hours at the temperature of 28 DEG C; transferring an activated bacterial lawn into 600ml of LB culture solution filled in a 1000ml conical flask, and culturing for 72 hours under the conditions that the rotating speed is 120r / min and the temperature is 28 DEG C, so that zymogeneous bacteria solution is obtained; inoculating the zymogeneous bacteria solution into the LB culture solution in a seed tank in the ratio of 1: 200 by inoculation amount, carrying out fermental cultivation under the condition that temperature of the seed tank is 29-31 DEG C, and fermenting for 16-18 hours, so that strains used for fermentation is obtained; inoculating the strains used for fermentation into a fermentation tank in the ratio of 1: 20 by the inoculation amount, carrying out the fermental cultivation under the condition that the temperature of the fermentation tank is 29-31 DEG C, fermenting for 72-96 hours, and storing the fermented strains as the antibiological inoculant; and centrifuging the antibiological inoculant at 5000rpm for 6 min, and preparing active inoculant with preservative fluid in the ratio of 1: 50 (g / ml). The invention has the advantages that cost is low, no position or pollution is produced, and biocontrol effect is good; and successive cropping obstacle of blueberry soil-borne disease can be effectively prevented, and fruit yield of blueberry is increased.

Description

Technical field: [0001] The invention relates to a biocontrol bacterial strain KMXY1 for preventing and treating blueberry wilt and a biocontrol bacterial agent thereof, belonging to the technical field of biological pesticides. Background technique: [0002] Blueberry (Vaccinium spp.), is a kind of perennial small berry fruit tree belonging to Ericaceae (Ericaceae) Vaccinium (Vaccinium), widely used in medicine, health care, cosmetics and environmental protection, etc., the United Nations Food and Agriculture Organization will Its fruit is listed as one of the top five healthy foods in the human world [1, 2], and it is also an economic crop worthy of development in the southern acid soil hilly area. According to statistics, by 2008, more than 10 provinces across the country have started large-scale commercial cultivation, and the total cultivation area has increased from 24 hm2 in 2000. 2 Rapid development to more than 2000 hm 2 , it is expected that in the next 10 years...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
Inventor 余磊赵建荣
Owner KUNMING UNIV
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