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New technique for producing lactic acid through solid state fermenting dregs of potato by rhizopus of rice

A technology of solid-state fermentation and Rhizopus oryzae, applied in the directions of fermentation, microorganisms, microorganism-based methods, etc., can solve the problems such as the production of lactic acid by solid-state fermentation of potato residues of Rhizopus oryzae, which can save energy consumption, reduce production costs, The effect of promoting reproduction and accumulation of lactic acid

Inactive Publication Date: 2007-10-10
HEFEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there has been no report on the production of lactic acid by solid-state fermentation of potato residue by Rhizopus oryzae

Method used

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  • New technique for producing lactic acid through solid state fermenting dregs of potato by rhizopus of rice
  • New technique for producing lactic acid through solid state fermenting dregs of potato by rhizopus of rice
  • New technique for producing lactic acid through solid state fermenting dregs of potato by rhizopus of rice

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0041] Rhizopus oryzae PW352 slant seed culture: culture at 36°C for 3 days; solid-state fermentation culture: routine high-temperature sterilization of the medium, inoculation of 1-2ml spore suspension (1×10 7 spores / ml), stirred evenly, and cultivated at 34°C for 3 days, and the fermentation vessel was a 250ml Erlenmeyer flask.

[0042] The solid-state fermentation medium consists of: 10 g of sweet potato residue, and the weight percentages of all the other components in sweet potato residue are as follows: KH 2 PO 4 0%, MgSO 4 0.60%, ZnSO 4 0.15%, MnCl 2 0.15%, calcium carbonate addition 20%, glucose 1.0%, nitrogen source selection 2.0% (NH 4 ) 2 SO 4 , Tween 80 0.10%, add 66-70% water of the total weight of the above components, and the pH is natural.

[0043] The amount of L-lactic acid produced after 72 hours of fermentation reached 31.71 (±0.72)% (w / w) of the weight of sweet potato residue, and the conversion rate of starch sugar was 57.38% (±1.30).

example 2

[0045] Seed preparation and solid-state fermentation conditions are the same as Example 1, and the fermentation container is a 250ml Erlenmeyer flask.

[0046] The components of the solid-state fermentation medium are: sweet potato residue 10g, ZnSO 4 0.20%, MgSO 4 0.80%, MnCl 2 0.20%, calcium carbonate added 20%, glucose 0.50%, (NH 4 ) 2 SO 4 2.5%, Tween 80 0.15%, add 70% water of the total weight of the above components, and the pH is natural.

[0047] The amount of L-lactic acid produced by fermentation for 72 hours reached 28.74 (±0.48)% (w / w) of the weight of sweet potato residue, and the conversion rate of starch sugar was 52.01%.

example 3

[0049] Seed preparation and solid-state fermentation conditions are the same as Example 1, and the fermentation container is a 250ml Erlenmeyer flask.

[0050] The components of the solid-state fermentation medium are: sweet potato residue 10g, KH 2 PO 4 0.4%, ZnSO 4 0.1%, MgSO 4 0.8%, MnCl 2 0%, calcium carbonate addition 20%, glucose 0.5%, (NH 4 ) 2 SO 4 2.0%, Tween 80 0.20%, add 70% water of the total weight of the above components, the pH is natural.

[0051] The amount of L-lactic acid produced by fermentation for 72 hours reached 24.77 (±0.43)% (w / w) of the weight of sweet potato residue, and the conversion rate of starch sugar was 44.82% (±0.78).

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Abstract

This invention relates to a method for producing L-lactic acid from Rhizopus oryzae by solid-state fermentation. The method comprises: (1) culturing the seeds in an inclined culture medium at 30-36 deg.C for 3-5 days, inoculating into an erlenmeyer flask or eggplant flask of sporulation culture medium, culturing at 28-36 deg.C for 70-90 h, and expanding culture according to the inoculation amount of the seeds to obtain Rhizopus oryzae spores with corresponding amount; (2) inoculating Rhizopus oryzae seeds into a fermentation tank loaded with 15-60 vol.% of solid-state fermentation culture medium, and culturing at 28-36 deg.C for 48-84 h. The himidity is 65-90%, and the aeration rate of the fermentation tank is 10-20 L / h. Compared with liquid-state fermentation, the solid-state fermentation has such advantages as no need for stirring, low energy consumption, low production cost, and high L-lactic acid quality.

Description

technical field [0001] The invention belongs to the technical field of organic acid production, in particular to a production method of lactic acid. Background technique [0002] Lactic acid is an important organic acid that widely exists in the human body, animals, plants and microorganisms. Lactic acid is one of the three most recognized organic acids in the world. It is a multi-purpose biochemical product. Lactic acid, lactic acid salts and their derivatives are widely used in food, medicine, feed, chemical industry, environmental protection, agriculture and other fields. In particular, polylactic acid (PLA) produced with L-lactic acid, in addition to having similar gloss and processing properties to polystyrene, is also biodegradable and can be used to produce biodegradable plastics, which are formed by human long-term use of plastic products. It provides a solution to the problem that waste cannot be treated, so its application prospect is very promising. [0003] The...

Claims

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Application Information

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IPC IPC(8): C12P7/56C12R1/845
Inventor 张洁潘仁瑞沈寿国刘献文蔡敬民葛春梅吴克
Owner HEFEI UNIV
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