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Bacillus subtilis for preventing and controlling Chinese herbal medicine soil-borne diseases and preparation method of bactericide thereof

A technology of Bacillus subtilis and soil-borne diseases, applied in the direction of fungicides, bacteria, biocides, etc., can solve the problems of harming non-target organisms, pesticide residue pollution, etc.

Active Publication Date: 2012-07-04
INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a kind of Bacillus subtilis for preventing and treating soil-borne diseases of Chinese herbal medicines and the preparation thereof; the purpose is to solve the problem of inducing the drug resistance of pathogenic bacteria or disease insects and causing pesticide residue pollution and damage caused by the current use of chemical pesticides to prevent and treat diseases of Chinese medicinal materials. Problems with non-target organisms

Method used

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  • Bacillus subtilis for preventing and controlling Chinese herbal medicine soil-borne diseases and preparation method of bactericide thereof

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specific Embodiment approach 1

[0012] Specific embodiment one: the Bacillus subtilis for preventing and treating soil-borne diseases of Chinese herbal medicine in this embodiment is Bacillus subtilis X1 for preventing and treating soil-borne diseases of Chinese herbal medicine, which is Bacillus subtilis and belongs to the genus Bacillus. Preserved by the General Microorganism Center of China Microbiological Culture Collection Management Committee, the preservation number is CGMCC No.4037, and the preservation date is July 26, 2010; it is a Gram-positive bacterium, rod-shaped, 1.2 μm to 1.4 μm in length, and 1.4 μm in width. 0.6μm~0.8μm, spores, oval, no flagella, no pigmentation; milky white colonies formed on beef extract peptone medium, wrinkled, irregular edges, and ridge-like protrusions in the center of the colony.

[0013] The Bacillus subtilis used in the prevention and treatment of soil-borne diseases of Chinese herbal medicines in this embodiment is initially identified as the genus Bacillus accord...

specific Embodiment approach 2

[0017] Embodiment 2: This embodiment is different from Embodiment 1 in that the Bacillus subtilis X1 starch hydrolysis of the prevention and treatment of Chinese herbal medicine soil-borne diseases is positive, the oil hydrolysis is negative, the gelatin hydrolysis is positive, the litmus milk test is positive, and the urea hydrolysis is positive. Negative, negative hydrogen sulfide test, positive indole test, negative methyl red test, positive Vopps test, positive citrate test, positive peroxidase test, negative oxidase test, available Glucose and sucrose produce acid but not gas, the hippurate test is positive, the nitrate reduction test is positive, easy to grow, the growth pH is 6.0-8.0, and the growth temperature is 20-40°C. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0018] Specific implementation mode three: the preparation method of the bacterial preparation of Bacillus subtilis for preventing and treating soil-borne diseases of Chinese herbal medicines in this embodiment is carried out in the following steps: 1. Take the bacterial liquid of Bacillus subtilis X1 for preventing and treating soil-borne diseases of Chinese herbal medicines preserved in glycerin at -70°C, Streak inoculation on NYDA solid medium, culture in a 30°C incubator for 18-20h, pick a single colony and inoculate it in 5ml of NYD liquid medium, shake it at 30°C and a frequency of 150r / min for 18-20h, Obtain the activated bacterial liquid; 2. Take 0.1ml of the activated bacterial liquid and spread it on the eggplant bottle containing NYDA medium, and cultivate it in a 30°C incubator for 18-20 hours to obtain the activated bacterial strain; 3. Inoculate the activated bacterial strain into the In a fermenter with NYD liquid medium, ferment for 24-48 hours at a fermentation...

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Abstract

The invention relates to a bacillus subtilis for preventing and controlling Chinese herbal medicine soil-borne diseases and a preparation method of a bactericide thereof, in particular to a bacillus subtilis and a preparation method of the bactericide thereof. The bacillus subtilis X1 for controlling the Chinese herbal medicine soil-borne diseases is Bacillus with a preservation number of CGMCC No.4037 and a preservation date of 26th, July 2010. The bactericide is prepared by the following steps: inoculating an activated strain to a NYD fluid nutrient medium for enrichment culture to obtain asterilized solution with 2-4 billion / millimetre in bacterial count concentration. The bacillus subtilis X1 of the invention is a bio-control bacterial strain which is provided with high control effect on the soil-borne diseases and the bactericide can enjoy a prevention rate of 60-80% on the Chinese herbal medicine soil-borne diseases including ginseng root rot, sclerotiniose, North China fritillary sclerotiniose, Schisandra chinensis root rot, seedling blight and the like. The bactericide bio-control preparation is safe and environment-friendly and does not damage drug resistance of nontarget organisms and inductive pathogenic bacteria.

Description

technical field [0001] The invention relates to a bacillus subtilis and preparation thereof. Background technique [0002] The eight-character policy of the modernization of traditional Chinese medicine for the development of traditional medicine is: "safe, effective, stable, and controllable". The standards for measuring the quality of Chinese medicinal materials such as ginseng, flat shellfish, and Schisandra chinensis include chemical pesticides and heavy metals in addition to the active ingredients of traditional Chinese medicine. contamination by foreign pollutants. Pesticide residues and heavy metal pollution in Chinese ginseng are important factors that cause the quality of ginseng to decline, and become the main obstacle that restricts Chinese ginseng from going global. During the introduction and cultivation of ginseng, it will suffer from various diseases and insect pests. At present, it mainly relies on chemical pesticides for control. Chinese medicinal material...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/125
Inventor 李晶姜竹王玉霞张云湖张先成赵晓宇张淑梅孟利强
Owner INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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