Method for preventing and controlling red tide resulting from Karenia mikimotoi by pseudoalteromonas

Abstract

The invention discloses a method for preventing and controlling a red tide resulting from Karenia mikimotoi by pseudoalteromonas. According to the method, a pseudoalteromonas strain capable of remarkably inhibiting growth of red-tide Karenia mikimotoi is separated from a soil environment of a river estuary through a co-culture experiment; pseudoalteromonas fermentation liquor, thalli, filtrate and a fermentation culture medium are separately added into Karenia mikimotoi liquor for co-culture; 4% pseudoalteromonas fermentation liquor filtrate is a minimum inhibitory concentration; through 12h of treatment by the 4% pseudoalteromonas fermentation liquor filtrate, an algae cell cycle of the Karenia mikimotoi can be retarded at a G2 phase, and thus, the Karenia mikimotoi cannot enter a division stage; through 3h of action by 8% filtrate, the level of active oxygen in algae cells reaches the maximum, and it is observed by a fluorescent microscope that the algae cells completely present red; and through 6h of action, the level of active oxygen in the algae cells is lowered, and then, the algae cells rupture and present green yellow. The method has the beneficial effect that the red tide resulting from red-tide microalgae Karenia mikimotoi can be prevented and treated.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to pseudoalteromonas and its application in preventing and controlling red tides caused by Karenia militaris. Background technique [0002] Harmful algal blooms (HABs) are divided into freshwater blooms and marine red tides, which are an abnormal phenomenon of aquatic ecology: when domestic sewage, industrial wastewater, and agricultural wastewater containing a large amount of nutrients flow into oceans or lakes, in specific Under environmental conditions (such as ocean currents, temperature, and the degree of closure of sea areas, etc.), red tide organisms (phytoplankton, protozoa, or bacteria), and bloom organisms (cyanobacteria, green algae, or diatoms, etc.) will multiply rapidly to form algal blooms. Harmful algal blooms can damage aquatic ecosystems and pose serious threats to the aquatic environment, human health, and the economies of countries. The prevention and contr...

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Problems solved by technology

However, externally added substances will inevitably cause secondary environmental problems and have direct or indirect side effects on other organisms, such as the widely used chemical algicide CuSO 4 Not only can it accelerate the release of algal toxins, but high concentrations of copper ions will cause heavy metal pollution in water, resulting in the death of phytoplankton and aquatic animals; a large amount of clay used in physical methods is suspended in seawater or deposited on the seabed, and the amount of sludge is too large for shrimp The growth and survival of scallops have a great influence

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