Broiler feed additive and preparation method thereof
A broiler feed and additive technology, applied in the field of poultry breeding, can solve problems such as bacterial resistance
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Embodiment 1
[0011] The preparation of embodiment one Polygonum caperica extract
[0012] Materials and Methods
[0013] 1. The source and pretreatment of Polygonum sp.
[0014] Spicy Polygonum is collected from Guanghan City, Sichuan Province, China, and the whole plant is dried in the shade. Before extraction, place the shade-dried Polygonum punctatus in an oven at 60-70°C to dry for later use.
[0015] 2. Preparation of Polygonum capsicum extract
[0016] 2.1 Lab preparation of Polygonum extract. Cut the dried whole herb of Polygonum officinale into a length of about 0.2-0.5cm, then weigh 100g, add 0.5L of distilled water, soak for 30 minutes, boil in an open container, and decoct with low heat for 15 minutes. Use 4 layers of gauze to filter the medicinal solution; add 0.3L of water to the remaining medicinal residues, boil and simmer for 15 minutes, filter the medicinal solution with 4 layers of gauze; then add 0.2L of water to the remaining medicinal residues, boil and simmer for ...
Embodiment 2
[0019] The bacteriostatic test of embodiment two peppery Polygonum extracts to typical pathogenic bacteria of chicken intestinal tract in vitro
[0020] Polygonum is usually used as a medicine for treating intestinal diseases in the folk pharmacopoeia, indicating that the active ingredients in it may inhibit or kill the growth of intestinal microorganisms. Therefore, this experiment selected three typical enteric pathogenic bacteria as representatives, and investigated whether the extract of Polygonum punctatus had any effect on these bacteria in vitro.
[0021] 1. Materials and methods
[0022] 1 strain
[0023] Staphylococcus aureus; Escherichia coli ATCC25922; Salmonella pullorum. The above strains were provided by the Department of Poultry Diseases, Sichuan Agricultural University.
[0024] 2 media
[0025] Ordinary nutrient broth, nutrient agar medium, configured according to conventional methods [Li Yinglin. Culture medium manual. Jilin: Jilin Science and Technology ...
Embodiment 3
[0039] EXAMPLES Effects of Polygonum saponica Extract on Typical Microorganisms in Broiler Gut and Mortality of Broiler Chickens
[0040] 1 Experimental design
[0041] A single factor experimental design was adopted, with 3 treatments and 4 repetitions for each treatment. Each treatment is as follows:
[0042] Treatment 1: Control group (feeding basal diet without antibiotics)
[0043] Treatment 2: Low-dose Polygonum Polygonum Addition Group (Basic Diet + 0.2wt% Polygonum Polygonum Extract)
[0044] Treatment 3: High-dose Polygonum Polygonum Addition Group (Basic Diet + 0.4wt% Polygonum Polygonum Extract)
[0045] 2 Experimental animals and grouping
[0046] In the experiment, 110 Aviin broilers were selected. They were not divided into groups at the age of 0-21 days, and they were all fed the same brooding feed, in order to establish a relatively stable intestinal microbial flora in the broilers in the same environment. After weighing at the age of 21 days, a total of 9...
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