Ye pseudoalteromonas and application method thereof

A technology of pseudoalteromonas and an application method, applied in the field of pseudoalteromonas yeba, can solve the problems of increased residual bait, outdated breeding technology, polluted breeding water quality, etc., so as to improve the micro-ecological environment, improve the survival rate, The effect of reducing ammonia nitrogen and nitrite

CN106967647AActive Publication Date: 2017-07-21大连市现代农业生产发展服务中心
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
Publication Date
2017-07-21

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Abstract

The invention provides Ye pseudoalteromonas. The classification and name of the Ye pseudoalteromonas is Pseudoalteromonaselyakovii, the name of the bacterial strain is BN-C-11, the bacterial strain is preserved by China General Microbiological Culture Collection Center (CGMCC) on Nov. 11th, 2016, and the preservation number is CGMCCNo. 13265. The invention also provides an application method of the Ye pseudoalteromonas, and is used for producing fungicide for controlling red tide algae. Through biological enzymes generated by fermentation products of the Ye pseudoalteromonas in ponds, reservoirs and sea areas, inundation of the red tide algae can be controlled, and a part of active substances can absorb illumination wave bands necessary for algae growth, so that inundated growth of the red tide algae is controlled, normal growth of aquatic organisms is not influenced, the breeding density is improved, a breeding environment is improved, and the purpose of improving the cultivation yield of the aquatic organisms is reached.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and more specifically relates to a strain of Pseudoalteromonas phylli and its application. Background technique

[0002] Red tide (harmful algal bloom, HAB), also known as red tide, is also called "harmful algae" or "red ghost" internationally. It is a harmful ecological phenomenon in which some phytoplankton, protozoa or bacteria explode and accumulate or cause water discoloration under certain environmental conditions. It mainly includes algal blooms in freshwater systems, general red tides in oceans, brown tides and green tides newly defined in recent years. It is caused by the explosive proliferation of red tide algae in the seaweed family under certain environmental conditions. Seaweeds are a large family, many are very tiny plants and some are single-celled organisms.

[0003] Red tide is a worldwide public hazard, and it occurs frequently in more than 30 countries and regions. First of all...

Examples

Embodiment 1

[0053] Embodiment 1, control the preparation of breeding pond, reservoir or sea area red tide algae bacterial agent

[0054] Step 1: Preparation method of strain liquid medium (pH7.0): 5g peptone, 1g yeast extract powder, 0.01g ferric phosphate, 0.04g NaHCO 3 , 0.02g MgSO 4 ·7H 2 O, 0.02g K 2 HPO 4 , 0.1g NH 4 Cl, dilute to 1000ml with old sea water; in practical application, the pH of the fermentation medium can be 6.5-7.5.

[0055] Step 2: Sterilize the seed liquid culture medium at 120° C. for 20 minutes.

[0056] Step 3: adopt 200L fermenter, and carry out following transformation to fermenter: (1) install red underwater lamp at the bottom of fermenter (the light mainly provides the necessary light source for the growth of Pseudoalteromonas jipvii as energy); (2) An electric heating constant temperature control rod is installed at the bottom of the fermenter; (3) Two external openings are arranged at the bottom of the fermenter, one is used as a ventilation port for ...

Embodiment 2

[0060] Embodiment 2, control the preparation of breeding ponds, reservoirs or sea area red tide algal bacterial agents

[0061] Step 1: Preparation method of strain liquid medium (pH7.0): 5g peptone, 1g yeast extract powder, 0.01g ferric phosphate, 0.04g NaHCO 3 , 0.02g MgSO 4 ·7H 2 O, 0.02g K 2 HPO 4 , 0.1g NH 4 Cl, dilute to 1000ml with old sea water; in practical application, the pH of the fermentation medium can be 6.5-7.5.

[0062] Step 2: Sterilize the seed liquid culture medium at 120° C. for 20 minutes.

[0063] Step 3: Adopt a 200L fermenter, and carry out the following modification on the fermenter: (1) install an electric heating constant temperature control rod at the lower part of the fermenter; (2) set two external openings at the bottom of the fermenter, one as a vent for supplying the fermenter , and the other as an opening for adding culture medium, nutrient salts and strains.

[0064] Add 160L liquid culture medium in the fermenter, then inoculate Pseu...

Embodiment 3

[0067] Embodiment 3, control the preparation of breeding pond, reservoir or sea area red tide algae bacterial agent

[0068] Step 1: Preparation method of strain liquid medium (pH7.0): 5g peptone, 1g yeast extract powder, 0.01g ferric phosphate, 0.04g NaHCO 3 , 0.02g MgSO 4 ·7H 2 O, 0.02g K 2 HPO 4 , 0.1g NH 4 Cl, dilute to 1000ml with old sea water; in practical application, the pH of the fermentation medium can be 6.5-7.5.

[0069] Step 2: Sterilize the seed liquid culture medium at 120° C. for 20 minutes.

[0070] Step 3: adopt 200L fermenter, and carry out following transformation to fermenter: (1) install red underwater lamp at the bottom of fermenter (the light mainly provides the necessary light source for the growth of Pseudoalteromonas jipvii as energy); (2) An electric heating constant temperature control rod is installed at the bottom of the fermenter; (3) Two external openings are arranged at the bottom of the fermenter, one is used as a ventilation port for ...