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A kind of Pseudoalteromonas yipzii and its application method

A pseudoalteromonas and application method technology, applied in the field of Pseudomonas yeba, can solve the problems of polluted breeding water quality, outdated breeding technology, increase of residual bait, etc., to reduce ammonia nitrogen and nitrite, improve micro Ecological environment, the effect of improving the survival rate

Active Publication Date: 2020-06-30
大连市现代农业生产发展服务中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to outdated and imperfect farming techniques, the amount of bait fed is often too large, and the remaining bait increases, which seriously pollutes the water quality of aquaculture.

Method used

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  • A kind of Pseudoalteromonas yipzii and its application method
  • A kind of Pseudoalteromonas yipzii and its application method
  • A kind of Pseudoalteromonas yipzii and its application method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1, control the preparation of breeding pond, reservoir or sea area red tide algae bacterial agent

[0054] Step 1: Preparation method of strain liquid medium (pH7.0): 5g peptone, 1g yeast extract powder, 0.01g ferric phosphate, 0.04g NaHCO 3 , 0.02g MgSO 4 ·7H 2 O, 0.02g K 2 HPO 4 , 0.1g NH 4 Cl, dilute to 1000ml with old sea water; in practical application, the pH of the fermentation medium can be 6.5-7.5.

[0055] Step 2: Sterilize the seed liquid culture medium at 120° C. for 20 minutes.

[0056] Step 3: adopt 200L fermenter, and carry out following transformation to fermenter: (1) install red underwater lamp at the bottom of fermenter (the light mainly provides the necessary light source for the growth of Pseudoalteromonas jipvii as energy); (2) An electric heating constant temperature control rod is installed at the bottom of the fermenter; (3) Two external openings are arranged at the bottom of the fermenter, one is used as a ventilation port for ...

Embodiment 2

[0060] Embodiment 2, control the preparation of breeding ponds, reservoirs or sea area red tide algal bacterial agents

[0061] Step 1: Preparation method of strain liquid medium (pH7.0): 5g peptone, 1g yeast extract powder, 0.01g ferric phosphate, 0.04g NaHCO 3 , 0.02g MgSO 4 ·7H 2 O, 0.02g K 2 HPO 4 , 0.1g NH 4 Cl, dilute to 1000ml with old sea water; in practical application, the pH of the fermentation medium can be 6.5-7.5.

[0062] Step 2: Sterilize the seed liquid culture medium at 120° C. for 20 minutes.

[0063] Step 3: Adopt a 200L fermenter, and carry out the following modification on the fermenter: (1) install an electric heating constant temperature control rod at the lower part of the fermenter; (2) set two external openings at the bottom of the fermenter, one as a vent for supplying the fermenter , and the other serves as an opening for adding culture medium, nutrients and strains.

[0064] Add 160L liquid culture medium in the fermenter, then inoculate Ps...

Embodiment 3

[0067] Embodiment 3, control the preparation of breeding pond, reservoir or sea area red tide algae bacterial agent

[0068] Step 1: Preparation method of strain liquid medium (pH7.0): 5g peptone, 1g yeast extract powder, 0.01g ferric phosphate, 0.04g NaHCO 3 , 0.02g MgSO 4 ·7H 2 O, 0.02g K 2 HPO 4 , 0.1g NH 4 Cl, dilute to 1000ml with old sea water; in practical application, the pH of the fermentation medium can be 6.5-7.5.

[0069] Step 2: Sterilize the seed liquid culture medium at 120° C. for 20 minutes.

[0070] Step 3: adopt 200L fermenter, and carry out following transformation to fermenter: (1) install red underwater lamp at the bottom of fermenter (the light mainly provides the necessary light source for the growth of Pseudoalteromonas jipvii as energy); (2) An electric heating constant temperature control rod is installed at the bottom of the fermenter; (3) Two external openings are arranged at the bottom of the fermenter, one is used as a ventilation port for ...

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Abstract

The invention provides a kind of Pseudoalteromonas elyakovii, which is classified as Pseudoalteromonas elyakovii, and the strain name is BN-C-11. Deposited by the General Microorganism Center of the Preservation Management Committee, the deposit number is CGMCC No.13265. The present invention also provides an application method of the above-mentioned Pseudoalteromonas jipani for the production of bacterial agents for controlling red tide algae. The biological enzymes produced by the fermentation product of Pseudoalteromonas jipii in ponds, reservoirs and sea areas of the present invention can control the flooding of red tide algae, and some active substances can absorb the light band necessary for the growth of algae, so as to achieve the control of red tide The algae blooms and grows without affecting the normal growth of aquatic organisms, thereby increasing the breeding density, improving the breeding environment, and achieving the purpose of increasing the production of aquatic organisms.

Description

technical field [0001] The invention belongs to the field of biotechnology, and more specifically relates to a strain of Pseudoalteromonas phylli and its application. Background technique [0002] Red tide (harmful algal bloom, HAB), also known as red tide, is also called "harmful algae" or "red ghost" internationally. It is a harmful ecological phenomenon in which some phytoplankton, protozoa or bacteria explode and accumulate or cause water discoloration under certain environmental conditions. It mainly includes algal blooms in freshwater systems, general red tides in oceans, brown tides and green tides newly defined in recent years. It is caused by the explosive proliferation of red tide algae in the seaweed family under certain environmental conditions. Seaweeds are a large family, many are very tiny plants and some are single-celled organisms. [0003] Red tide is a worldwide public hazard, and it occurs frequently in more than 30 countries and regions. First of all...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01
CPCC02F3/34C12N1/20C12N1/205C12R2001/01
Inventor 陈文博丁勇孙阳石峰
Owner 大连市现代农业生产发展服务中心
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