Freeze-drying protective additive of pseudoalteromonas bacteria and freeze-drying method
A technology of pseudoalteromonas and freeze-drying protective agent, which is applied in the field of bacterial freeze-drying and storage, can solve the problems of different freeze-drying protective agent and freeze-drying method, and achieves the effect of good protection effect and long storage time.
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Embodiment 1
[0018] In November 2011, "rotting skin syndrome" occurred in japonicus seedlings in a japonicus seedling protection farm in Qingdao City, Shandong Province. A japonicus seedlings with typical symptoms were selected from the breeding pond and brought back to the laboratory of the Yellow Sea Fisheries Research Institute for pathogen isolation. and nurture. Under sterile conditions, pick 3 to 5 diseased sea cucumber seedlings and put them in a sterile petri dish, gently drip wash the lesion several times with sterilized 1.5% NaCl solution, and then scrape it with a sterile scalpel. Take a small amount of tissue from each diseased ginseng lesion in the same new sterile petri dish, cut up and mix these tissues by aseptic operation, and use an inoculation loop to pick a small amount of crushed tissue on tryptone soybean broth agar medium Bacteria were isolated by streaking. Place the streaked medium in a constant temperature incubator at 28°C for 30 hours to obtain a dominant bacte...
Embodiment 2
[0027]In August 2012, "ascites disease" occurred in a semi-smooth tongue sole breeding factory in Weifang City, Shandong Province. The diseased semi-smooth tongue sole fish with obvious symptoms were selected from the breeding pond, packed and oxygenated, and brought back to the laboratory of the Yellow Sea Fisheries Research Institute for pathogen isolation. In the laboratory, aseptically draw the "ascites" of 3 to 5 diseased fish with a disposable syringe into a sterile centrifuge tube, add an equal volume of sterile NaCl solution with a concentration of 1.5%, and oscillate evenly . Use a pipette to draw 100 μL of the mixture and add it to a tryptone soybean broth agar medium, and spread the culture. After the coated medium was placed in a constant temperature incubator at 28° C. for 36 hours, three dominant bacterial colonies with different forms were obtained. Single colonies of these three bacteria were picked and transferred to a new tryptone soybean broth agar medium f...
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Application Information
- IPC
- C12N1/04; C12R1/01
- Inventors
- 王印庚; 张正