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Pseudoalteromonas fivipulchra with algae dissolving capacity and application of pseudoalteromonas to karenia mikimotoi red tide

A technology of pseudoalteromonas and alteromonas, which is applied in the field of environmental microorganisms, can solve the problems of negative impact on benthic organisms, high mechanical energy consumption, and ineffective killing of K. mikielii, etc., and achieves algal dissolution. Good effect, good application prospect, environment-friendly effect

Active Publication Date: 2019-09-17
FUZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Physical methods consume high mechanical energy and are likely to have negative impacts on benthic organisms, and cannot fundamentally control red tides
However, there is no report on the algae-dissolving bacteria and bacterial agents that can effectively kill Karenia militaris

Method used

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  • Pseudoalteromonas fivipulchra with algae dissolving capacity and application of pseudoalteromonas to karenia mikimotoi red tide
  • Pseudoalteromonas fivipulchra with algae dissolving capacity and application of pseudoalteromonas to karenia mikimotoi red tide
  • Pseudoalteromonas fivipulchra with algae dissolving capacity and application of pseudoalteromonas to karenia mikimotoi red tide

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Experimental program
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Effect test

Embodiment 1

[0035] The screening of embodiment 1 algae-lytic bacteria

[0036] In the late stage of a red tide event of Karenia militaris in Lianjiang County, Fujian Province, the surface seawater samples were serially diluted 10 times, and 0.1 mL of each dilution was spread on 2216E solid medium, and then incubated at 25 °C. Colonies of different shapes, colors and sizes were taken for isolation and purification of single colonies. The obtained colony cultures were inoculated into 2216E liquid medium for cultivation at a temperature of 25 °C and a rotational speed of 150 r / min. Then the bacterial liquid was co-cultured with the algae liquid of Karenia militaris at a ratio of 2% v / v. After 48 hours, the strain that made the algae liquid appear yellow was considered as alginolytic bacteria. A bacterium strain with the best algicidal effect on Karenia mittelis was selected for further research, and the strain was code-named FDHY-MQ5.

[0037] Karenia militaris culture conditions: Karenia ...

Embodiment 2

[0039] Example 2 Pseudoalteromonas FDHY-MQ5 strain identification

[0040] The morphological observation and physiological and biochemical reactions of the FDHY-MQ5 strain showed that the FDHY-MQ5 bacteria had the following biological characteristics: rod-shaped, single, Gram staining was negative, the colony was round or oval, opaque, and the colony was Pale yellow, smooth and moist surface, viscous when provoked ( figure 1 ).

[0041]The 16s rRNA of the FDHY-MQ5 strain was amplified, and the gene sequence was cloned and sequenced, and the results showed that it was related to Pseudoalteromonas flavipulchra strain CSMA-N1 is 99% homologous, so it was identified as Pseudoalteromonas Pseudoalteromonas sp., named Alteromonas FDHY-MQ5. The strain was deposited in the China Center for Type Culture Collection on May 20, 2019, with the collection number: CCTCC NO: M 2019371. The accession number of the strain's 16s rRNA sequence in GenBank is MK968367.

Embodiment 3

[0042] Example 3 The algicidal effect of different concentrations of Alteromonas FDHY-MQ5 on Karenia militaris

[0043] The products of the primary culture of alginolytic bacteria FDHY-MQ5 were co-cultured with the algae liquid at volume concentrations of 0.5%, 1%, and 2%. A blank control was set, and the control group was the algae liquid of the same period added with 0.5%, 1%, and 2% volume sterile 2216E medium. Both the experimental group and the control group were set up with 3 repetitions. Samples were taken at 0 h, 6 h, 24 h, and 48 h, and 2 mL of culture solution was taken each time to count the algae cell concentration and further calculate the algae dissolving rate (Table 1). figure 2 , see the effect of different concentrations of algae image 3 .

[0044] Table 1 Algae-dissolving efficiency of different concentrations of alg-dissolving bacteria on Karenia mirkei

[0045]

[0046] It can be seen from Table 1 that the product of the fermentation culture of the...

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Abstract

The invention provides pseudoalteromonas fivipulchra with algae dissolving capacity and application of the pseudoalteromonas to karenia mikimotoi red tide and belongs to the microbiological field of harmful red tide treatment. The algae-dissolving bacterium is named as (pseudoalteromonas fivipulchra) FDHY-MQ5 and is preserved at the China Center for Type Culture Collection on May 20, 2019 with the preservation number CCTCC NO: M 2019371. The fermentation liquor of the strain is added into karenia mikimotoi in an exponential growth phase according to the volume ratio of 1%, and the algae killing rate is 94.3-98.2% in 24 hours. After optimization of fermentation conditions, 18-20 g of bacterium powder can be produced per liter of bacterium solution, and under the condition of 24-hour treatment, the karenia mikimotoi dissolution rate is 99.67-100% by adding 0.04% of bacterial powder by mass.

Description

technical field [0001] The invention relates to the field of environmental microorganisms, in particular to a strain of pseudoalteromonas with algae-dissolving ability and its application. Background technique [0002] In recent decades, the frequency and scale of red tides caused by eutrophication in my country's coastal waters have shown a significant increase. The latest investigation and research results show that the increase in the concentration of carbon dioxide and water temperature in seawater caused by global climate change and further deterioration of the coastal environment in recent years has had a great impact on the physiology of the main harmful red tide algae, resulting in the main cause of red tide species being non-toxic and harmful. The species gradually evolved into a variety of toxic and harmful dinoflagellate red tide species. At present, Karenia michaelii has evolved into the largest toxic and harmful red tide algae species with the largest outbreak ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F103/08
CPCC02F3/34C02F2103/08C12N1/20C12N1/205C12R2001/01
Inventor 石新国陈剑锋谢友坪刘乐冕郑向南马瑞娟
Owner FUZHOU UNIV
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