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Ocean microorganism low-temperature amylase and producing strain pseudoalteromonas GS230 thereof

A technology of Pseudomonas alternata, low-temperature amylase, applied in the direction of microorganisms, microorganism-based methods, biochemical equipment and methods, etc.

Inactive Publication Date: 2008-06-18
HUAIHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report of low-temperature amylase produced by Pseudomonas alternata marina in China

Method used

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  • Ocean microorganism low-temperature amylase and producing strain pseudoalteromonas GS230 thereof
  • Ocean microorganism low-temperature amylase and producing strain pseudoalteromonas GS230 thereof
  • Ocean microorganism low-temperature amylase and producing strain pseudoalteromonas GS230 thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0097] Example 1. Refer to Figure 1-17. A kind of Pseudomonas alternata GS230 (Pseudoalteromonassp.GS230) CGMCC NO.2156. The strain has the following characteristics: it is a Gram-negative bacillus with capsule and no spores, and its size is 2.2-2.7μm×0.7-1.3μm; the colony characteristics on starch solid medium: 4mm-7mm in diameter, white and moist , smooth edges, protruding middle, easy to pick, and can produce obvious transparent circles; its physiological and biochemical characteristics are that the oxidase, catalase, and VP tests of the bacteria are all positive, and cannot ferment glucose, lysine decarboxylase, MR test is negative, can produce indole but not H 2 S; The strain can utilize lactose, fructose, galactose, maltose, citrate, pyruvate, alanine, glutamic acid, but cannot utilize sucrose, cellobiose, melibiose.

Embodiment 2

[0098] Example 2. The Pseudomonas alternata GS230 CGMCC NO.2156 described in Example 1 has a growth temperature range of 0°C-30°C and belongs to psychrotrophic bacteria; the growth pH range is 5-10; the growth NaCl concentration is in the range of 1%- 9%.

Embodiment 3

[0099] Example 3. For Pseudomonas alternans GS230 CGMCC NO.2156 described in Example 1, the suitable growth temperature of this bacterium is 25° C., the suitable growth pH is 7.0, and the suitable growth NaCl concentration is 5%.

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Abstract

The present invention provides a Pseudoalteromonassp GS230 CGMCC NO.2156. The strain can grow under 4 DEG C; the growth temperature range of the strain is between 0 DEG C and 30 DEG C; the best growth temperature is 25 DEG C; the growth pH range is between 5 and 10; the best growth pH is 7.0; the growth NaCl concentration is between 1 percent and 9 percent; the best growth NaCl concentration is 5 percent; the strain belongs to a marine coolness tolerant strain. The present invention also discloses a method of using the Pseudoalteromonassp to produce low-temperature amylase and a low-temperature amylase product obtained by the method. The low-temperature amylase can be applied to the baking industry and used for producing flavor substances and can be mixed with protease to be washed directly under room temperature, which saves energy source; the low-temperature amylase also has wide application prospect at the fields of feed, weaving, environmental protection, medicine, etc.

Description

technical field [0001] The present invention relates to a kind of Pseudomonas alternanas GS230 (Pseudoalteromonas sp.GS230) CGMCC NO.2156 (preserved in China Microorganism Culture Preservation Management Committee on September 26, 2007) isolated from the seawater of Lianyungang sea area, Yellow Sea, China General Microorganism Center CGMCC, the preservation number is CGMCC NO.2156); the present invention also relates to the method and product of the low-temperature amylase produced by Pseudomonas alternata. Background technique [0002] Amylase is a general term for a class of enzymes that can catalyze the hydrolysis of starch into glucose, maltose and other oligosaccharides. It is widely found in animals, plants and microorganisms. Widely used in papermaking, food, pharmaceutical industry and detergent industry. Ordinary amylase and high-temperature amylase have high temperature in the starch hydrolysis process, and the raw materials are easy to deteriorate, which restrict...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/26C12R1/38
Inventor 吕明生王淑军刘红飞房耀维刘殊陈丽
Owner HUAIHAI INST OF TECH
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